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Microbioma do leite de bovinos das raças Curraleiro Pé-Duro e Pantaneiro

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Hlavní autor: Soares, Nayana Ribeiro
Datum vydání: 2019
Médium: Doctoral thesis
Jazyk: por
Zdroj: Repositório Institucional da UFG
Download full: http://repositorio.bc.ufg.br/tede/handle/tede/10102
Shrnutí: The objective of the present study was to describe the microbiota of raw milk from naturalized bovine breeds, such as Curraleiro Pé- Duro and Pantaneiro and to increase the value of these herds, through their use as specific genetic banks of microorganisms. Attention is also given to the possible technological application of microbial populations present in the udder and milk of these breeds. Six samples of milk from the Curraleiro Pé-Duro herd were collected at the State University of Mato Grosso do Sul, University Unit of Aquidauana, while 15 samples from the Pantaneiro herd were collected from the Aquidauana Center for Pantaneiro Cattle Conservation also belonging to the State University of Mato Grosso do Sul, University Unit of Aquidauana .From the results found in this study, the Somatic Cell Count and the centesimal composition were determined to identify lactic acid bacteria present in the raw milk and to recognize those isolated lactic acid bacteria both by phenotype and genotype that produce bacteriocins with inhibitory activity against food pathogens and their susceptibility to antimicrobials. In addition, the genes present and protein sequences were described. The results suggest that, even though it is a herd of low milk production, it presents quality. The microbiota that makes up the Curraleiro Pé-Duro and Pantaneiro breeds is specifically interesting with regard to the functional use of lactic acid bacteria. Enterococcus faecium was the dominant species, with phenotypic variability for the production of bacteriocins and resistance to vancomycin and penicillin. Pantaneiro cattle isolates were known to produce bacteriocins anti- Listeria monocytogenes and with genome elements that show double suitability for agroindustrial use, as well as risk to animal and human health.
Shrnutí:The objective of the present study was to describe the microbiota of raw milk from naturalized bovine breeds, such as Curraleiro Pé- Duro and Pantaneiro and to increase the value of these herds, through their use as specific genetic banks of microorganisms. Attention is also given to the possible technological application of microbial populations present in the udder and milk of these breeds. Six samples of milk from the Curraleiro Pé-Duro herd were collected at the State University of Mato Grosso do Sul, University Unit of Aquidauana, while 15 samples from the Pantaneiro herd were collected from the Aquidauana Center for Pantaneiro Cattle Conservation also belonging to the State University of Mato Grosso do Sul, University Unit of Aquidauana .From the results found in this study, the Somatic Cell Count and the centesimal composition were determined to identify lactic acid bacteria present in the raw milk and to recognize those isolated lactic acid bacteria both by phenotype and genotype that produce bacteriocins with inhibitory activity against food pathogens and their susceptibility to antimicrobials. In addition, the genes present and protein sequences were described. The results suggest that, even though it is a herd of low milk production, it presents quality. The microbiota that makes up the Curraleiro Pé-Duro and Pantaneiro breeds is specifically interesting with regard to the functional use of lactic acid bacteria. Enterococcus faecium was the dominant species, with phenotypic variability for the production of bacteriocins and resistance to vancomycin and penicillin. Pantaneiro cattle isolates were known to produce bacteriocins anti- Listeria monocytogenes and with genome elements that show double suitability for agroindustrial use, as well as risk to animal and human health.

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