Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil
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| Publication Date: | 2004 |
| Other Authors: | , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/26148 https://dx.doi.org/10.1590/S0074-02762004000800016 |
Summary: | In the context of universal access to antiretroviral therapy, the surveillance of human immunodeficiency virus type 1 (HIV-1) genetic diversity and resistance becomes pivotal. In this work our purpose was to describe the genetic variability; prevalence of drug-resistance mutations; and genotypic resistance profiles in HIV-1 infected individuals under antiretroviral treatment, from the Federal District, Brasília, Central Brazil. The entire viral protease and codons 19 to 234 of the reverse transcriptase gene from 45 HIV-1 isolates were amplified and sequenced for subtyping and genotyping. By phylogenetic analysis, 96% of the samples clustered with subtype B and the remaining 4% with HIV-1 subtype F sequences. One major protease inhibitor resistance-associated mutation, I50V, was detected in 38% of the samples. Minor mutations were also found at the protease gene: L10I/V (7%), K20M (2%), M36I (11%), L63P (20%), A71T (2%), and V77I (7%). Many mutations associated with reduced susceptibility to nucleoside or non-nucleoside reverse transcriptase inhibitors were detected: M41L (11%), E44D (4%), D67N (11%), T69D (2%), K70R (11%), L74V (2%), L100I (4%), K103N (18%), V118I (9%), Y181C (11%), M184V (18%), G190A (4%), T215Y (4%), and K219E (4%). This study has shown that 84% of the studied population from the Federal District, showing evidences of therapy failure, presented viral genomic mutations associated with drug resistance. The main antiretrovirals to which this population showed resistance were the PI amprenavir (38%), the NNRTIs delavirdine, nevirapine (31%), and efavirenz (24%), and the NRTIs lamivudine (18%), abacavir, and zidovudine (13%). |
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|---|---|
| author | Cerqueira, Daniela M |
| author2 | Amorim, Regina MS Silva, Ruiter R Camara, Geni NL Brígido, Marcelo de Macedo Martins, Cláudia RF |
| author2_role | author author author author author |
| author_browse | Amorim, Regina MS Brígido, Marcelo de Macedo Camara, Geni NL Cerqueira, Daniela M Martins, Cláudia RF Silva, Ruiter R |
| author_facet | Cerqueira, Daniela M Amorim, Regina MS Silva, Ruiter R Camara, Geni NL Brígido, Marcelo de Macedo Martins, Cláudia RF |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.unb.br/bitstream/10482/26148/1/v99n8a16.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Cerqueira, Daniela M Amorim, Regina MS Silva, Ruiter R Camara, Geni NL Brígido, Marcelo de Macedo Martins, Cláudia RF |
| dc.date.accessioned.fl_str_mv | 2017-12-07T04:39:12Z |
| dc.date.available.fl_str_mv | 2017-12-07T04:39:12Z |
| dc.date.issued.fl_str_mv | 2004 |
| dc.identifier.citation.fl_str_mv | Mem. Inst. Oswaldo Cruz,v.99,n.8,p.877-882,2004 |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1590/S0074-02762004000800016 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/26148 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | HIV (Vírus) Genotipagem Resistência antirretroviral protease transcriptase reversa Brasil |
| dc.title.pt_BR.fl_str_mv | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | In the context of universal access to antiretroviral therapy, the surveillance of human immunodeficiency virus type 1 (HIV-1) genetic diversity and resistance becomes pivotal. In this work our purpose was to describe the genetic variability; prevalence of drug-resistance mutations; and genotypic resistance profiles in HIV-1 infected individuals under antiretroviral treatment, from the Federal District, Brasília, Central Brazil. The entire viral protease and codons 19 to 234 of the reverse transcriptase gene from 45 HIV-1 isolates were amplified and sequenced for subtyping and genotyping. By phylogenetic analysis, 96% of the samples clustered with subtype B and the remaining 4% with HIV-1 subtype F sequences. One major protease inhibitor resistance-associated mutation, I50V, was detected in 38% of the samples. Minor mutations were also found at the protease gene: L10I/V (7%), K20M (2%), M36I (11%), L63P (20%), A71T (2%), and V77I (7%). Many mutations associated with reduced susceptibility to nucleoside or non-nucleoside reverse transcriptase inhibitors were detected: M41L (11%), E44D (4%), D67N (11%), T69D (2%), K70R (11%), L74V (2%), L100I (4%), K103N (18%), V118I (9%), Y181C (11%), M184V (18%), G190A (4%), T215Y (4%), and K219E (4%). This study has shown that 84% of the studied population from the Federal District, showing evidences of therapy failure, presented viral genomic mutations associated with drug resistance. The main antiretrovirals to which this population showed resistance were the PI amprenavir (38%), the NNRTIs delavirdine, nevirapine (31%), and efavirenz (24%), and the NRTIs lamivudine (18%), abacavir, and zidovudine (13%). |
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| id | UNB_7e4dba6dfccc613b4e18e2e7b9aecc78 |
| identifier_str_mv | Mem. Inst. Oswaldo Cruz,v.99,n.8,p.877-882,2004 |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
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| oai_identifier_str | oai:repositorio.unb.br:10482/26148 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher.none.fl_str_mv | Instituto Oswaldo Cruz, Ministério da Saúde |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
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| spelling | Cerqueira, Daniela MAmorim, Regina MSSilva, Ruiter RCamara, Geni NLBrígido, Marcelo de MacedoMartins, Cláudia RF2017-12-07T04:39:12Z2017-12-07T04:39:12Z2004Mem. Inst. Oswaldo Cruz,v.99,n.8,p.877-882,2004http://repositorio.unb.br/handle/10482/26148https://dx.doi.org/10.1590/S0074-02762004000800016In the context of universal access to antiretroviral therapy, the surveillance of human immunodeficiency virus type 1 (HIV-1) genetic diversity and resistance becomes pivotal. In this work our purpose was to describe the genetic variability; prevalence of drug-resistance mutations; and genotypic resistance profiles in HIV-1 infected individuals under antiretroviral treatment, from the Federal District, Brasília, Central Brazil. The entire viral protease and codons 19 to 234 of the reverse transcriptase gene from 45 HIV-1 isolates were amplified and sequenced for subtyping and genotyping. By phylogenetic analysis, 96% of the samples clustered with subtype B and the remaining 4% with HIV-1 subtype F sequences. One major protease inhibitor resistance-associated mutation, I50V, was detected in 38% of the samples. Minor mutations were also found at the protease gene: L10I/V (7%), K20M (2%), M36I (11%), L63P (20%), A71T (2%), and V77I (7%). Many mutations associated with reduced susceptibility to nucleoside or non-nucleoside reverse transcriptase inhibitors were detected: M41L (11%), E44D (4%), D67N (11%), T69D (2%), K70R (11%), L74V (2%), L100I (4%), K103N (18%), V118I (9%), Y181C (11%), M184V (18%), G190A (4%), T215Y (4%), and K219E (4%). This study has shown that 84% of the studied population from the Federal District, showing evidences of therapy failure, presented viral genomic mutations associated with drug resistance. The main antiretrovirals to which this population showed resistance were the PI amprenavir (38%), the NNRTIs delavirdine, nevirapine (31%), and efavirenz (24%), and the NRTIs lamivudine (18%), abacavir, and zidovudine (13%).Em processamentoInstituto Oswaldo Cruz, Ministério da SaúdeAntiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHIV (Vírus)GenotipagemResistência antirretroviralproteasetranscriptase reversaBrasilinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALv99n8a16.pdfapplication/pdf122593http://repositorio.unb.br/bitstream/10482/26148/1/v99n8a16.pdf218e20810b81f1a93a46386b572c43c9MD51open access10482/261482024-08-28 16:04:07.368open accessoai:repositorio.unb.br:10482/26148Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:07Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil Cerqueira, Daniela M HIV (Vírus) Genotipagem Resistência antirretroviral protease transcriptase reversa Brasil |
| status_str | publishedVersion |
| title | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| title_full | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| title_fullStr | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| title_full_unstemmed | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| title_short | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| title_sort | Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil |
| topic | HIV (Vírus) Genotipagem Resistência antirretroviral protease transcriptase reversa Brasil |
| url | http://repositorio.unb.br/handle/10482/26148 https://dx.doi.org/10.1590/S0074-02762004000800016 |