Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model
Tallennettuna:
| Päätekijä: | |
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| Julkaisupäivä: | 2024 |
| Muut tekijät: | , , , , , , , , , , , , , , |
| Aineistotyyppi: | Article |
| Kieli: | eng |
| Lähde: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/51697 https://doi.org/10.3390/pharmaceutics16091177 https://orcid.org/0000-0003-3067-4842 https://orcid.org/0000-0002-7136-7059 https://orcid.org/0000-0002-0819-0756 https://orcid.org/0000-0003-3323-5300 https://orcid.org/0000-0001-8013-8250 https://orcid.org/0000-0002-4289-8678 https://orcid.org/0000-0002-2098-4401 https://orcid.org/0000-0002-9814-5827 https://orcid.org/0000-0002-9873-3098 https://orcid.org/0000-0002-7223-037X https://orcid.org/0000-0002-5154-7263 https://orcid.org/0000-0002-6974-0889 https://orcid.org/0000-0002-2137-9588 https://orcid.org/0000-0003-4711-7583 |
Yhteenveto: | Melanoma, the most aggressive form of skin cancer, presents a major clinical challenge due to its tendency to metastasize and recalcitrance to traditional therapies. Despite advances in surgery, chemotherapy, and radiotherapy, the outlook for advanced melanoma remains bleak, reinforcing the urgent need for more effective treatments. Photodynamic therapy (PDT) has emerged as a promising alternative, leading to targeted tumor destruction with minimal harm to surrounding tissues. In this study, the direct and abscopal antitumor effects of PDT in a bilateral murine melanoma model were evaluated. Although only one of the two tumors was treated, effects were observed in both. Our findings revealed significant changes in systemic inflammation and alterations in CD4+ and CD8+ T cell populations in treated groups, as evidenced by blood analyses and flow cytometry. High-throughput RNA sequencing (RNA-Seq) further unveiled shifts in gene expression profiles in both treated and untreated tumors. This research sheds light on the novel antitumor and abscopal effects of nanoemulsion of aluminum chloride phthalocyanine (AlPcNE)-mediated PDT in melanoma, highlighting the potential of different PDT protocols to modulate immune responses and to achieve more effective and targeted cancer treatments |
| _version_ | 1871442421397585920 |
|---|---|
| author | Morais, José Athayde Vasconcelos |
| author2 | Barros, Pedro Henrique Aragão Brígido, Marcelo de Macedo Marina, Clara Luna Bocca, Anamélia Lorenzetti Mariano, André de Lima e Silva Souza, Paulo Eduardo Narcizo de Paiva, Karen Letycia Rodrigues de Simões, Marina Mesquita Báo, Sonia Nair Camargo, Luana Cristina Longo, João Paulo Figueiró Morais, Amanda Alencar Cabral Azevedo, Ricardo Bentes de Fonseca, Marcio José Poças Muehlmann, Luis Alexandre |
| author2_role | author author author author author author author author author author author author author author author |
| author_browse | Azevedo, Ricardo Bentes de Barros, Pedro Henrique Aragão Bocca, Anamélia Lorenzetti Brígido, Marcelo de Macedo Báo, Sonia Nair Camargo, Luana Cristina Fonseca, Marcio José Poças Longo, João Paulo Figueiró Mariano, André de Lima e Silva Marina, Clara Luna Morais, Amanda Alencar Cabral Morais, José Athayde Vasconcelos Muehlmann, Luis Alexandre Paiva, Karen Letycia Rodrigues de Simões, Marina Mesquita Souza, Paulo Eduardo Narcizo de |
| author_facet | Morais, José Athayde Vasconcelos Barros, Pedro Henrique Aragão Brígido, Marcelo de Macedo Marina, Clara Luna Bocca, Anamélia Lorenzetti Mariano, André de Lima e Silva Souza, Paulo Eduardo Narcizo de Paiva, Karen Letycia Rodrigues de Simões, Marina Mesquita Báo, Sonia Nair Camargo, Luana Cristina Longo, João Paulo Figueiró Morais, Amanda Alencar Cabral Azevedo, Ricardo Bentes de Fonseca, Marcio José Poças Muehlmann, Luis Alexandre |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.unb.br/bitstream/10482/51697/1/ARTIGO_DirectAbscopalAntitumor.pdf http://repositorio.unb.br/bitstream/10482/51697/2/license.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.affiliation.pt_BR.fl_str_mv | University of Brasilia, Faculty of Ceilandia, Laboratory of Nanoscience and Immunology University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Gene Regulation and Mutagenesis University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Gene Regulation and Mutagenesis University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Gene Regulation and Mutagenesis University of Brasília, Institute of Biological Sciences, Laboratory of Applied Immunology University of Brasília, Institute of Biological Sciences, Laboratory of Applied Immunology University of Brasilia, Institute of Physics, Laboratory for Softwares and Physics Instrumentation Development University of Brasilia, Institute of Physics, Laboratory for Softwares and Physics Instrumentation Development University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and Microanalysis University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and Microanalysis University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and Microanalysis University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Gene Regulation and Mutagenesis |
| dc.contributor.author.fl_str_mv | Morais, José Athayde Vasconcelos Barros, Pedro Henrique Aragão Brígido, Marcelo de Macedo Marina, Clara Luna Bocca, Anamélia Lorenzetti Mariano, André de Lima e Silva Souza, Paulo Eduardo Narcizo de Paiva, Karen Letycia Rodrigues de Simões, Marina Mesquita Báo, Sonia Nair Camargo, Luana Cristina Longo, João Paulo Figueiró Morais, Amanda Alencar Cabral Azevedo, Ricardo Bentes de Fonseca, Marcio José Poças Muehlmann, Luis Alexandre |
| dc.date.accessioned.fl_str_mv | 2025-02-20T14:34:30Z |
| dc.date.available.fl_str_mv | 2025-02-20T14:34:30Z |
| dc.date.issued.fl_str_mv | 2024-09-06 |
| dc.identifier.citation.fl_str_mv | MORAIS, José Athayde Vasconcelos et al. Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model. Pharmaceutics, [S. l.], v. 16, n. 9, 1177, 2024. DOI: https://doi.org/10.3390/pharmaceutics16091177. Disponível em: https://www.mdpi.com/1999-4923/16/9/1177. Acesso em: 17 fev. 2025. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/pharmaceutics16091177 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0003-3067-4842 https://orcid.org/0000-0002-7136-7059 https://orcid.org/0000-0002-0819-0756 https://orcid.org/0000-0003-3323-5300 https://orcid.org/0000-0001-8013-8250 https://orcid.org/0000-0002-4289-8678 https://orcid.org/0000-0002-2098-4401 https://orcid.org/0000-0002-9814-5827 https://orcid.org/0000-0002-9873-3098 https://orcid.org/0000-0002-7223-037X https://orcid.org/0000-0002-5154-7263 https://orcid.org/0000-0002-6974-0889 https://orcid.org/0000-0002-2137-9588 https://orcid.org/0000-0003-4711-7583 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/51697 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Sistema imunológico Imunoterapia Nanoemulsão Melanoma |
| dc.title.pt_BR.fl_str_mv | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Melanoma, the most aggressive form of skin cancer, presents a major clinical challenge due to its tendency to metastasize and recalcitrance to traditional therapies. Despite advances in surgery, chemotherapy, and radiotherapy, the outlook for advanced melanoma remains bleak, reinforcing the urgent need for more effective treatments. Photodynamic therapy (PDT) has emerged as a promising alternative, leading to targeted tumor destruction with minimal harm to surrounding tissues. In this study, the direct and abscopal antitumor effects of PDT in a bilateral murine melanoma model were evaluated. Although only one of the two tumors was treated, effects were observed in both. Our findings revealed significant changes in systemic inflammation and alterations in CD4+ and CD8+ T cell populations in treated groups, as evidenced by blood analyses and flow cytometry. High-throughput RNA sequencing (RNA-Seq) further unveiled shifts in gene expression profiles in both treated and untreated tumors. This research sheds light on the novel antitumor and abscopal effects of nanoemulsion of aluminum chloride phthalocyanine (AlPcNE)-mediated PDT in melanoma, highlighting the potential of different PDT protocols to modulate immune responses and to achieve more effective and targeted cancer treatments |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_9ccc9df5205cc1a876af22b3dffc8cfd |
| identifier_str_mv | MORAIS, José Athayde Vasconcelos et al. Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model. Pharmaceutics, [S. l.], v. 16, n. 9, 1177, 2024. DOI: https://doi.org/10.3390/pharmaceutics16091177. Disponível em: https://www.mdpi.com/1999-4923/16/9/1177. Acesso em: 17 fev. 2025. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/51697 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Morais, José Athayde VasconcelosBarros, Pedro Henrique AragãoBrígido, Marcelo de MacedoMarina, Clara LunaBocca, Anamélia LorenzettiMariano, André de Lima e SilvaSouza, Paulo Eduardo Narcizo dePaiva, Karen Letycia Rodrigues deSimões, Marina MesquitaBáo, Sonia NairCamargo, Luana CristinaLongo, João Paulo FigueiróMorais, Amanda Alencar CabralAzevedo, Ricardo Bentes deFonseca, Marcio José PoçasMuehlmann, Luis AlexandreUniversity of Brasilia, Faculty of Ceilandia, Laboratory of Nanoscience and ImmunologyUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Gene Regulation and MutagenesisUniversity of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Gene Regulation and MutagenesisUniversity of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Gene Regulation and MutagenesisUniversity of Brasília, Institute of Biological Sciences, Laboratory of Applied ImmunologyUniversity of Brasília, Institute of Biological Sciences, Laboratory of Applied ImmunologyUniversity of Brasilia, Institute of Physics, Laboratory for Softwares and Physics Instrumentation DevelopmentUniversity of Brasilia, Institute of Physics, Laboratory for Softwares and Physics Instrumentation DevelopmentUniversity of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and MicroanalysisUniversity of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and MicroanalysisUniversity of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and MicroanalysisUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and NanobiotechnologyUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and NanobiotechnologyUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and NanobiotechnologyUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Nanoscience and NanobiotechnologyUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics and Morphology, Laboratory of Gene Regulation and Mutagenesis2025-02-20T14:34:30Z2025-02-20T14:34:30Z2024-09-06MORAIS, José Athayde Vasconcelos et al. Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model. Pharmaceutics, [S. l.], v. 16, n. 9, 1177, 2024. DOI: https://doi.org/10.3390/pharmaceutics16091177. Disponível em: https://www.mdpi.com/1999-4923/16/9/1177. Acesso em: 17 fev. 2025.http://repositorio.unb.br/handle/10482/51697https://doi.org/10.3390/pharmaceutics16091177https://orcid.org/0000-0003-3067-4842https://orcid.org/0000-0002-7136-7059https://orcid.org/0000-0002-0819-0756https://orcid.org/0000-0003-3323-5300https://orcid.org/0000-0001-8013-8250https://orcid.org/0000-0002-4289-8678https://orcid.org/0000-0002-2098-4401https://orcid.org/0000-0002-9814-5827https://orcid.org/0000-0002-9873-3098https://orcid.org/0000-0002-7223-037Xhttps://orcid.org/0000-0002-5154-7263https://orcid.org/0000-0002-6974-0889https://orcid.org/0000-0002-2137-9588https://orcid.org/0000-0003-4711-7583engMDPIThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).info:eu-repo/semantics/openAccessDirect and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSistema imunológicoImunoterapiaNanoemulsãoMelanomaMelanoma, the most aggressive form of skin cancer, presents a major clinical challenge due to its tendency to metastasize and recalcitrance to traditional therapies. Despite advances in surgery, chemotherapy, and radiotherapy, the outlook for advanced melanoma remains bleak, reinforcing the urgent need for more effective treatments. Photodynamic therapy (PDT) has emerged as a promising alternative, leading to targeted tumor destruction with minimal harm to surrounding tissues. In this study, the direct and abscopal antitumor effects of PDT in a bilateral murine melanoma model were evaluated. Although only one of the two tumors was treated, effects were observed in both. Our findings revealed significant changes in systemic inflammation and alterations in CD4+ and CD8+ T cell populations in treated groups, as evidenced by blood analyses and flow cytometry. High-throughput RNA sequencing (RNA-Seq) further unveiled shifts in gene expression profiles in both treated and untreated tumors. This research sheds light on the novel antitumor and abscopal effects of nanoemulsion of aluminum chloride phthalocyanine (AlPcNE)-mediated PDT in melanoma, highlighting the potential of different PDT protocols to modulate immune responses and to achieve more effective and targeted cancer treatmentsFaculdade de Ciências e Tecnologias em Saúde (FCTS) – Campus UnB CeilândiaInstituto de Ciências Biológicas (IB)Departamento de Genética e Morfologia (IB GEM)Departamento de Biologia Celular (IB CEL)Instituto de Física (IF)Programa de Pós-Graduação em Biologia Animalreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_DirectAbscopalAntitumor.pdfARTIGO_DirectAbscopalAntitumor.pdfapplication/pdf8237080http://repositorio.unb.br/bitstream/10482/51697/1/ARTIGO_DirectAbscopalAntitumor.pdf114e6bff628d17c96876b1adc9d2ba24MD51open accessLICENSElicense.txtlicense.txttext/plain102http://repositorio.unb.br/bitstream/10482/51697/2/license.txtaed4704d04bb260d4decd80db311aaa5MD52open access10482/516972025-02-20 11:34:32.559open accessoai:repositorio.unb.br:10482/51697U3VibWlzc8OjbyBlZmV0aXZhZGEgZGUgYWNvcmRvIGNvbSBsaWNlbsOnYSBjb25jZWRpZGEgcGVsbyBhdXRvciBlL291IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcy4KRepositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2025-02-20T14:34:32Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model Morais, José Athayde Vasconcelos Sistema imunológico Imunoterapia Nanoemulsão Melanoma |
| status_str | publishedVersion |
| title | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| title_full | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| title_fullStr | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| title_full_unstemmed | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| title_short | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| title_sort | Direct and abscopal antitumor responses elicited by AlPcNE-mediated photodynamic therapy in a Murine melanoma model |
| topic | Sistema imunológico Imunoterapia Nanoemulsão Melanoma |
| url | http://repositorio.unb.br/handle/10482/51697 https://doi.org/10.3390/pharmaceutics16091177 https://orcid.org/0000-0003-3067-4842 https://orcid.org/0000-0002-7136-7059 https://orcid.org/0000-0002-0819-0756 https://orcid.org/0000-0003-3323-5300 https://orcid.org/0000-0001-8013-8250 https://orcid.org/0000-0002-4289-8678 https://orcid.org/0000-0002-2098-4401 https://orcid.org/0000-0002-9814-5827 https://orcid.org/0000-0002-9873-3098 https://orcid.org/0000-0002-7223-037X https://orcid.org/0000-0002-5154-7263 https://orcid.org/0000-0002-6974-0889 https://orcid.org/0000-0002-2137-9588 https://orcid.org/0000-0003-4711-7583 |