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Prevalência e caracterização molecular de bastonetes Gram negativos isolados do sistema de transporte público coletivo do município de Goiânia-Go

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Xehetasun bibliografikoak
Egile nagusia: Lamaro, Luana
Argitaratze data: 2017
Formatua: Master thesis
Hizkuntza: por
Baliabidea: Repositório Institucional da UFG
Download full: http://repositorio.bc.ufg.br/tede/handle/tede/7948
Gaia: Objects contaminated with microorganisms are denominated fomites. A number of studies have reported fomites as sources of microorganism dissemination. This study aimed to isolate and identify Gram Negative Bacilli (GNB) from a collective public transport line in the city of Goiânia, GO, to characterize by phenotypic and genotypic methods in the antimicrobial susceptibility profile and to analyze the macrorrestricity profile of the isolated. As samples of the fixed bars surfaces of the buses and ratchets of the platforms and terminals of “Leste-Oeste” line were collected with swab and sent to the Laboratory of Applied Bacteriology of the IPTSP-UFG. After the GNB culture, as species were identified by PCR and submitted to the Antimicrobial Susceptibility Test (AST). The plasmid DNA of the strains resistant to beta-lactams was extracted for the search of genes encoding beta-lactamases by PCR. The macrorrestriction profile of P. mirabilis strains was performed by PFGE using the XbaI enzyme. A total of 852 swabs were collected representing a prevalence of 46.0% of GNB in the evaluated surfaces. The species identified were A. baumannii (40.8%), H. alvei (30.9%), P. mirabilis (26.1%) and E. coli (2.2%). The AST revealed 31 (7.9%) multidrug resistant isolates. The blaTEM and blaCTX genes were detected. We observed a presence of eight clusters and three clones among the species P. mirabilis. This study proves that the surfaces present in the collective public transport system can be sources of resistant multi-drug GNB and these bacteria have the potential of dispersion and permanence in different community environments.
Gaia:Objects contaminated with microorganisms are denominated fomites. A number of studies have reported fomites as sources of microorganism dissemination. This study aimed to isolate and identify Gram Negative Bacilli (GNB) from a collective public transport line in the city of Goiânia, GO, to characterize by phenotypic and genotypic methods in the antimicrobial susceptibility profile and to analyze the macrorrestricity profile of the isolated. As samples of the fixed bars surfaces of the buses and ratchets of the platforms and terminals of “Leste-Oeste” line were collected with swab and sent to the Laboratory of Applied Bacteriology of the IPTSP-UFG. After the GNB culture, as species were identified by PCR and submitted to the Antimicrobial Susceptibility Test (AST). The plasmid DNA of the strains resistant to beta-lactams was extracted for the search of genes encoding beta-lactamases by PCR. The macrorrestriction profile of P. mirabilis strains was performed by PFGE using the XbaI enzyme. A total of 852 swabs were collected representing a prevalence of 46.0% of GNB in the evaluated surfaces. The species identified were A. baumannii (40.8%), H. alvei (30.9%), P. mirabilis (26.1%) and E. coli (2.2%). The AST revealed 31 (7.9%) multidrug resistant isolates. The blaTEM and blaCTX genes were detected. We observed a presence of eight clusters and three clones among the species P. mirabilis. This study proves that the surfaces present in the collective public transport system can be sources of resistant multi-drug GNB and these bacteria have the potential of dispersion and permanence in different community environments.

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