The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species
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| Hlavní autor: | |
|---|---|
| Datum vydání: | 2001 |
| Další autoři: | , , |
| Médium: | Article |
| Jazyk: | eng |
| Zdroj: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/11814 http://dx.doi.org/10.1590/S0100-879X2001001100018 |
Shrnutí: | Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (•OH, •O2-, H2O2, 1O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 109 M-1 s-1 for KA and 1.0 x 109 M-1 s-1 for PL, similar to that of ethanol (1.4 x 109 M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited •O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of 1O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an antioxidant effect, scavenging three of four reactive oxygen species tested here. Unlike KA, PL did not significantly scavenge hydrogen peroxide. |
| _version_ | 1871442197757296640 |
|---|---|
| author | Gomes, Anderson de Jesus |
| author2 | Lunardi, Claure Nain Gonzalez, Salvador Tedesco, Antonio Claudio |
| author2_role | author author author |
| author_browse | Gomes, Anderson de Jesus Gonzalez, Salvador Lunardi, Claure Nain Tedesco, Antonio Claudio |
| author_facet | Gomes, Anderson de Jesus Lunardi, Claure Nain Gonzalez, Salvador Tedesco, Antonio Claudio |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/11814/1/ARTIGO_AntioxidantActionPolypodium.PDF http://repositorio2.unb.br/jspui/bitstream/10482/11814/2/license.txt http://repositorio2.unb.br/jspui/bitstream/10482/11814/3/ARTIGO_AntioxidantActionPolypodium.PDF.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Gomes, Anderson de Jesus Lunardi, Claure Nain Gonzalez, Salvador Tedesco, Antonio Claudio |
| dc.date.accessioned.fl_str_mv | 2012-12-14T19:26:53Z |
| dc.date.available.fl_str_mv | 2012-12-14T19:26:53Z |
| dc.date.issued.fl_str_mv | 2001-11 |
| dc.identifier.citation.fl_str_mv | GOMES, A.J. et al. The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 34, n. 11, nov. 2001. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018&lng=pt&nrm=iso>. Acesso em: 8 nov. 2012. http://dx.doi.org/10.1590/S0100-879X2001001100018. |
| dc.identifier.doi.en.fl_str_mv | http://dx.doi.org/10.1590/S0100-879X2001001100018 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/11814 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.en.fl_str_mv | Polipódio Antioxidantes Radicais livres (Química) Ácido kójico |
| dc.title.en.fl_str_mv | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (•OH, •O2-, H2O2, 1O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 109 M-1 s-1 for KA and 1.0 x 109 M-1 s-1 for PL, similar to that of ethanol (1.4 x 109 M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited •O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of 1O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an antioxidant effect, scavenging three of four reactive oxygen species tested here. Unlike KA, PL did not significantly scavenge hydrogen peroxide. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_0072dc2f7edd7b5de7ca0f75a968f71d |
| identifier_str_mv | GOMES, A.J. et al. The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 34, n. 11, nov. 2001. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018&lng=pt&nrm=iso>. Acesso em: 8 nov. 2012. http://dx.doi.org/10.1590/S0100-879X2001001100018. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/11814 |
| publishDate | 2001 |
| publishDateSort | 2001 |
| publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Gomes, Anderson de JesusLunardi, Claure NainGonzalez, SalvadorTedesco, Antonio Claudio2012-12-14T19:26:53Z2012-12-14T19:26:53Z2001-11GOMES, A.J. et al. The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 34, n. 11, nov. 2001. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018&lng=pt&nrm=iso>. Acesso em: 8 nov. 2012. http://dx.doi.org/10.1590/S0100-879X2001001100018.http://repositorio.unb.br/handle/10482/11814http://dx.doi.org/10.1590/S0100-879X2001001100018Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (•OH, •O2-, H2O2, 1O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 109 M-1 s-1 for KA and 1.0 x 109 M-1 s-1 for PL, similar to that of ethanol (1.4 x 109 M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited •O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of 1O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an antioxidant effect, scavenging three of four reactive oxygen species tested here. Unlike KA, PL did not significantly scavenge hydrogen peroxide.Associação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-879X&lng=pt&nrm=iso. Acesso em: 8 nov. 2012.info:eu-repo/semantics/openAccessThe antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePolipódioAntioxidantesRadicais livres (Química)Ácido kójicoengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_AntioxidantActionPolypodium.PDFARTIGO_AntioxidantActionPolypodium.PDFapplication/pdf69701http://repositorio2.unb.br/jspui/bitstream/10482/11814/1/ARTIGO_AntioxidantActionPolypodium.PDFb413e862ce35388fb574403c6e618e19MD51open accessLICENSElicense.txtlicense.txttext/plain265http://repositorio2.unb.br/jspui/bitstream/10482/11814/2/license.txt5515065ed33a1085a568e49e85ff116dMD52open accessTEXTARTIGO_AntioxidantActionPolypodium.PDF.txtARTIGO_AntioxidantActionPolypodium.PDF.txtExtracted texttext/plain27222http://repositorio2.unb.br/jspui/bitstream/10482/11814/3/ARTIGO_AntioxidantActionPolypodium.PDF.txtc6361cc7afd96796a712014e68502aa8MD53open access10482/118142023-05-19 21:51:06.334open accessoai:repositorio.unb.br:10482/11814TGljZW5zZSBncmFudGVkIGJ5IFNhYnJpbmEgU2lsdmEgZGUgTWFjZWRvIChzYWJyaW5hbWFjZWRvQGJjZS51bmIuYnIpIG9uIDIwMTItMTEtMDhUMTM6MTc6NTRaIChHTVQpOgoKU3VibWlzc8OjbyBlZmV0aXZhZGEgcG9yIGludGVncmFudGUgZGEgZXF1aXBlIGRvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuQiBkZSBhY29yZG8gY29tIGxpY2Vuw6dhIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-20T00:51:06Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species Gomes, Anderson de Jesus Polipódio Antioxidantes Radicais livres (Química) Ácido kójico |
| status_str | publishedVersion |
| title | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| title_full | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| title_fullStr | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| title_full_unstemmed | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| title_short | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| title_sort | The antioxidant action of Polypodium leucotomos extract and kojic acid : reactions with reactive oxygen species |
| topic | Polipódio Antioxidantes Radicais livres (Química) Ácido kójico |
| url | http://repositorio.unb.br/handle/10482/11814 http://dx.doi.org/10.1590/S0100-879X2001001100018 |