Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos
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| Data de publicació: | 2012 |
| Format: | Doctoral thesis |
| Idioma: | por |
| Font: | Repositório Institucional da UFG |
| Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/3310 |
Sumari: | This study was divided in chapters that include various aspects of the interaction between the entomopathogenic fungus M. anisopliae with rice stink bug T. limbativentris as follows: Chapter 1 (Theoretical considerations) describes an introduction about the elements involved in this study as: the importance of rice crops for Brazil, as well as socio-economic and planted areas with this crop. It also covers information about life cycle and ecology of the rice stink bug, Tibraca limbativentris stal (1869) along with its importance as a pest. Chemical and biological control measures are discussed as well. Regarding the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin, general aspects of taxonomy, morphology, infection process of this fungus to their hosts and virulence factors were also addressed. To sum up, chemical ecology was explored addressing intraspecific and interspecific interactions among insect family pentatomidae with emphasis on studies of chemical compounds of defense in order to understand the natural resistance of this group of insects to entomopathogenic fungi. Chapter 2 (Toxicity of compounds extracted from the cuticle and the stink methatoracic gland (MTG) of T. limbativentris to the fungus M. anisopliae) describes the susceptibility of different life stages of T. limbativentris to M. anisopliae and correlates these results with the fungicidal activity of aldehydes (E)-2-octenal and (E)-2-dedecenal extracted from metathoracic scent glands (MTG) of adults as well as the (E)-2-hexenal, extracted from dorsal abdominal scent glands (DAG) of nymphal stages (second to fifth instar), by collecting exuviae. All compounds extracted were characterized by GC-MS and their fungistatic potential determined measuring the minimum inhibitory concentration (MIC) of these compounds, which expresses the ability of these compounds to inhibit the conidial germination of this fungus entirely. The aldehyde (E)-2-decenal was the most harmful to M. anisopliae germination, with MIC of 0.003% (30 ppm) compared to the (E)-2-hexenal and (E)-2-octenal, which in turn showed MIC of 0.012 and 0.025%, respectively. Chapter 3 (Effect of pesticides used in conventional rice cultivation with the entomopathogenic fungus M. anisopliae.) Describes the assessment of the compatibility of M. anisopliae to eight insecticides, five herbicides and four fungicides registered for rice cultivation. In this study we evaluated the germination (GER), vegetative growth (VG) and sporulation (ESP) of the fungus after three hours in the mixture, stirring with agrochemicals. The parameters evaluated were the fungus equated using the following formula: BI = [(VG * 47) + (ESP * 43) + (10 * GER)] / 100, in which through the biological index value (BI) was possible to determine the compatibility level of agrochemicals to the fungus. Among the tested pesticides, insecticides Bravic™, Actara™, Karate Zeon™; herbicide Roundup™, Basagran™, Kifix™ and fungicide Priori ™ were the only products compatible with M. anisopliae. Chapter 4 (enhanced pathogenicity toT. limbativentris with sublethal doses of chemical insecticides.) describes the interaction of M. anisopliae combined with sublethal concentrations of thiamethoxam against adults of T. limbativentris. This combination as hypothesis has to overcome the natural chemical resistance of T. limbativentris to M. anisopliae. The results showed that T. limbativentris was more susceptible to M. anisopliae (strain CG-168) associated with sublethal doses of thiamethoxam (Actara™) or lambda-cyhalothrin (Karate Zeon™) than the fungus alone. In addition, the optimum concentration was observed for the fungus at 5x107 conidia combined with concentrations of the insecticide thiamethoxam. The fungus in xxi this concentration mixed with thiamethoxam at 3.1 ppm caused 80% adult mortality, whereas the mortality rates in treatments with the fungus and 3.1 ppm thiamethoxam both applied alone were 38 and 35% respectively. Sublethal doses of Actara™ (3.1 ppm) and Karate Zeon™ (186 ppm) was estimated by Probit equation. For the purpose of using a commercial strain of M. anisopliae, the virulence of strain CG-168, used in all previous tests, was compared to strain 1037-ESALQ, registered commercially. According to the results both strains had similar virulence to adult T. limbativentris. commercially registered in Brazil market. According to the results, adults of T. Limbativentris were similarly susceptible to both strains of M. anisopliae. In the field experiment, five treatments were prepared: Fungus + Actara™ sublethal dose (50g ha), Fungus, Actara™ sublethal dose (50g./ha) Actara™ full dose (200g./ha) and control; which were applied on rice plants infested with insects inside nylon cages under field conditions. After seven and 14 days of application, 20 insects were collected from each cage and evaluated in the laboratory. According to the results, the evaluation of the first collection, mixture of M. anisopliae with 50g/ha Actara™ caused 54% mortality compared with 40, 32, 26 and 18% respectively for the full dose of Actara™ (200g/ha), fungus alone, Actara™ sublethal dose (50g/ha) and control. In the second collection, fungus + Actara™ highlightedin relation to the others, causing a 50% mortality, while treatment with the fungus alone the mortality was16% and the others treatment was 0% As a result, we found a synergism effect between fungus and sublethal dose of the insecticide. In summary, our results highlight the potential of sublethal concentrations of the neonicotinoid insecticide thiamethoxan in increasing the susceptibility of adults of T. Limbativentris to the fungus M. anisopliae. In fact, this strategy consists in a control method effective and feasible to combat this stink rice bug in the field from a viewpoint of an integrated pest management and sustainability. |
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| author | Silva , Rodrigo Alves da |
| author_browse | Silva , Rodrigo Alves da |
| author_facet | Silva , Rodrigo Alves da |
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| collection | Repositório Institucional da UFG |
| contributor_str_mv | Lião , Luciano Morais Lião, Luciano Morais Ferri, Pedro Henrique Fernandes, João Batista Barrigossi, José Alexandre Laumann, Raúl Alberto |
| dc.contributor.advisor1.fl_str_mv | Lião , Luciano Morais |
| dc.contributor.advisor1Lattes.fl_str_mv | http://lattes.cnpq.br/2647529909397336 |
| dc.contributor.author.fl_str_mv | Silva , Rodrigo Alves da |
| dc.contributor.authorLattes.fl_str_mv | http://lattes.cnpq.br/7191202638605415 |
| dc.contributor.referee1.fl_str_mv | Lião, Luciano Morais |
| dc.contributor.referee2.fl_str_mv | Ferri, Pedro Henrique |
| dc.contributor.referee3.fl_str_mv | Fernandes, João Batista |
| dc.contributor.referee4.fl_str_mv | Barrigossi, José Alexandre |
| dc.contributor.referee5.fl_str_mv | Laumann, Raúl Alberto |
| dc.date.accessioned.fl_str_mv | 2014-10-09T14:40:07Z |
| dc.date.issued.fl_str_mv | 2012-09-28 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.citation.fl_str_mv | SILVA, Rodrigo Alves da. Estudo de Metarhizium anisopliae (Metsch) Sorok: toxicidade a compostos extraídos de Tibraca limbativentris Stal (Heteroptera: Pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos. 2012. 127 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2012. |
| dc.identifier.uri.fl_str_mv | http://repositorio.bc.ufg.br/tede/handle/tede/3310 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.country.fl_str_mv | Brasil |
| dc.publisher.department.fl_str_mv | Instituto de Química - IQ (RG) |
| dc.publisher.initials.fl_str_mv | UFG |
| dc.publisher.none.fl_str_mv | Universidade Federal de Goiás |
| dc.publisher.program.fl_str_mv | Programa de Pós-graduação em Química (IQ) |
| dc.relation.cnpq.fl_str_mv | -8194069717282802154 |
| dc.relation.confidence.fl_str_mv | 600 600 600 |
| dc.relation.department.fl_str_mv | 7826066743741197278 |
| dc.relation.program.fl_str_mv | 663693921325415158 |
| dc.rights.driver.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
| dc.subject.cnpq.fl_str_mv | QUIMICA::QUIMICA ORGANICA |
| dc.subject.eng.fl_str_mv | Biological control Entomophatogenic fungi Sustainability |
| dc.subject.por.fl_str_mv | Controle biológico Fungo entomopatogênico Sustemtabilidade |
| dc.title.alternative.eng.fl_str_mv | Study of metarhizium anisopliae (metsch) sorok: toxicity of the compounds extracted from tibraca limbativentris stal (heteroptera: pentatomidae), effects of agrochemicals used in rice crops and enhanced pathogenicity to T. limbativentris with sublethal doses of chemical insecticides |
| dc.title.por.fl_str_mv | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/doctoralThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | This study was divided in chapters that include various aspects of the interaction between the entomopathogenic fungus M. anisopliae with rice stink bug T. limbativentris as follows: Chapter 1 (Theoretical considerations) describes an introduction about the elements involved in this study as: the importance of rice crops for Brazil, as well as socio-economic and planted areas with this crop. It also covers information about life cycle and ecology of the rice stink bug, Tibraca limbativentris stal (1869) along with its importance as a pest. Chemical and biological control measures are discussed as well. Regarding the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin, general aspects of taxonomy, morphology, infection process of this fungus to their hosts and virulence factors were also addressed. To sum up, chemical ecology was explored addressing intraspecific and interspecific interactions among insect family pentatomidae with emphasis on studies of chemical compounds of defense in order to understand the natural resistance of this group of insects to entomopathogenic fungi. Chapter 2 (Toxicity of compounds extracted from the cuticle and the stink methatoracic gland (MTG) of T. limbativentris to the fungus M. anisopliae) describes the susceptibility of different life stages of T. limbativentris to M. anisopliae and correlates these results with the fungicidal activity of aldehydes (E)-2-octenal and (E)-2-dedecenal extracted from metathoracic scent glands (MTG) of adults as well as the (E)-2-hexenal, extracted from dorsal abdominal scent glands (DAG) of nymphal stages (second to fifth instar), by collecting exuviae. All compounds extracted were characterized by GC-MS and their fungistatic potential determined measuring the minimum inhibitory concentration (MIC) of these compounds, which expresses the ability of these compounds to inhibit the conidial germination of this fungus entirely. The aldehyde (E)-2-decenal was the most harmful to M. anisopliae germination, with MIC of 0.003% (30 ppm) compared to the (E)-2-hexenal and (E)-2-octenal, which in turn showed MIC of 0.012 and 0.025%, respectively. Chapter 3 (Effect of pesticides used in conventional rice cultivation with the entomopathogenic fungus M. anisopliae.) Describes the assessment of the compatibility of M. anisopliae to eight insecticides, five herbicides and four fungicides registered for rice cultivation. In this study we evaluated the germination (GER), vegetative growth (VG) and sporulation (ESP) of the fungus after three hours in the mixture, stirring with agrochemicals. The parameters evaluated were the fungus equated using the following formula: BI = [(VG * 47) + (ESP * 43) + (10 * GER)] / 100, in which through the biological index value (BI) was possible to determine the compatibility level of agrochemicals to the fungus. Among the tested pesticides, insecticides Bravic™, Actara™, Karate Zeon™; herbicide Roundup™, Basagran™, Kifix™ and fungicide Priori ™ were the only products compatible with M. anisopliae. Chapter 4 (enhanced pathogenicity toT. limbativentris with sublethal doses of chemical insecticides.) describes the interaction of M. anisopliae combined with sublethal concentrations of thiamethoxam against adults of T. limbativentris. This combination as hypothesis has to overcome the natural chemical resistance of T. limbativentris to M. anisopliae. The results showed that T. limbativentris was more susceptible to M. anisopliae (strain CG-168) associated with sublethal doses of thiamethoxam (Actara™) or lambda-cyhalothrin (Karate Zeon™) than the fungus alone. In addition, the optimum concentration was observed for the fungus at 5x107 conidia combined with concentrations of the insecticide thiamethoxam. The fungus in xxi this concentration mixed with thiamethoxam at 3.1 ppm caused 80% adult mortality, whereas the mortality rates in treatments with the fungus and 3.1 ppm thiamethoxam both applied alone were 38 and 35% respectively. Sublethal doses of Actara™ (3.1 ppm) and Karate Zeon™ (186 ppm) was estimated by Probit equation. For the purpose of using a commercial strain of M. anisopliae, the virulence of strain CG-168, used in all previous tests, was compared to strain 1037-ESALQ, registered commercially. According to the results both strains had similar virulence to adult T. limbativentris. commercially registered in Brazil market. According to the results, adults of T. Limbativentris were similarly susceptible to both strains of M. anisopliae. In the field experiment, five treatments were prepared: Fungus + Actara™ sublethal dose (50g ha), Fungus, Actara™ sublethal dose (50g./ha) Actara™ full dose (200g./ha) and control; which were applied on rice plants infested with insects inside nylon cages under field conditions. After seven and 14 days of application, 20 insects were collected from each cage and evaluated in the laboratory. According to the results, the evaluation of the first collection, mixture of M. anisopliae with 50g/ha Actara™ caused 54% mortality compared with 40, 32, 26 and 18% respectively for the full dose of Actara™ (200g/ha), fungus alone, Actara™ sublethal dose (50g/ha) and control. In the second collection, fungus + Actara™ highlightedin relation to the others, causing a 50% mortality, while treatment with the fungus alone the mortality was16% and the others treatment was 0% As a result, we found a synergism effect between fungus and sublethal dose of the insecticide. In summary, our results highlight the potential of sublethal concentrations of the neonicotinoid insecticide thiamethoxan in increasing the susceptibility of adults of T. Limbativentris to the fungus M. anisopliae. In fact, this strategy consists in a control method effective and feasible to combat this stink rice bug in the field from a viewpoint of an integrated pest management and sustainability. |
| eu_rights_str_mv | openAccess |
| format | doctoralThesis |
| id | UFG-2_f3f92e5e432e8b4a0f8a1daefd04a2f8 |
| identifier_str_mv | SILVA, Rodrigo Alves da. Estudo de Metarhizium anisopliae (Metsch) Sorok: toxicidade a compostos extraídos de Tibraca limbativentris Stal (Heteroptera: Pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos. 2012. 127 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2012. |
| instacron_str | UFG |
| institution | UFG |
| instname_str | Universidade Federal de Goiás (UFG) |
| language | por |
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| oai_identifier_str | oai:repositorio.bc.ufg.br:tede/3310 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher.none.fl_str_mv | Universidade Federal de Goiás |
| reponame_str | Repositório Institucional da UFG |
| repository.mail.fl_str_mv | grt.bc@ufg.br |
| repository.name.fl_str_mv | Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
| repository_id_str | oai:repositorio.bc.ufg.br:tede/1234 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Lião , Luciano Moraishttp://lattes.cnpq.br/2647529909397336Lião, Luciano MoraisFerri, Pedro HenriqueFernandes, João BatistaBarrigossi, José AlexandreLaumann, Raúl Albertohttp://lattes.cnpq.br/7191202638605415Silva , Rodrigo Alves da2014-10-09T14:40:07Z2012-09-28SILVA, Rodrigo Alves da. Estudo de Metarhizium anisopliae (Metsch) Sorok: toxicidade a compostos extraídos de Tibraca limbativentris Stal (Heteroptera: Pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos. 2012. 127 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tede/3310This study was divided in chapters that include various aspects of the interaction between the entomopathogenic fungus M. anisopliae with rice stink bug T. limbativentris as follows: Chapter 1 (Theoretical considerations) describes an introduction about the elements involved in this study as: the importance of rice crops for Brazil, as well as socio-economic and planted areas with this crop. It also covers information about life cycle and ecology of the rice stink bug, Tibraca limbativentris stal (1869) along with its importance as a pest. Chemical and biological control measures are discussed as well. Regarding the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin, general aspects of taxonomy, morphology, infection process of this fungus to their hosts and virulence factors were also addressed. To sum up, chemical ecology was explored addressing intraspecific and interspecific interactions among insect family pentatomidae with emphasis on studies of chemical compounds of defense in order to understand the natural resistance of this group of insects to entomopathogenic fungi. Chapter 2 (Toxicity of compounds extracted from the cuticle and the stink methatoracic gland (MTG) of T. limbativentris to the fungus M. anisopliae) describes the susceptibility of different life stages of T. limbativentris to M. anisopliae and correlates these results with the fungicidal activity of aldehydes (E)-2-octenal and (E)-2-dedecenal extracted from metathoracic scent glands (MTG) of adults as well as the (E)-2-hexenal, extracted from dorsal abdominal scent glands (DAG) of nymphal stages (second to fifth instar), by collecting exuviae. All compounds extracted were characterized by GC-MS and their fungistatic potential determined measuring the minimum inhibitory concentration (MIC) of these compounds, which expresses the ability of these compounds to inhibit the conidial germination of this fungus entirely. The aldehyde (E)-2-decenal was the most harmful to M. anisopliae germination, with MIC of 0.003% (30 ppm) compared to the (E)-2-hexenal and (E)-2-octenal, which in turn showed MIC of 0.012 and 0.025%, respectively. Chapter 3 (Effect of pesticides used in conventional rice cultivation with the entomopathogenic fungus M. anisopliae.) Describes the assessment of the compatibility of M. anisopliae to eight insecticides, five herbicides and four fungicides registered for rice cultivation. In this study we evaluated the germination (GER), vegetative growth (VG) and sporulation (ESP) of the fungus after three hours in the mixture, stirring with agrochemicals. The parameters evaluated were the fungus equated using the following formula: BI = [(VG * 47) + (ESP * 43) + (10 * GER)] / 100, in which through the biological index value (BI) was possible to determine the compatibility level of agrochemicals to the fungus. Among the tested pesticides, insecticides Bravic™, Actara™, Karate Zeon™; herbicide Roundup™, Basagran™, Kifix™ and fungicide Priori ™ were the only products compatible with M. anisopliae. Chapter 4 (enhanced pathogenicity toT. limbativentris with sublethal doses of chemical insecticides.) describes the interaction of M. anisopliae combined with sublethal concentrations of thiamethoxam against adults of T. limbativentris. This combination as hypothesis has to overcome the natural chemical resistance of T. limbativentris to M. anisopliae. The results showed that T. limbativentris was more susceptible to M. anisopliae (strain CG-168) associated with sublethal doses of thiamethoxam (Actara™) or lambda-cyhalothrin (Karate Zeon™) than the fungus alone. In addition, the optimum concentration was observed for the fungus at 5x107 conidia combined with concentrations of the insecticide thiamethoxam. The fungus in xxi this concentration mixed with thiamethoxam at 3.1 ppm caused 80% adult mortality, whereas the mortality rates in treatments with the fungus and 3.1 ppm thiamethoxam both applied alone were 38 and 35% respectively. Sublethal doses of Actara™ (3.1 ppm) and Karate Zeon™ (186 ppm) was estimated by Probit equation. For the purpose of using a commercial strain of M. anisopliae, the virulence of strain CG-168, used in all previous tests, was compared to strain 1037-ESALQ, registered commercially. According to the results both strains had similar virulence to adult T. limbativentris. commercially registered in Brazil market. According to the results, adults of T. Limbativentris were similarly susceptible to both strains of M. anisopliae. In the field experiment, five treatments were prepared: Fungus + Actara™ sublethal dose (50g ha), Fungus, Actara™ sublethal dose (50g./ha) Actara™ full dose (200g./ha) and control; which were applied on rice plants infested with insects inside nylon cages under field conditions. After seven and 14 days of application, 20 insects were collected from each cage and evaluated in the laboratory. According to the results, the evaluation of the first collection, mixture of M. anisopliae with 50g/ha Actara™ caused 54% mortality compared with 40, 32, 26 and 18% respectively for the full dose of Actara™ (200g/ha), fungus alone, Actara™ sublethal dose (50g/ha) and control. In the second collection, fungus + Actara™ highlightedin relation to the others, causing a 50% mortality, while treatment with the fungus alone the mortality was16% and the others treatment was 0% As a result, we found a synergism effect between fungus and sublethal dose of the insecticide. In summary, our results highlight the potential of sublethal concentrations of the neonicotinoid insecticide thiamethoxan in increasing the susceptibility of adults of T. Limbativentris to the fungus M. anisopliae. In fact, this strategy consists in a control method effective and feasible to combat this stink rice bug in the field from a viewpoint of an integrated pest management and sustainability.O presente estudo foi dividido em capítulos que inclui diversos aspectos da interação entre o fungo entomopatogênico M. anisopliae com o percevejo do colmo do arroz T. limbativentris como se seguem: O capítulo 1 (Considerações teóricas) descreve uma introdução sobre os elementos envolvidos neste estudo como: A importância da cultura do arroz para o Brasil, assim como aspectos sócio-econômicos e fitossanitário dessa cultura. O mesmo capítulo também aborda informações sobre o percevejo do colmo do arroz, Tibraca limbativentris stal (1869) como aspectos morfológicos, biológicos, importância como praga e métodos de controle químico e biológico. Sobre o fungo entomopatogênico Metarhizium anisopliae (Metsch.) Sorokin esse capítulo aborda aspectos gerais como taxonomia, morfologia, processo de infecção desse fungo aos seus hospedeiros e fatores de virulência. Para finalizar o capítulo foi explorado o assunto sobre ecologia química abordando as interações interespecíficas e intraespecíficas entre os insetos da família pentatomidae com ênfase nos estudos sobre compostos de defesas já elucidados nesta família de insetos. O capítulo 2 (Toxicidade de compostos extraídos da Glândulas Metatorácica (GMT), de adultos, e da Abdominal Dorsal (GAD), de ninfas, de T. limbativentris a M. anisopliae) descreve os resultados relacionados a diferença de susceptibilidade entre os estágios de vida do T. limbativentris ao fungo M. anisopliae e correlaciona estes resultados a atividade fungitóxica dos aldeídos (E)-2-octenal e (E)-2-decenal, extraído da glândula metatorácica (MTG) de adultos, e também o (E)-2-hexenal, extraído da glândula abdominal dorsal (DAG) nos estágios ninfais de segundo a quinto instar deste percevejo, por meio de coleta de exúvias. Todos os compostos extraídos foram caracterizados por CG-EM e o potencial fungitóxico determinado por meio da concentração inibitória mínima (MIC) desses compostos capaz de inibir totalmente a germinação do fungo. O aldeído (E)-2-decenal foi o mais ativo, apresentando MIC de 0,003% (30 ppm), em comparação com o (E)-2-hexenal e o (E)-2-octenal que apresentaram MIC de 0,012 e 0,025% respectivamente. O capítulo 3 (Efeito de agroquímicos convencionais utilizados na cultura do arroz ao fungo M. anisopliae.) descreve a avaliação da compatibilidade do M. anisopliae à oito inseticidas, cinco herbicidas e quatro fungicidas registrados para a cultura do arroz. Neste trabalho avaliou-se a germinação (GER), o crescimento vegetativo (CV) e a esporulação (ESP) do fungo após três horas em mistura, sob agitação, com os agroquímicos. Os parâmetros avaliados do fungo foram equacionados segundo a fórmula: IB = [(CV*47)+(ESP*43)+(GER*10)]/100; no qual através do valor índice biológico (IB) determinou-se a seletividade dos agroquímicos ao fungo. Entre os agroquímicos testados, os inseticidas Bravic™, Actara™, Karate Zeon™; os herbicidas Roundup™, Basagran™, Kifix™ e o fungicida Priori™ foram compatíveis ao M. anisopliae. No entanto, o fungicida Priori™ retardou a germinação do fungo, até 20 horas após o contato nenhum conídio germinado foi observado neste tratamento e após 48 horas foi observado 43% germinação. O capítulo 4 (Aumeto da patogenicidade de M. anisopliae a T. limbativentris com doses subletais de inseticidas químicos.) descreve a interação do M. anisopliae combinado com concentrações subletais de tiametoxam contra adultos do T. limbativentris. A combinação tem como hipótese superar resistência química natural do T. libentriventris ao M. anisopliae. Os resultados mostraram que o T. limbativentris foi mais susceptível ao M. anisopliae associado a doses subletais de tiametoxam (Actara™) ou λ-cialotrina (Karate Zeon™) do que ao xix fungo isolado. E ainda foi verificado uma concentração ótima para o fungo (5x107 conídios.mL), através de correlação com concentrações do inseticida tiametoxam. O fungo nesta concentração misturado ao tiametoxam a 3.1 ppm causou mortalidade de 80%, enquanto a taxa de mortalidade nos tratamentos com o fungo isolado e o tiametoxam 3.1 ppm foi de 38 e 35% respectivamente. As doses sobletais de Actara™ (3,1ppm) Karate Zeon™ (186ppm) foi estimada pela equação Probit. Com a intenção de utilizar uma cepa comercial do M. anisopliae foi comparada a virulência da cepa CG-168, utilizadas em todos os ensaios anteriores, com a cepa ESALQ-1037, registrada comercialmente. Segundo resultados ambas as cepas tiveram virulência semelhante para adultos de T. limbativentris. No experimento de campo, foram preparados cinco tratamentos: Fungo + Actara™ dose subletal (50g/há), Fungo, Actara™ dose subletal (50g/há), Actara™ dose cheia (200g/ha) e o controle; os quais foram aplicados nos insetos contidos em gaiolas em campo. Após um e sete dias da aplicação, foram coletados 20 insetos de cada gaiola e avaliados em laboratório. Segundo resultados, na avaliação da primeira coleta, o tratamento com a mistura do M. anisopliae com 50g/ha Actara™ apresentou taxa de mortalidade 54% contra 40, 32, 26 e 18% respectivamente para os tratamentos Actara™ dose cheia (200g/ha), Fungo, Actara™ dose subletal (50g/há) e Controle. Na avaliação da segunda coleta, o tratamento com o fungo + Actara™ destacou em relação aos demais, causando taxa de mortalidade de 50% enquanto o tratamento com o fungo isolado foi de 16% e os demais de 0%. Nesta avaliação constatou-se um sinergismo entre o fungo e a dose subletal do inseticida. Em resumo, nossos resultados destacam o potencial de concentrações subletais do inseticida tiametoxam (neonicotinóide) em aumentar a susceptibilidade dos adultos de T. limbativentris ao fungo M. anisopliae. Na verdade, esta estratégia consiste em um método de controle eficaz e viável para combater o percevejo do colmo do arroz em campo, do ponto de vista de um manejo integrado pragas com sustentabilidade.application/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/9899/Tese%20-%20Rodrigo%20Alves%20da%20Silva%20-%202012.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessControle biológicoFungo entomopatogênicoSustemtabilidadeBiological controlEntomophatogenic fungiSustainabilityQUIMICA::QUIMICA ORGANICAEstudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicosStudy of metarhizium anisopliae (metsch) sorok: toxicity of the compounds extracted from tibraca limbativentris stal (heteroptera: pentatomidae), effects of agrochemicals used in rice crops and enhanced pathogenicity to T. limbativentris with sublethal doses of chemical insecticidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6636939213254151586006006007826066743741197278-8194069717282802154reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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| spellingShingle | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos Silva , Rodrigo Alves da Controle biológico Fungo entomopatogênico Sustemtabilidade Biological control Entomophatogenic fungi Sustainability QUIMICA::QUIMICA ORGANICA |
| status_str | publishedVersion |
| title | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| title_full | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| title_fullStr | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| title_full_unstemmed | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| title_short | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| title_sort | Estudo de metarhizium anisopliae (metsch) sorok: toxicidade a compostos extraídos de tibraca limbativentris stal (heteroptera: pentatomidae), efeitos de agroquímicos utilizados na cultura do arroz e aumento da patogenicidade a T. limbativentris com doses subletais de inseticidas químicos |
| topic | Controle biológico Fungo entomopatogênico Sustemtabilidade Biological control Entomophatogenic fungi Sustainability QUIMICA::QUIMICA ORGANICA |
| url | http://repositorio.bc.ufg.br/tede/handle/tede/3310 |