Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations
Kaydedildi:
| Yazar: | |
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| Yayın Tarihi: | 2013 |
| Diğer Yazarlar: | , |
| Materyal Türü: | Article |
| Dil: | eng |
| Kaynak: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/23744 https://dx.doi.org/10.1186/1471-2180-13-22 |
Özet: | Background: Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown inconsistent results. In this work, two different collections of DAEC possessing Afa/Dr genes, from children and adults, were studied regarding characteristics potentially associated to virulence. Results: DAEC strains were recovered in similar frequencies from diarrheic and asymptomatic children, and more frequently from adults with diarrhea (P < 0.01) than from asymptomatic adults. Association with diarrhea (P < 0.05) was found for SAT-positive strains recovered from children and for curli-positive strains recovered from adults. Mixed biofilms involving DAEC and a Citrobacter freundii strain have shown an improved ability to form biofilms in relation to the monocultures. Control strains have shown a greater diversity of Afa/Dr adhesins and higher frequencies of cellulose, TTSS, biofilm formation and induction of IL-8 secretion than strains from cases of diarrhea in children. Conclusions: DAEC strains possessing Afa/Dr genes isolated from children and adults represent two different bacterial populations. DAEC strains carrying genes associated to virulence can be found as part of the normal microbiota present in asymptomatic children. |
| _version_ | 1871442291044909056 |
|---|---|
| author | Almeida, Rosane Mansan |
| author2 | Pereira, Alex Leite Giugliano, Loreny Gimenes |
| author2_role | author author |
| author_browse | Almeida, Rosane Mansan Giugliano, Loreny Gimenes Pereira, Alex Leite |
| author_facet | Almeida, Rosane Mansan Pereira, Alex Leite Giugliano, Loreny Gimenes |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/23744/1/ARTIGO_DiffuselyAdherentEscherichiacoli.pdf http://repositorio2.unb.br/jspui/bitstream/10482/23744/2/license.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Almeida, Rosane Mansan Pereira, Alex Leite Giugliano, Loreny Gimenes |
| dc.date.accessioned.fl_str_mv | 2017-06-24T13:05:03Z |
| dc.date.available.fl_str_mv | 2017-06-24T13:05:03Z |
| dc.date.issued.fl_str_mv | 2013-02-01 |
| dc.identifier.citation.fl_str_mv | ALMEIDA, Rosane Mansan et al. Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations. BMC Microbiology, v. 13, Article 22, 1 fev. 2013. Disponível em: <https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22>. Acesso em: 14 jun. 2017. doi: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1186/1471-2180-13-22 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/23744 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | BMC Microbiology |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Escherichia coli Diarréia em crianças Diarréia Microorganismos Bactérias |
| dc.title.pt_BR.fl_str_mv | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Background: Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown inconsistent results. In this work, two different collections of DAEC possessing Afa/Dr genes, from children and adults, were studied regarding characteristics potentially associated to virulence. Results: DAEC strains were recovered in similar frequencies from diarrheic and asymptomatic children, and more frequently from adults with diarrhea (P < 0.01) than from asymptomatic adults. Association with diarrhea (P < 0.05) was found for SAT-positive strains recovered from children and for curli-positive strains recovered from adults. Mixed biofilms involving DAEC and a Citrobacter freundii strain have shown an improved ability to form biofilms in relation to the monocultures. Control strains have shown a greater diversity of Afa/Dr adhesins and higher frequencies of cellulose, TTSS, biofilm formation and induction of IL-8 secretion than strains from cases of diarrhea in children. Conclusions: DAEC strains possessing Afa/Dr genes isolated from children and adults represent two different bacterial populations. DAEC strains carrying genes associated to virulence can be found as part of the normal microbiota present in asymptomatic children. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_42e04e0badd1319d986c49477c8f87ea |
| identifier_str_mv | ALMEIDA, Rosane Mansan et al. Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations. BMC Microbiology, v. 13, Article 22, 1 fev. 2013. Disponível em: <https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22>. Acesso em: 14 jun. 2017. doi: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/23744 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher.none.fl_str_mv | BMC Microbiology |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Almeida, Rosane MansanPereira, Alex LeiteGiugliano, Loreny Gimenes2017-06-24T13:05:03Z2017-06-24T13:05:03Z2013-02-01ALMEIDA, Rosane Mansan et al. Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations. BMC Microbiology, v. 13, Article 22, 1 fev. 2013. Disponível em: <https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22>. Acesso em: 14 jun. 2017. doi: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-22.http://repositorio.unb.br/handle/10482/23744https://dx.doi.org/10.1186/1471-2180-13-22BMC Microbiology© 2013 Mansan-Almeida et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/openAccessDiffusely adherent Escherichia coli strains isolated from children and adults constitute two different populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEscherichia coliDiarréia em criançasDiarréiaMicroorganismosBactériasBackground: Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown inconsistent results. In this work, two different collections of DAEC possessing Afa/Dr genes, from children and adults, were studied regarding characteristics potentially associated to virulence. Results: DAEC strains were recovered in similar frequencies from diarrheic and asymptomatic children, and more frequently from adults with diarrhea (P < 0.01) than from asymptomatic adults. Association with diarrhea (P < 0.05) was found for SAT-positive strains recovered from children and for curli-positive strains recovered from adults. Mixed biofilms involving DAEC and a Citrobacter freundii strain have shown an improved ability to form biofilms in relation to the monocultures. Control strains have shown a greater diversity of Afa/Dr adhesins and higher frequencies of cellulose, TTSS, biofilm formation and induction of IL-8 secretion than strains from cases of diarrhea in children. Conclusions: DAEC strains possessing Afa/Dr genes isolated from children and adults represent two different bacterial populations. DAEC strains carrying genes associated to virulence can be found as part of the normal microbiota present in asymptomatic children.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_DiffuselyAdherentEscherichiacoli.pdfARTIGO_DiffuselyAdherentEscherichiacoli.pdfapplication/pdf1638209http://repositorio2.unb.br/jspui/bitstream/10482/23744/1/ARTIGO_DiffuselyAdherentEscherichiacoli.pdf3f50f8c9e24e4b2fbb20719cf62a5025MD51open accessLICENSElicense.txtlicense.txttext/plain160http://repositorio2.unb.br/jspui/bitstream/10482/23744/2/license.txtcd7f2de909e07c008880b5201378c22eMD52open access10482/237442023-05-24 20:34:21.347open accessoai:repositorio.unb.br:10482/23744U3VibWlzcz9vIGVmZXRpdmFkYSBwb3IgaW50ZWdyYW50ZSBkYSBlcXVpcGUgZG8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbkIgZGUgYWNvcmRvIGNvbSBsaWNlbj9hIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:21Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations Almeida, Rosane Mansan Escherichia coli Diarréia em crianças Diarréia Microorganismos Bactérias |
| status_str | publishedVersion |
| title | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| title_full | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| title_fullStr | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| title_full_unstemmed | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| title_short | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| title_sort | Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations |
| topic | Escherichia coli Diarréia em crianças Diarréia Microorganismos Bactérias |
| url | http://repositorio.unb.br/handle/10482/23744 https://dx.doi.org/10.1186/1471-2180-13-22 |