Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção
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| Hlavní autor: | |
|---|---|
| Datum vydání: | 2019 |
| Médium: | Doctoral thesis |
| Jazyk: | por |
| Zdroj: | Repositório Institucional da UFG |
| Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/12205 |
Shrnutí: | Staphylococcus saprophyticus is a gram positive, coagulase negative bacteria and is one of the most common etiological agent of urinary tract infections (UTI) among sexually active young females. The infection may be led mainly by sexual intercourse. Little is known about the molecular features of S. saprophyticus infection model. However, plasticity in the proteomic profile is detected in the genus Staphylococcus and can be related with infection ability. In this study, we aimed to analyze the metabolic differences among three different pathogenic strains of S. saprophyticus (ATCC 15305, 7108 and 9325) using proteomics approaches. The proteomic data revealed variations among the strains that may lead to different responses in the context of an infection. There are differences in the carbohydrate metabolism, amino acid metabolism and pathogen defense against host defenses. Thioredoxin was one of the differentially expressed proteins among strains, thiol level assays showed similar levels of reduced thiol for ATCC15305 and 9325 strains, in contrast 7108 strain showed considerably low levels of reduced thiol levels when compared to the other analyzed strains. Urease is one of the main virulence factors of S. saprophyticus. The cytoplasmatic urease concentrations varied considerably. The 9325 strain had the lowest cytoplasmatic urease activity, however when plated on ureabased agar it showed a high urease activity, which means that probably most of the urease synthesized is exported to the extracellular milieu. Another important feature is related to the purine metabolism operon, which is highly regulated on ATCC15305 and exerts an influence upon the biofilm production in this strain. These results show that, although belonging to the same species, different S. saprophyticus strains present diverse behavior in response to different contexts that the microorganism may face in the host. |
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| author | Silva, Karla Christina Sousa |
| author_browse | Silva, Karla Christina Sousa |
| author_facet | Silva, Karla Christina Sousa |
| author_role | author |
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| collection | Repositório Institucional da UFG |
| contributor_str_mv | Rocha, Juliana Alves Parente Rocha, Juliana Alves Parente Marval, Márcia Giambiagi de Fontes, Wagner Kipnis, André Borges, Clayton Luiz |
| dc.contributor.advisor1.fl_str_mv | Rocha, Juliana Alves Parente |
| dc.contributor.advisor1Lattes.fl_str_mv | http://lattes.cnpq.br/7089231795367245 |
| dc.contributor.author.fl_str_mv | Silva, Karla Christina Sousa |
| dc.contributor.authorLattes.fl_str_mv | http://lattes.cnpq.br/5338622615751686 |
| dc.contributor.referee1.fl_str_mv | Rocha, Juliana Alves Parente |
| dc.contributor.referee2.fl_str_mv | Marval, Márcia Giambiagi de |
| dc.contributor.referee3.fl_str_mv | Fontes, Wagner |
| dc.contributor.referee4.fl_str_mv | Kipnis, André |
| dc.contributor.referee5.fl_str_mv | Borges, Clayton Luiz |
| dc.date.accessioned.fl_str_mv | 2022-07-22T11:38:37Z |
| dc.date.available.fl_str_mv | 2022-07-22T11:38:37Z |
| dc.date.issued.fl_str_mv | 2019-05-13 |
| dc.identifier.citation.fl_str_mv | SILVA, K. C. S. Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção. 2019. 101 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019. |
| dc.identifier.uri.fl_str_mv | http://repositorio.bc.ufg.br/tede/handle/tede/12205 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.country.fl_str_mv | Brasil |
| dc.publisher.department.fl_str_mv | Instituto de Ciências Biológicas - ICB (RG) |
| dc.publisher.initials.fl_str_mv | UFG |
| dc.publisher.none.fl_str_mv | Universidade Federal de Goiás |
| dc.publisher.program.fl_str_mv | Programa de Pós-graduação em Genética e Biologia Molecular (ICB) |
| dc.relation.cnpq.fl_str_mv | 109 |
| dc.relation.confidence.fl_str_mv | 500 500 500 500 |
| dc.relation.department.fl_str_mv | 23 |
| dc.relation.program.fl_str_mv | 58 |
| dc.relation.sponsorship.fl_str_mv | 1 |
| dc.rights.driver.fl_str_mv | Attribution-NonCommercial-NoDerivatives 4.0 International info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
| dc.subject.cnpq.fl_str_mv | CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
| dc.subject.eng.fl_str_mv | Staphylococcus saprophyticus Virulence Infection Proteomics |
| dc.subject.por.fl_str_mv | Proteômica Staphylococcus saprophyticus Virulência Infecção |
| dc.title.alternative.eng.fl_str_mv | Proteomic analysis of three strains of Staphylococcus saprophyticus elucidate different strategies for virulence and infection |
| dc.title.pt_BR.fl_str_mv | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/doctoralThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Staphylococcus saprophyticus is a gram positive, coagulase negative bacteria and is one of the most common etiological agent of urinary tract infections (UTI) among sexually active young females. The infection may be led mainly by sexual intercourse. Little is known about the molecular features of S. saprophyticus infection model. However, plasticity in the proteomic profile is detected in the genus Staphylococcus and can be related with infection ability. In this study, we aimed to analyze the metabolic differences among three different pathogenic strains of S. saprophyticus (ATCC 15305, 7108 and 9325) using proteomics approaches. The proteomic data revealed variations among the strains that may lead to different responses in the context of an infection. There are differences in the carbohydrate metabolism, amino acid metabolism and pathogen defense against host defenses. Thioredoxin was one of the differentially expressed proteins among strains, thiol level assays showed similar levels of reduced thiol for ATCC15305 and 9325 strains, in contrast 7108 strain showed considerably low levels of reduced thiol levels when compared to the other analyzed strains. Urease is one of the main virulence factors of S. saprophyticus. The cytoplasmatic urease concentrations varied considerably. The 9325 strain had the lowest cytoplasmatic urease activity, however when plated on ureabased agar it showed a high urease activity, which means that probably most of the urease synthesized is exported to the extracellular milieu. Another important feature is related to the purine metabolism operon, which is highly regulated on ATCC15305 and exerts an influence upon the biofilm production in this strain. These results show that, although belonging to the same species, different S. saprophyticus strains present diverse behavior in response to different contexts that the microorganism may face in the host. |
| eu_rights_str_mv | openAccess |
| format | doctoralThesis |
| id | UFG-2_b0c7a04c0abe87c8a04fdc818efeb5ac |
| identifier_str_mv | SILVA, K. C. S. Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção. 2019. 101 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019. |
| instacron_str | UFG |
| institution | UFG |
| instname_str | Universidade Federal de Goiás (UFG) |
| language | por |
| network_acronym_str | UFG-2 |
| network_name_str | Repositório Institucional da UFG |
| oai_identifier_str | oai:repositorio.bc.ufg.br:tede/12205 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Universidade Federal de Goiás |
| reponame_str | Repositório Institucional da UFG |
| repository.mail.fl_str_mv | grt.bc@ufg.br |
| repository.name.fl_str_mv | Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
| repository_id_str | oai:repositorio.bc.ufg.br:tede/1234 |
| rights_invalid_str_mv | Attribution-NonCommercial-NoDerivatives 4.0 International |
| spelling | Rocha, Juliana Alves Parentehttp://lattes.cnpq.br/7089231795367245Rocha, Juliana Alves ParenteMarval, Márcia Giambiagi deFontes, WagnerKipnis, AndréBorges, Clayton Luizhttp://lattes.cnpq.br/5338622615751686Silva, Karla Christina Sousa2022-07-22T11:38:37Z2022-07-22T11:38:37Z2019-05-13SILVA, K. C. S. Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção. 2019. 101 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/12205Staphylococcus saprophyticus is a gram positive, coagulase negative bacteria and is one of the most common etiological agent of urinary tract infections (UTI) among sexually active young females. The infection may be led mainly by sexual intercourse. Little is known about the molecular features of S. saprophyticus infection model. However, plasticity in the proteomic profile is detected in the genus Staphylococcus and can be related with infection ability. In this study, we aimed to analyze the metabolic differences among three different pathogenic strains of S. saprophyticus (ATCC 15305, 7108 and 9325) using proteomics approaches. The proteomic data revealed variations among the strains that may lead to different responses in the context of an infection. There are differences in the carbohydrate metabolism, amino acid metabolism and pathogen defense against host defenses. Thioredoxin was one of the differentially expressed proteins among strains, thiol level assays showed similar levels of reduced thiol for ATCC15305 and 9325 strains, in contrast 7108 strain showed considerably low levels of reduced thiol levels when compared to the other analyzed strains. Urease is one of the main virulence factors of S. saprophyticus. The cytoplasmatic urease concentrations varied considerably. The 9325 strain had the lowest cytoplasmatic urease activity, however when plated on ureabased agar it showed a high urease activity, which means that probably most of the urease synthesized is exported to the extracellular milieu. Another important feature is related to the purine metabolism operon, which is highly regulated on ATCC15305 and exerts an influence upon the biofilm production in this strain. These results show that, although belonging to the same species, different S. saprophyticus strains present diverse behavior in response to different contexts that the microorganism may face in the host.Staphylococcus saprophyticus é uma bactéria Gram-positiva, coagulase negativa e é um dos agentes etiológicos mais comuns de infecções do trato urinário (ITU) em mulheres jovens sexualmente ativas. A infecção pode ser adquirida principalmente via relação sexual. Pouco se sabe sobre suas características moleculares no modelo de S. saprophyticus. No entanto, a plasticidade no perfil proteômico é detectada no gênero Staphylococcus e pode estar relacionada com a capacidade de infecção. Neste estudo, objetivou-se analisar as diferenças metabólicas entre três diferentes cepas patogênicasde S. saprophyticus (ATCC 15305, 7108 e 9325) utilizando abordagens proteômicas. Os dados proteômicos revelaram variações entre as cepas que podem levar a diferentes respostas no contexto de uma infecção. Existem diferenças no metabolismo de carboidratos, no metabolismo de aminoácidos e na defesa de patógenos contra as defesas do hospedeiro. A tiorredoxina foi uma das proteínas diferencialmente expressas entre as cepas; os níveis de tiol mostraram níveis semelhantes de tiol reduzido para as cepas ATCC15305 e 9325; em contraste, a cepa 7108 mostrou níveis consideravelmente baixos de níveis tiol reduzidos quando comparados com as outras cepas analisadas. A urease é um dos principais fatores de virulência do S. saprophyticus. As concentrações de urease citoplasmática variaram consideravelmente. A linhagem 9325 apresentou a menor atividade da urease citoplasmática, porém quando aplicada em ágar à base de uréia, apresenta alta atividade de urease, o que significa que provavelmente a maior parte da urease sintetizada é exportada para o meio extracelular. As cepas também foram analisadas. Outra característica importante está relacionada ao operon do metabolismo das purinas, que é altamente regulado na ATCC15305 e exerce uma influência sobre a produção de biofilme nesta linhagem. Estes resultados mostram que, apesar de pertencerem à mesma espécie, diferentes linhagens de S. saprophyticus apresentam comportamento diverso em resposta a diferentes contextos que o microrganismo pode enfrentar no hospedeiro.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccessProteômicaStaphylococcus saprophyticusVirulênciaInfecçãoStaphylococcus saprophyticusVirulenceInfectionProteomicsCIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIAAnálises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecçãoProteomic analysis of three strains of Staphylococcus saprophyticus elucidate different strategies for virulence and infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis58500500500500231091reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/4b0e8939-7edc-4553-ae07-4b08a1b8f9cf/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/6596ae0e-04c7-44d3-bcf8-22e3ab9223c9/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTese - Karla Christina Sousa Silva - 2019.pdfTese - Karla Christina Sousa Silva - 2019.pdfapplication/pdf2047250http://repositorio.bc.ufg.br/tede/bitstreams/abf1032f-9de1-4a17-8c7b-becd36caad13/download23da03f70b50c11e76a33943baaae757MD53tede/122052022-07-22 08:38:37.359http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12205http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttps://repositorio.bc.ufg.br/tedeserver/oai/requestgrt.bc@ufg.bropendoar:oai:repositorio.bc.ufg.br:tede/12342022-07-22T11:38:37Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
| spellingShingle | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção Silva, Karla Christina Sousa Proteômica Staphylococcus saprophyticus Virulência Infecção Staphylococcus saprophyticus Virulence Infection Proteomics CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
| status_str | publishedVersion |
| title | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| title_full | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| title_fullStr | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| title_full_unstemmed | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| title_short | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| title_sort | Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção |
| topic | Proteômica Staphylococcus saprophyticus Virulência Infecção Staphylococcus saprophyticus Virulence Infection Proteomics CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
| url | http://repositorio.bc.ufg.br/tede/handle/tede/12205 |