In vitro evaluation of NLS-DTX activity in triple-negative breast cancer
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| Autor principal: | |
|---|---|
| Data de publicació: | 2022 |
| Altres autors: | , |
| Format: | Article |
| Idioma: | eng |
| Font: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/44766 https://doi.org/10.3390/molecules27154920 https://orcid.org/0000-0001-9149-316X https://orcid.org/0000-0002-9873-3098 |
Sumari: | Cancer is one of the most lethal diseases in the world, and the development and improvement of treatments used in cancer therapies are extremely important for a better quality of life for patients. In view of the current problems in drug administration such as low solubility and adverse effects, the activity of a solid lipid nanoparticle containing docetaxel (SLN-DTX), a drug already used in conventional therapies, was evaluated in a cell line (MDA-MB-231) of one of the most aggressive types of breast cancer with the worst prognosis, triple-negative breast cancer. Viability tests indicated that SLN-DTX has a greater dependence on the treatment dose when compared to the free drug, which indicates a more controlled release of the drug, and both reduced viability by around 50% at a concentration of 1 µg/mL after 72 h. Transmission electron microscopy (TEM) and confocal and light microscopy analyses indicated that after treatment the cells enter a mitotic catastrophe, characteristic of antimitotic drugs that usually make cells progress to death or senescence. Cells treated with both DTX and SLN-DTX showed significant inhibition of mobility, 73.6% and 66.5% when treated with SLN-DTX and DTX, respectively, compared to the 11.4% of the control after 72 h, characteristics that are very relevant in tumor development and progression. SLN-DTX demonstrated its great potential as a nanocarrier by maintaining and improving the drug’s action in the MDA-MB-231 cell line. |
| _version_ | 1871442203731034112 |
|---|---|
| author | Paiva, Karen Letycia Rodrigues de |
| author2 | Radicchi, Marina A. Báo, Sônia Nair |
| author2_role | author author |
| author_browse | Báo, Sônia Nair Paiva, Karen Letycia Rodrigues de Radicchi, Marina A. |
| author_facet | Paiva, Karen Letycia Rodrigues de Radicchi, Marina A. Báo, Sônia Nair |
| author_role | author |
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| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Paiva, Karen Letycia Rodrigues de Radicchi, Marina A. Báo, Sônia Nair |
| dc.contributor.email.pt_BR.fl_str_mv | mailto:karendepaiva@gmail.com mailto:maradicchi.bep@gmail.com mailto:snbao@unb.br |
| dc.date.accessioned.fl_str_mv | 2022-09-12T18:06:58Z |
| dc.date.available.fl_str_mv | 2022-09-12T18:06:58Z |
| dc.date.issued.fl_str_mv | 2022-08-02 |
| dc.identifier.citation.fl_str_mv | PAIVA, Karen L. R.; RADICCHI, Marina A.; BÁO, Sônia N. In vitro evaluation of NLS-DTX activity in triple-negative breast cancer. Molecules , Basel, v. 27, n. 15, art. 4920, 2022. DOI 10.3390/molecules27154920. Disponível em: https://www.mdpi.com/1420-3049/27/15/4920. Acesso em: 12 set. 2022. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/molecules27154920 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0001-9149-316X https://orcid.org/0000-0002-9873-3098 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/44766 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Nanopartículas lipídicas sólidas Nanopartículas Câncer de mama triplo negativo Docetaxel Mamas - câncer |
| dc.title.pt_BR.fl_str_mv | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Cancer is one of the most lethal diseases in the world, and the development and improvement of treatments used in cancer therapies are extremely important for a better quality of life for patients. In view of the current problems in drug administration such as low solubility and adverse effects, the activity of a solid lipid nanoparticle containing docetaxel (SLN-DTX), a drug already used in conventional therapies, was evaluated in a cell line (MDA-MB-231) of one of the most aggressive types of breast cancer with the worst prognosis, triple-negative breast cancer. Viability tests indicated that SLN-DTX has a greater dependence on the treatment dose when compared to the free drug, which indicates a more controlled release of the drug, and both reduced viability by around 50% at a concentration of 1 µg/mL after 72 h. Transmission electron microscopy (TEM) and confocal and light microscopy analyses indicated that after treatment the cells enter a mitotic catastrophe, characteristic of antimitotic drugs that usually make cells progress to death or senescence. Cells treated with both DTX and SLN-DTX showed significant inhibition of mobility, 73.6% and 66.5% when treated with SLN-DTX and DTX, respectively, compared to the 11.4% of the control after 72 h, characteristics that are very relevant in tumor development and progression. SLN-DTX demonstrated its great potential as a nanocarrier by maintaining and improving the drug’s action in the MDA-MB-231 cell line. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_05b32fc4e1562fcd44cce286df2886e1 |
| identifier_str_mv | PAIVA, Karen L. R.; RADICCHI, Marina A.; BÁO, Sônia N. In vitro evaluation of NLS-DTX activity in triple-negative breast cancer. Molecules , Basel, v. 27, n. 15, art. 4920, 2022. DOI 10.3390/molecules27154920. Disponível em: https://www.mdpi.com/1420-3049/27/15/4920. Acesso em: 12 set. 2022. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/44766 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
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| spelling | Paiva, Karen Letycia Rodrigues deRadicchi, Marina A.Báo, Sônia Nairmailto:karendepaiva@gmail.commailto:maradicchi.bep@gmail.commailto:snbao@unb.br2022-09-12T18:06:58Z2022-09-12T18:06:58Z2022-08-02PAIVA, Karen L. R.; RADICCHI, Marina A.; BÁO, Sônia N. In vitro evaluation of NLS-DTX activity in triple-negative breast cancer. Molecules , Basel, v. 27, n. 15, art. 4920, 2022. DOI 10.3390/molecules27154920. Disponível em: https://www.mdpi.com/1420-3049/27/15/4920. Acesso em: 12 set. 2022.https://repositorio.unb.br/handle/10482/44766https://doi.org/10.3390/molecules27154920https://orcid.org/0000-0001-9149-316Xhttps://orcid.org/0000-0002-9873-3098MDPIMolecules - No special permission is required to reuse all or part of article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. Reuse of an article does not imply endorsement by the authors or MDPI. Fonte: https://www.mdpi.com/openaccess. Acesso em: 12 set. 2022.info:eu-repo/semantics/openAccessIn vitro evaluation of NLS-DTX activity in triple-negative breast cancerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNanopartículas lipídicas sólidasNanopartículasCâncer de mama triplo negativoDocetaxelMamas - câncerCancer is one of the most lethal diseases in the world, and the development and improvement of treatments used in cancer therapies are extremely important for a better quality of life for patients. In view of the current problems in drug administration such as low solubility and adverse effects, the activity of a solid lipid nanoparticle containing docetaxel (SLN-DTX), a drug already used in conventional therapies, was evaluated in a cell line (MDA-MB-231) of one of the most aggressive types of breast cancer with the worst prognosis, triple-negative breast cancer. Viability tests indicated that SLN-DTX has a greater dependence on the treatment dose when compared to the free drug, which indicates a more controlled release of the drug, and both reduced viability by around 50% at a concentration of 1 µg/mL after 72 h. Transmission electron microscopy (TEM) and confocal and light microscopy analyses indicated that after treatment the cells enter a mitotic catastrophe, characteristic of antimitotic drugs that usually make cells progress to death or senescence. Cells treated with both DTX and SLN-DTX showed significant inhibition of mobility, 73.6% and 66.5% when treated with SLN-DTX and DTX, respectively, compared to the 11.4% of the control after 72 h, characteristics that are very relevant in tumor development and progression. SLN-DTX demonstrated its great potential as a nanocarrier by maintaining and improving the drug’s action in the MDA-MB-231 cell line.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBLICENSElicense.txtlicense.txttext/plain163http://repositorio2.unb.br/jspui/bitstream/10482/44766/2/license.txtba54f8d1c5f5ec8df897ad678916c701MD52open accessORIGINALARTIGO_InVitroEvaluation.pdfARTIGO_InVitroEvaluation.pdfapplication/pdf4571799http://repositorio2.unb.br/jspui/bitstream/10482/44766/1/ARTIGO_InVitroEvaluation.pdf42bc2a7d0a9027d74147a6df424da375MD51open access10482/447662023-05-24 20:34:33.953open accessoai:repositorio.unb.br:10482/44766U3VibWlzc8OjbyBlZmV0aXZhZGEgcG9yIGludGVncmFudGUgZGEgZXF1aXBlIGRvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuQiBkZSBhY29yZG8gY29tIGxpY2Vuw6dhIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:33Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer Paiva, Karen Letycia Rodrigues de Nanopartículas lipídicas sólidas Nanopartículas Câncer de mama triplo negativo Docetaxel Mamas - câncer |
| status_str | publishedVersion |
| title | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| title_full | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| title_fullStr | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| title_full_unstemmed | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| title_short | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| title_sort | In vitro evaluation of NLS-DTX activity in triple-negative breast cancer |
| topic | Nanopartículas lipídicas sólidas Nanopartículas Câncer de mama triplo negativo Docetaxel Mamas - câncer |
| url | https://repositorio.unb.br/handle/10482/44766 https://doi.org/10.3390/molecules27154920 https://orcid.org/0000-0001-9149-316X https://orcid.org/0000-0002-9873-3098 |