Trypanosoma cruzi in the chicken model : Chagas-like heart disease in the absence of parasitism
Gorde:
| Egile nagusia: | |
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| Argitaratze data: | 2011 |
| Beste egile batzuk: | , , , , , , , , , , |
| Formatua: | Article |
| Hizkuntza: | eng |
| Baliabidea: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/33957 |
Gaia: | Background: The administration of anti-trypanosome nitroderivatives curtails Trypanosoma cruzi infection in Chagas disease patients, but does not prevent destructive lesions in the heart. This observation suggests that an effective treatment for the disease requires understanding its pathogenesis. Methodology/Principal Findings: To understand the origin of clinical manifestations of the heart disease we used a chicken model system in which infection can be initiated in the egg, but parasite persistence is precluded. T. cruzi inoculation into the air chamber of embryonated chicken eggs generated chicks that retained only the parasite mitochondrial kinetoplast DNA minicircle in their genome after eight days of gestation. Crossbreeding showed that minicircles were transferred vertically via the germ line to chicken progeny. Minicircle integration in coding regions was shown by targeted-primer thermal asymmetric interlaced PCR, and detected by direct genomic analysis. The kDNA-mutated chickens died with arrhythmias, shortness of breath, cyanosis and heart failure. These chickens with cardiomyopathy had rupture of the dystrophin and other genes that regulate cell growth and differentiation. Tissue pathology revealed inflammatory dilated cardiomegaly whereby immune system mononuclear cells lyse parasite-free target heart fibers. The heart cell destruction implicated a thymus-dependent, autoimmune; self-tissue rejection carried out by CD45+, CD8cd+, and CD8a lymphocytes. Conclusions/Significance: These results suggest that genetic alterations resulting from kDNA integration in the host genome lead to autoimmune-mediated destruction of heart tissue in the absence of T. cruzi parasites. |
Antzeko izenburuak: Trypanosoma cruzi in the chicken model : Chagas-like heart disease in the absence of parasitism
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- Emerging Chagas Disease : trophic network and cycle of transmission of Trypanosoma cruzi from palm trees in the Amazon
- The immunology of experimental Chagas' disease III. rejection of allogeneic heart cells in vitro
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- The repetitive cytoskeletal protein H49 of Trypanosoma cruzi is a calpain-like protein located at the flagellum attachment zone
- Subproteômica de Trypanosoma cruzi : proteínas básicas e fosfoproteoma
