Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
I tiakina i:
| Kaituhi matua: | |
|---|---|
| Rā whakaputa: | 2004 |
| Ētahi atu kaituhi: | , , , , , , , , |
| Hōputu: | Article |
| Reo: | eng |
| Puna: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/17614 |
Whakarāpopototanga: | The objective was to determine the morphological and ultrastructural features of sheep primordial follicles preserved in either 0.9% saline solution or TCM 199 at different temperatures. Soon after death, the ovarian pair of each ewe (n=5) was divided into 25 fragments. One fragment was immediately fixed for morphological evaluation (control). The other 24 fragments were randomly distributed in tubes containing 2 ml of 0.9% saline solution or TCM 199 and maintained at 4, 20 or 39 °C for 2, 4, 12, or 24 h. Based on histological assessment, storage of ovarian fragments in 0.9% saline solution at 20 °C for up to 24 h and in both solutions at 39 °C for 4, 12 or 24 h increased (P<0.01) the percentage of degenerate primordial follicles compared with controls. In contrast, preservation at 4 °C in both solutions, kept the percentage of morphologically normal primordial follicles similar to control values. Although histological integrity of primordial follicles was maintained in fragments stored at 20 °C for up to 24 h in TCM 199, these results were not confirmed by ultrastructural analysis. Based on transmission electron microscopy, only primordial follicles stored at 4 °C for up to 24 h, at 20 °C for up to 12 h and at 39 °C for up to 2 h in both solutions were ultrastructurally normal. In conclusion, sheep primordial follicles were successfully preserved at 4 °C for up to 24 h, at 20 °C for up to 12 h and at 39 °C for 2 h in 0.9% saline solution or TCM 199. |
Ngā tūemi rite: Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
- Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro
- Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ
- Morphologic, viability and ultrastructural analysis of vitrified sheep preantral follicles enclosed in ovarian tissue
- Histological and ultrastructural analysis of cryopreserved sheep preantral follicles
- Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro
- In vitro development of primordial follicles after long-term culture of goat ovarian tissue
