Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum
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| Autor principal: | |
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| Publication Date: | 2019 |
| Outros Autores: | , , , , , , , , |
| Formato: | Article |
| Idioma: | eng |
| Fonte: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/36338 https://doi.org/10.1016/j.bjp.2019.02.001 http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |
Resumo: | Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity. |
| _version_ | 1871442232080334848 |
|---|---|
| author | Costa, Gabriella Gusmão Pereira da |
| author2 | Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
| author2_role | author author author author author author author author author |
| author_browse | Almeida, Fabrício Tavares Cunha de Costa, Gabriella Gusmão Pereira da Fagg, Christopher William Gomes, João Victor Dutra Gomes-Copeland, Kicia Karinne Pereira Santos, Izulmé Rita Imaculada Silva, Cristian Aldemar Gasca Silveira, Dâmaris Simeoni, Luiz Alberto Torres, Amanda Gomes |
| author_facet | Costa, Gabriella Gusmão Pereira da Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
| author_role | author |
| bitstream.checksum.fl_str_mv | 5b83cbc27483f1adc2fcd7088331442b |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/36338/1/ARTIGO_InfluenceVitroMicropropagation.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Costa, Gabriella Gusmão Pereira da Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
| dc.date.accessioned.fl_str_mv | 2020-01-24T10:30:21Z |
| dc.date.available.fl_str_mv | 2020-01-24T10:30:21Z |
| dc.date.issued.fl_str_mv | 2019 |
| dc.identifier.citation.fl_str_mv | COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.1016/j.bjp.2019.02.001 |
| dc.identifier.orcid.none.fl_str_mv | http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/36338 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Farmacognosia |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.none.fl_str_mv | Espectrometria de massa |
| dc.subject.keyword.pt_BR.fl_str_mv | Alcalóides Biossíntese Cromatografia a líquido Licorina |
| dc.title.pt_BR.fl_str_mv | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_1ca2eecc35b2e6c79fbd764f8e5d9e64 |
| identifier_str_mv | COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/36338 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Farmacognosia |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Costa, Gabriella Gusmão Pereira daSilva, Cristian Aldemar GascaGomes, João Victor DutraTorres, Amanda GomesSantos, Izulmé Rita ImaculadaAlmeida, Fabrício Tavares Cunha deFagg, Christopher WilliamSimeoni, Luiz AlbertoSilveira, DâmarisGomes-Copeland, Kicia Karinne Pereira2020-01-24T10:30:21Z2020-01-24T10:30:21Z2019COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020.https://repositorio.unb.br/handle/10482/36338https://doi.org/10.1016/j.bjp.2019.02.001http://orcid.org/0000-0002-3393-5609http://orcid.org/0000-0003-4392-4230http://orcid.org/0000-0001-6098-3852http://orcid.org/0000-0001-9599-4578http://orcid.org/0000-0002-1503-1497http://orcid.org/0000-0002-0531-7483http://orcid.org/0000-0001-6072-7832http://orcid.org/0000-0003-3401-290Xhttp://orcid.org/0000-0003-1851-5224http://orcid.org/0000-0003-3539-2916Sociedade Brasileira de FarmacognosiaThis is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/openAccessInfluence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAlcalóidesBiossínteseCromatografia a líquidoLicorinaEspectrometria de massaHippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_InfluenceVitroMicropropagation.pdfapplication/pdf830320http://repositorio2.unb.br/jspui/bitstream/10482/36338/1/ARTIGO_InfluenceVitroMicropropagation.pdf5b83cbc27483f1adc2fcd7088331442bMD51open access10482/363382023-05-23 21:10:22.872open accessoai:repositorio.unb.br:10482/36338Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T00:10:22Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum Costa, Gabriella Gusmão Pereira da Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa |
| status_str | publishedVersion |
| title | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| title_full | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| title_fullStr | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| title_full_unstemmed | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| title_short | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| title_sort | Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
| topic | Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa |
| url | https://repositorio.unb.br/handle/10482/36338 https://doi.org/10.1016/j.bjp.2019.02.001 http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |