Análise topográfica e histológica de enxertos de mucosa ceratinizada removidos com mucótomo ou bisturi convencional: estudo ex-vivo em mandíbula suína.
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| Autor principal: | |
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| Data de publicació: | 2022 |
| Format: | Master thesis |
| Idioma: | por |
| Font: | Repositório Institucional da UFG |
| Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/12463 |
Sumari: | To evaluate the surface and tissue quality of EMC obtained by the technique with conventional scalpel and mucotome. Methods: This is an experimental in vitro/ex vivo study. Six swine hemi-mandibles that had a favorable keratinized tissue condition were used for specimen removal in the anterior region of the mandible. Specimens were obtained using mucotome and conventional scalpel. For the purpose of greater precision, the procedure for removing specimens from the jaws was performed by a specialized and previously trained dental surgeon, offering the same conditions for graft removal by both techniques. random drawing to determine the technique to be performed for tissue removal. The specimens were prepared according to the protocols pre-established by the laboratories and then they were taken for analysis in optical microscopy to evaluate the epithelium and connective tissue and in scanning electron microscopy for analysis of topography and 3D profilometry. Results: Two areas were analyzed per sample with dimensions of 1920 x 2560 μm. It was possible to notice the presence of keratinized stratified squamous epithelial tissue and dense connective tissue modeled on the tissue slides removed with mucotome and scalpel. In the tissues removed with mucotome, there is a linearity in the tissue base and in the thickness of the tissue as a whole, presence of submucosa, fibrous connective tissue that is ideal for the success of the graft Differences in the surface of the samples removed by the two techniques were observed when to compare the images obtained through the two types of microscopy. Conclusion: Keratinized mucosa grafts when removed by the mucotome technique present greater uniformity and lower presence of undesirable cells compared to the scalpel technique, favoring a greater chance of success in surgical procedures for soft tissue grafting. |
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