Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí
Guardat en:
| Autor principal: | |
|---|---|
| Data de publicació: | 2023 |
| Format: | Master thesis |
| Idioma: | por |
| Font: | Repositório Comum do Brasil - Deposita |
| Download full: | https://deposita.ibict.br/handle/deposita/516 |
Sumari: | Tucuruí is the second largest Brazilian Hydroelectric Power Plant, the artificial lake in the municipality of Tucuruí has altered the ecosystem of the region. Many free-living microorganisms responsible for biomass degradation, organic matter decomposition, biogeochemical cycles, some of clinical interest, are adapted to live in the lake reservoir. This research aims to obtain bacterial genomes by metagenomic data and identify functional profiles and microbial metabolisms in the lake. Water and sediment samples were collected in the lake of the Tucuruí Hydroelectric Power Plant in the photic, aphotic and sedimentary layers of the station upstream of the new division. After filtering through a nitrocellulose membrane, the DNA samples were extracted. Total metagenome sequencing was performed on the Ion ProtonTM platform. Megahit was used to assemble the metagenomes. ORFs were predicted using Prodigal and BUSCO was used for the quantification of completeness metrics for comparative evaluation of single-copy universal orthologues. Genomes assembled into metagenomes were classified in the databases. The phyla were defined at the genus and species level, where a diverse and extremely conserved microbiota was found. The functional profile of the metagenomes found corroborated with other studies of metagenomes of oligotrophic lakes, demonstrating high biochemical, conserved and diverse activity, being a pioneering study when assembling the first metagenomes of samples from the Tucuruí Hydroelectric Power Plant. |
| _version_ | 1872168167336312832 |
|---|---|
| author | Padilha, Marcos Daniel Mendes |
| author_browse | Padilha, Marcos Daniel Mendes |
| author_facet | Padilha, Marcos Daniel Mendes |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 MD5 |
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| collection | Repositório Comum do Brasil - Deposita |
| contributor_str_mv | Graças, Diego Assis das |
| dc.contributor.advisor1.fl_str_mv | Graças, Diego Assis das |
| dc.contributor.author.fl_str_mv | Padilha, Marcos Daniel Mendes |
| dc.contributor.authorLattes.fl_str_mv | http://lattes.cnpq.br/7402939637915735 |
| dc.date.accessioned.fl_str_mv | 2024-02-18T22:58:09Z |
| dc.date.issued.fl_str_mv | 2023 |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | https://deposita.ibict.br/handle/deposita/516 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.country.fl_str_mv | Brasil |
| dc.publisher.department.fl_str_mv | Universidade Federal do Pará, Instituto de Ciências Biológicas |
| dc.publisher.none.fl_str_mv | Universidade Federal do Pará |
| dc.publisher.program.fl_str_mv | Programa de Pós-graduação em Genética e Biologia Molecular |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Comum do Brasil - Deposita instname:Instituto Brasileiro de Informação em Ciência e Tecnologia (Ibict) instacron:IBICT |
| dc.subject.cnpq.fl_str_mv | Ciências Biológicas Genética de microrganismos |
| dc.subject.por.fl_str_mv | Adaptação microbiana Metagenoma de lago Bactérias de lagos de água doce Bioquímica e metabolismo microbiano |
| dc.title.por.fl_str_mv | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Tucuruí is the second largest Brazilian Hydroelectric Power Plant, the artificial lake in the municipality of Tucuruí has altered the ecosystem of the region. Many free-living microorganisms responsible for biomass degradation, organic matter decomposition, biogeochemical cycles, some of clinical interest, are adapted to live in the lake reservoir. This research aims to obtain bacterial genomes by metagenomic data and identify functional profiles and microbial metabolisms in the lake. Water and sediment samples were collected in the lake of the Tucuruí Hydroelectric Power Plant in the photic, aphotic and sedimentary layers of the station upstream of the new division. After filtering through a nitrocellulose membrane, the DNA samples were extracted. Total metagenome sequencing was performed on the Ion ProtonTM platform. Megahit was used to assemble the metagenomes. ORFs were predicted using Prodigal and BUSCO was used for the quantification of completeness metrics for comparative evaluation of single-copy universal orthologues. Genomes assembled into metagenomes were classified in the databases. The phyla were defined at the genus and species level, where a diverse and extremely conserved microbiota was found. The functional profile of the metagenomes found corroborated with other studies of metagenomes of oligotrophic lakes, demonstrating high biochemical, conserved and diverse activity, being a pioneering study when assembling the first metagenomes of samples from the Tucuruí Hydroelectric Power Plant. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | IBICT-1_c52e06bd8080dbbf03d9d85b46412d90 |
| instacron_str | IBICT |
| institution | IBICT |
| instname_str | Instituto Brasileiro de Informação em Ciência e Tecnologia (Ibict) |
| language | por |
| network_acronym_str | IBICT-1 |
| network_name_str | Repositório Comum do Brasil - Deposita |
| oai_identifier_str | oai:deposita.ibict.br:deposita/516 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | Universidade Federal do Pará |
| reponame_str | Repositório Comum do Brasil - Deposita |
| repository.mail.fl_str_mv | deposita@ibict.br |
| repository.name.fl_str_mv | Repositório Comum do Brasil - Deposita - Instituto Brasileiro de Informação em Ciência e Tecnologia (Ibict) |
| repository_id_str | 4658 |
| spelling | Graças, Diego Assis dashttp://lattes.cnpq.br/7402939637915735Padilha, Marcos Daniel Mendes2024-02-18T22:58:09Z2023https://deposita.ibict.br/handle/deposita/516Tucuruí is the second largest Brazilian Hydroelectric Power Plant, the artificial lake in the municipality of Tucuruí has altered the ecosystem of the region. Many free-living microorganisms responsible for biomass degradation, organic matter decomposition, biogeochemical cycles, some of clinical interest, are adapted to live in the lake reservoir. This research aims to obtain bacterial genomes by metagenomic data and identify functional profiles and microbial metabolisms in the lake. Water and sediment samples were collected in the lake of the Tucuruí Hydroelectric Power Plant in the photic, aphotic and sedimentary layers of the station upstream of the new division. After filtering through a nitrocellulose membrane, the DNA samples were extracted. Total metagenome sequencing was performed on the Ion ProtonTM platform. Megahit was used to assemble the metagenomes. ORFs were predicted using Prodigal and BUSCO was used for the quantification of completeness metrics for comparative evaluation of single-copy universal orthologues. Genomes assembled into metagenomes were classified in the databases. The phyla were defined at the genus and species level, where a diverse and extremely conserved microbiota was found. The functional profile of the metagenomes found corroborated with other studies of metagenomes of oligotrophic lakes, demonstrating high biochemical, conserved and diverse activity, being a pioneering study when assembling the first metagenomes of samples from the Tucuruí Hydroelectric Power Plant.Tucuruí é a segunda maior Usina Hidrelétrica brasileira, o lago artificial no município de Tucuruí alterou o ecossistema da região. Muitos microrganismos de vida livre responsáveis pela degradação de biomassa, decomposição de matéria orgânica, ciclos biogeoquímicos, alguns de interesse clínico, estão adaptados para viver no reservatório do lago. Esta pesquisa tem por objetivo a obtenção de genomas bacterianos por dados metagenômicos e identificar perfis funcionais e metabolismos microbianos no lago. Amostras de água e sedimento foram coletadas no lago da Usina Hidrelétrica de Tucuruí nas camadas fótica, afótica e sedimentar da estação do montante do novo repartimento. Após a filtração em membrana de nitrocelulose as amostras de DNA foram extraídas. O sequenciamento de metagenoma total foi realizado na plataforma Ion ProtonTM. Utilizou-se o Megahit para a montagem dos metagenomas. ORFs foram previstas usando Prodigal e foi usado o BUSCO para as quantificações de métricas de completude para avaliação comparativa de ortólogos universais de cópia única. Os genomas montados em metagenomas foram classificados nos banco de dados. Os filos foram definidos em nível de gênero e espécie, onde foi encontrado uma microbiota diversa e extremamente conservada. O perfil funcional dos metagenomas encontrados corroborou com outros estudos de metagenomas de lagos oligotróficos, demonstrando alta atividade bioquímica, conservada e diversa, sendo um estudo pioneiro ao montar os primeiros metagenomas de amostras da Usina Hidrelétrica de Tucuruí.CNPqNorte-1application/pdfapplication/pdfporUniversidade Federal do ParáPrograma de Pós-graduação em Genética e Biologia MolecularBrasilUniversidade Federal do Pará, Instituto de Ciências BiológicasAdaptação microbianaMetagenoma de lagoBactérias de lagos de água doceBioquímica e metabolismo microbianoCiências BiológicasGenética de microrganismosObtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruíinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Comum do Brasil - Depositainstname:Instituto Brasileiro de Informação em Ciência e Tecnologia (Ibict)instacron:IBICTTEXTDISSERTAÇÃO DE MESTRADO EM GENÉTICA E BIOLOGIA MOLECULAR-mesclado.pdf.txtWritten by FormatFilter org.dspace.app.mediafilter.TikaTextExtractionFilter on 2025-06-06T20:14:56Z (GMT).Extracted texttext/plain105311https://deposita.ibict.br/bitstreams/0cd88353-b2c4-43be-bec0-268fb85dfac1/download14806b9856d952d84a69545d305dbe7aMD53falseAnonymousREADTHUMBNAILDISSERTAÇÃO DE MESTRADO EM GENÉTICA E BIOLOGIA MOLECULAR-mesclado.pdf.jpgWritten by FormatFilter org.dspace.app.mediafilter.PDFBoxThumbnail on 2025-06-06T20:14:57Z (GMT).Generated Thumbnailimage/jpeg2648https://deposita.ibict.br/bitstreams/804566e5-c3e7-44a6-b0cd-e632afdf2377/download62c449edef67995c8dc4be934b02817dMD54falseAnonymousREADLICENSElicense.txtWritten by org.dspace.content.LicenseUtilstext/plain; 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| spellingShingle | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí Padilha, Marcos Daniel Mendes Adaptação microbiana Metagenoma de lago Bactérias de lagos de água doce Bioquímica e metabolismo microbiano Ciências Biológicas Genética de microrganismos |
| status_str | publishedVersion |
| title | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| title_full | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| title_fullStr | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| title_full_unstemmed | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| title_short | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| title_sort | Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí |
| topic | Adaptação microbiana Metagenoma de lago Bactérias de lagos de água doce Bioquímica e metabolismo microbiano Ciências Biológicas Genética de microrganismos |
| url | https://deposita.ibict.br/handle/deposita/516 |