The stress responsive and morphologically regulated HSP90 gene from Paracoccidioides brasiliensis is essential to cell viability
সংরক্ষণ করুন:
| প্রধান লেখক: | |
|---|---|
| প্রকাশনার তারিখ: | 2008 |
| অন্যান্য লেখক: | , , , , , , |
| বিন্যাস: | Article |
| ভাষা: | eng |
| সম্পদ: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/11824 https://dx.doi.org/10.1186/1471-2180-8-158 |
সংক্ষিপ্ত: | Background Paracoccidioides brasiliensis is a dimorphic fungus that causes the most prevalent systemic mycosis in Latin America. The response to heat shock is involved in pathogenesis, as this pathogen switches from mycelium to yeast forms in a temperature dependent fashion that is essential to establish infection. HSP90 is a molecular chaperone that helps in the folding and stabilization of selected polypeptides. HSP90 family members have been shown to present important roles in fungi, especially in the pathogenic species, as an immunodominant antigen and also as a potential antifungal therapeutic target. Results In this work, we decided to further study the Pbhsp90 gene, its expression and role in cell viability because it plays important roles in fungal physiology and pathogenesis. Thus, we have sequenced a Pbhsp90 cDNA and shown that this gene is present on the genome as a single copy. We have also confirmed its preferential expression in the yeast phase and its overexpression during dimorphic transition and oxidative stress. Treatment of the yeast with the specific HSP90 inhibitors geldanamycin and radicicol inhibited growth at 2 and 10 μM, respectively. Conclusion The data confirm that the Pbhsp90 gene encodes a morphologically regulated and stress-responsive protein whose function is essential to cell viability of this pathogen. This work also enforces the potential of HSP90 as a target for antifungal therapies, since the use of HSP90 inhibitors is lethal to the P. brasiliensis yeast cells in a dose-responsive manner. |
অনুরূপ উপাদানগুলি: The stress responsive and morphologically regulated HSP90 gene from Paracoccidioides brasiliensis is essential to cell viability
- Transcriptional profiles of the human pathogenic fungus paracoccidioides brasiliensis in mycelium and yeast cells
- Análise estrutural e funcional da região promotora do gene quitina sintase 4 de Paracoccidioides brasiliensis
- Proteinas de superficie de Paracoccidioides brasiliensis
- Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction
- Análise da região promotora dos genes 1,3-ß-glicana sintase e quitina sintase 4 de Paracoccidioides brasiliensis
- Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential