Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus)
Salvato in:
| Autore principale: | |
|---|---|
| Data di pubblicazione: | 2017 |
| Natura: | Master thesis |
| Lingua: | por |
| Fonte: | Repositório Institucional da UFG |
| Download full: | http://repositorio.bc.ufg.br/tede/handle/tede/7052 |
Riassunto: | This study aimed to evaluate the viability of the decellularization process in bovine cartilaginous tissue by histological analysis. For this purpose, 20 bovine elastic cartilages were used, immersed in alkaline solution for 72 hours, followed by washing, freezing and lyophilization of the material. Histological processing was then performed to perform the analyzes. In the histological analyzes the decellularization and removal of the amorphous substance from the tissue was observed, but the structure of elastic fibers and collagen remained. It was concluded that the alkaline treatment was efficient to obtain biomaterial from elastic cartilaginous tissue. This study also evaluated, through thermographic examination, the feasibility of using implants with biomaterials obtained from elastic cartilages treated in alkaline solution. The intensity of the inflammatory process was observed through the variation of cutaneous temperature comparing before and after the implantation of the biomaterials in rabbits (Oryctolagus cuniculus). A total of 24 rabbits were evaluated, divided into two groups, of 12 animals each, according to the implanted biomaterial. Evaluations were performed in the preoperative period and after the implantation of biomaterials. In the (GI) group, polypropylene mesh and elastic cartilage (GII) were treated with alkaline solution, evaluated in the periods of seven and 35 days. From the results obtained, after performing statistical tests, the group (GI) polypropylene mesh, obtained upper abdominal skin temperatures, compared to the cartilage group, providing subsidies that the use of elastic cartilage treated in alkaline solution, promoted lower inflammatory intensity due to the processing to which it was submitted. It is concluded that treated elastic cartilage has been shown to be a superior biomaterial relative to the mesh polypropylene. |
| _version_ | 1871426328981405696 |
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| author | Ferreira, Kamilla Dias |
| author_browse | Ferreira, Kamilla Dias |
| author_facet | Ferreira, Kamilla Dias |
| author_role | author |
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| collection | Repositório Institucional da UFG |
| contributor_str_mv | Vulcani, Valcinir Aloisio Scalla Silva, Luiz Antônio Franco da Vulcani, Valcinir Aloisio Scalla Damasceno, Adilson Donizeti Martins, Apóstolo Ferreira |
| dc.contributor.advisor-co1.fl_str_mv | Silva, Luiz Antônio Franco da |
| dc.contributor.advisor-co1Lattes.fl_str_mv | http://lattes.cnpq.br/0446055905975647 |
| dc.contributor.advisor1.fl_str_mv | Vulcani, Valcinir Aloisio Scalla |
| dc.contributor.advisor1Lattes.fl_str_mv | http://lattes.cnpq.br/9821938265591545 |
| dc.contributor.author.fl_str_mv | Ferreira, Kamilla Dias |
| dc.contributor.authorLattes.fl_str_mv | http://lattes.cnpq.br/8844076703503119 |
| dc.contributor.referee1.fl_str_mv | Vulcani, Valcinir Aloisio Scalla |
| dc.contributor.referee2.fl_str_mv | Damasceno, Adilson Donizeti |
| dc.contributor.referee3.fl_str_mv | Martins, Apóstolo Ferreira |
| dc.date.accessioned.fl_str_mv | 2017-04-03T11:40:34Z |
| dc.date.issued.fl_str_mv | 2017-02-06 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.citation.fl_str_mv | FERREIRA, K. D. Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus). 2017. 68 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017. |
| dc.identifier.uri.fl_str_mv | http://repositorio.bc.ufg.br/tede/handle/tede/7052 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.country.fl_str_mv | Brasil |
| dc.publisher.department.fl_str_mv | Escola de Veterinária e Zootecnia - EVZ (RG) |
| dc.publisher.initials.fl_str_mv | UFG |
| dc.publisher.none.fl_str_mv | Universidade Federal de Goiás |
| dc.publisher.program.fl_str_mv | Programa de Pós-graduação em Ciência Animal (EVZ) |
| dc.relation.cnpq.fl_str_mv | 453670264235017319 |
| dc.relation.confidence.fl_str_mv | 600 600 600 600 |
| dc.relation.department.fl_str_mv | -6217552114249094582 |
| dc.relation.program.fl_str_mv | 4581960685150189167 |
| dc.relation.sponsorship.fl_str_mv | 2075167498588264571 |
| dc.rights.driver.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
| dc.subject.cnpq.fl_str_mv | CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| dc.subject.eng.fl_str_mv | Biomaterials Elastic cartilage Surgery Rabbits Alkaline treatment Thermography |
| dc.subject.por.fl_str_mv | Biomateriais Cartilagem elástica Cirurgia Coelhos Tratamento alcalino Termografia |
| dc.title.por.fl_str_mv | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | This study aimed to evaluate the viability of the decellularization process in bovine cartilaginous tissue by histological analysis. For this purpose, 20 bovine elastic cartilages were used, immersed in alkaline solution for 72 hours, followed by washing, freezing and lyophilization of the material. Histological processing was then performed to perform the analyzes. In the histological analyzes the decellularization and removal of the amorphous substance from the tissue was observed, but the structure of elastic fibers and collagen remained. It was concluded that the alkaline treatment was efficient to obtain biomaterial from elastic cartilaginous tissue. This study also evaluated, through thermographic examination, the feasibility of using implants with biomaterials obtained from elastic cartilages treated in alkaline solution. The intensity of the inflammatory process was observed through the variation of cutaneous temperature comparing before and after the implantation of the biomaterials in rabbits (Oryctolagus cuniculus). A total of 24 rabbits were evaluated, divided into two groups, of 12 animals each, according to the implanted biomaterial. Evaluations were performed in the preoperative period and after the implantation of biomaterials. In the (GI) group, polypropylene mesh and elastic cartilage (GII) were treated with alkaline solution, evaluated in the periods of seven and 35 days. From the results obtained, after performing statistical tests, the group (GI) polypropylene mesh, obtained upper abdominal skin temperatures, compared to the cartilage group, providing subsidies that the use of elastic cartilage treated in alkaline solution, promoted lower inflammatory intensity due to the processing to which it was submitted. It is concluded that treated elastic cartilage has been shown to be a superior biomaterial relative to the mesh polypropylene. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | UFG-2_1b01d54dd295bc84d0c8376f86b3fa2e |
| identifier_str_mv | FERREIRA, K. D. Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus). 2017. 68 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017. |
| instacron_str | UFG |
| institution | UFG |
| instname_str | Universidade Federal de Goiás (UFG) |
| language | por |
| network_acronym_str | UFG-2 |
| network_name_str | Repositório Institucional da UFG |
| oai_identifier_str | oai:repositorio.bc.ufg.br:tede/7052 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher.none.fl_str_mv | Universidade Federal de Goiás |
| reponame_str | Repositório Institucional da UFG |
| repository.mail.fl_str_mv | grt.bc@ufg.br |
| repository.name.fl_str_mv | Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
| repository_id_str | oai:repositorio.bc.ufg.br:tede/1234 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Vulcani, Valcinir Aloisio Scallahttp://lattes.cnpq.br/9821938265591545Silva, Luiz Antônio Franco dahttp://lattes.cnpq.br/0446055905975647Vulcani, Valcinir Aloisio ScallaDamasceno, Adilson DonizetiMartins, Apóstolo Ferreirahttp://lattes.cnpq.br/8844076703503119Ferreira, Kamilla Dias2017-04-03T11:40:34Z2017-02-06FERREIRA, K. D. Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus). 2017. 68 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/7052This study aimed to evaluate the viability of the decellularization process in bovine cartilaginous tissue by histological analysis. For this purpose, 20 bovine elastic cartilages were used, immersed in alkaline solution for 72 hours, followed by washing, freezing and lyophilization of the material. Histological processing was then performed to perform the analyzes. In the histological analyzes the decellularization and removal of the amorphous substance from the tissue was observed, but the structure of elastic fibers and collagen remained. It was concluded that the alkaline treatment was efficient to obtain biomaterial from elastic cartilaginous tissue. This study also evaluated, through thermographic examination, the feasibility of using implants with biomaterials obtained from elastic cartilages treated in alkaline solution. The intensity of the inflammatory process was observed through the variation of cutaneous temperature comparing before and after the implantation of the biomaterials in rabbits (Oryctolagus cuniculus). A total of 24 rabbits were evaluated, divided into two groups, of 12 animals each, according to the implanted biomaterial. Evaluations were performed in the preoperative period and after the implantation of biomaterials. In the (GI) group, polypropylene mesh and elastic cartilage (GII) were treated with alkaline solution, evaluated in the periods of seven and 35 days. From the results obtained, after performing statistical tests, the group (GI) polypropylene mesh, obtained upper abdominal skin temperatures, compared to the cartilage group, providing subsidies that the use of elastic cartilage treated in alkaline solution, promoted lower inflammatory intensity due to the processing to which it was submitted. It is concluded that treated elastic cartilage has been shown to be a superior biomaterial relative to the mesh polypropylene.Este estudo objetivou avaliar a viabilidade do processamento de descelularização em tecido cartilaginoso bovino por meio de análises histológicas. Pra tanto, foram utilizadas 20 cartilagens elásticas de bovinos, submetidas à imersão em solução alcalina por 72 horas, seguidos de lavagens, congelamento e liofilização do material. Em seguida foi realizado o processamento histológico para serem feitas as analises. Nas analises histológicas foi observado a descelularização e retirada da substancia amorfa do tecido, porém manteve a estrutura das fibras elásticas e de colágeno. Concluiu-se que o tratamento alcalino foi eficiente para obtenção de biomaterial a partir de tecido cartilaginoso elástico. Este estudo avaliou também, através do exame termográfico, a viabilidade no uso de implantes com biomateriais obtidos de cartilagens elásticas tratadas em solução alcalina. Foram observados através da variação da temperatura cutânea, a intensidade do processo inflamatório comparando antes e após a implantação dos biomateriais em coelhos (Oryctolagus cuniculus). Para tanto foram avaliados um total de 24 coelhos, divididos em dois grupos, de 12 animais, de acordo com o biomaterial implantado. Foram realizadas avaliações no período pré-operatório e após o implante dos biomateriais. No grupo (GI) foi implantada a tela de polipropileno e (GII) a cartilagem elástica tratada em solução alcalina, avaliados nos períodos de sete e 35 dias. Dos resultados obtidos, após realização de testes estatísticos, o grupo (GI), obteve temperaturas cutâneas abdominais superiores, comparadas ao grupo cartilagem, fornecendo, subsídios de que a utilização da cartilagem elástica tratada em solução alcalina, promoveu menor intensidade inflamatória devido ao processamento ao qual foi submetida. Conclui-se que a cartilagem elástica tratada demonstrou ser um biomaterial superior em relação à tela de polipropileno.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBiomateriaisCartilagem elásticaCirurgiaCoelhosTratamento alcalinoTermografiaBiomaterialsElastic cartilageSurgeryRabbitsAlkaline treatmentThermographyCIENCIAS AGRARIAS::MEDICINA VETERINARIACartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis4581960685150189167600600600600-62175521142490945824536702642350173192075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGCC-LICENSElicense_urllicense_urltext/plain; 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| spellingShingle | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) Ferreira, Kamilla Dias Biomateriais Cartilagem elástica Cirurgia Coelhos Tratamento alcalino Termografia Biomaterials Elastic cartilage Surgery Rabbits Alkaline treatment Thermography CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| status_str | publishedVersion |
| title | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| title_full | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| title_fullStr | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| title_full_unstemmed | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| title_short | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| title_sort | Cartilagem auricular de bovinos tratada com solução alcalina: avaliação histológica pré- implantação e termográfica pós-implantação na parede abdominal de coelhos (Oryctolagus cuniculus) |
| topic | Biomateriais Cartilagem elástica Cirurgia Coelhos Tratamento alcalino Termografia Biomaterials Elastic cartilage Surgery Rabbits Alkaline treatment Thermography CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| url | http://repositorio.bc.ufg.br/tede/handle/tede/7052 |