Estabilidade e potencial antioxidante de microcápsulas de ficocianina

Bibliographic Details
Main Author: Formigheri, Mariana da Silva
Publication Date: 2017
Format: Master thesis
Language: por
Source: Repositório Institucional da UPF
Download full: https://repositorio.upf.br/handle/123456789/1692
Summary: Interest in bioactive compounds research has increased as consumers look for natural products that result in health benefits. The stability of these compounds, in the great majority, however is of unknown behavior. The use of techniques to ensure the functionality of these assets for different purposes becomes very important. Encapsulation is a technology capable of protecting the active substance from a wide range of exposure conditions, promoting controlled release, masking unpleasant odor and taste, and promoting the homogenous dilution of the encapsulated material in food products or pharmaceutical formulations. Phycocyanin is a phycobiliprotein, present in the cyanobacterium Spirulina platensis, and has been reported to have different functional properties, among them, the antioxidant. The objective of this study was to study the microencapsulation by spray-drying of phycocyanin, using the encapsulant maltodextrin, in stability tests and antioxidant potential in vitro and in vivo, in Saccharomyces cerevisiae cells. The results show that the encapsulation process produced spherical microcapsules of average size of 10 µm, with water solubility around 91.71%, water and moisture activity of 0.209 and 3.975%, respectively. Maltodextrin protected the active's physical agent temperature and promoted gradual release of the antioxidant potential and maintained the same after drying. In the in vivo tests the microcapsules statistically increased the enzymatic activity of glutathione and decreased levels of lipid peroxidation. It was concluded thatthe technique of encapsulating phycocyanin by spray dryer with the encapsulant maltodextrin was effective in maintaining the antioxidant activity of bioactive.
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spelling Estabilidade e potencial antioxidante de microcápsulas de ficocianinaStability and antioxidant potential of phycocyanin microcapsulesEncapsulaçãoFuncionalidadeSpirulina platensisCIENCIA E TECNOLOGIA DE ALIMENTOS::ENGENHARIA DE ALIMENTOSInterest in bioactive compounds research has increased as consumers look for natural products that result in health benefits. The stability of these compounds, in the great majority, however is of unknown behavior. The use of techniques to ensure the functionality of these assets for different purposes becomes very important. Encapsulation is a technology capable of protecting the active substance from a wide range of exposure conditions, promoting controlled release, masking unpleasant odor and taste, and promoting the homogenous dilution of the encapsulated material in food products or pharmaceutical formulations. Phycocyanin is a phycobiliprotein, present in the cyanobacterium Spirulina platensis, and has been reported to have different functional properties, among them, the antioxidant. The objective of this study was to study the microencapsulation by spray-drying of phycocyanin, using the encapsulant maltodextrin, in stability tests and antioxidant potential in vitro and in vivo, in Saccharomyces cerevisiae cells. The results show that the encapsulation process produced spherical microcapsules of average size of 10 µm, with water solubility around 91.71%, water and moisture activity of 0.209 and 3.975%, respectively. Maltodextrin protected the active's physical agent temperature and promoted gradual release of the antioxidant potential and maintained the same after drying. In the in vivo tests the microcapsules statistically increased the enzymatic activity of glutathione and decreased levels of lipid peroxidation. It was concluded thatthe technique of encapsulating phycocyanin by spray dryer with the encapsulant maltodextrin was effective in maintaining the antioxidant activity of bioactive.O interesse na pesquisa de compostos bioativos tem aumentado, uma vez que os consumidores procuram produtos naturais e que resultem em benefícios para a sua saúde. A estabilidade desses compostos, na grande maioria, porém é de comportamento desconhecido.A utilização de técnicas que permitam garantir a funcionalidade desses ativos para diferentes fins torna-se muito importante. A encapsulação é uma tecnologia capaz de proteger o ativo das mais diversas condições de exposição, promover a liberação controlada, mascarar odor e sabor desagradáveis, além de promover a diluição homogênea do material encapsulado, em produtos alimentícios ou em formulações farmacêuticas. A ficocianina é uma ficobiliproteína, presente na cianobactéria Spirulina platensis, e vem sendo relatada por apresentar diferentes propriedades funcionais, dentre elas, a antioxidante. Objetiva-se estudar a microencapsulação por spray-drying da ficocianina, utilizando o encapsulante maltodextrina, em testes deestabilidade e potencial antioxidantein vitro e in vivo, em células de Saccharomyces cerevisiae. Os resultados mostram que o processo de encapsulamento produziu microcápsulas esféricas de tamanho médio de 10 ¿m, com solubilidade em água em torno de 91,71%, atividade de água e umidade de 0,209 e 3,975%, respectivamente. A maltodextrina protegeu o ativo do agente físico temperatura e promoveu liberação gradual do potencial antioxidante e manteve o mesmo após a secagem. Nos testes in vivo as microcápsulas aumentaram de forma estatisticamente significativa a atividade enzimática da glutationa e diminuiu os níveis de peroxidação lipídicaConclui-se que a técnica de encapsulamento da ficocianina por spray dryer, com o encapsulante maltodextrina foi eficaz para a manutenção da atividade antioxidante do bioativo.Universidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBrasilUPFPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosBertolin, Telma Elitahttp://lattes.cnpq.br/9808793425894633http://lattes.cnpq.br/4804304036455640Colla, Luciane MariaFormigheri, Mariana da Silva2025-05-07T00:34:22Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFORMIGHERI, Mariana da Silva. Estabilidade e potencial antioxidante de microcápsulas de ficocianina. 2017. 115 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade de Passo Fundo, Passo Fundo, RS, 2017.https://repositorio.upf.br/handle/123456789/1692porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-05-07T00:37:43Zoai:repositorio.upf.br:123456789/1692Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-05-07T00:37:43Repositório Institucional da UPF - Universidade de Passo Fundo (UPF)false
dc.title.none.fl_str_mv Estabilidade e potencial antioxidante de microcápsulas de ficocianina
Stability and antioxidant potential of phycocyanin microcapsules
title Estabilidade e potencial antioxidante de microcápsulas de ficocianina
spellingShingle Estabilidade e potencial antioxidante de microcápsulas de ficocianina
Formigheri, Mariana da Silva
Encapsulação
Funcionalidade
Spirulina platensis
CIENCIA E TECNOLOGIA DE ALIMENTOS::ENGENHARIA DE ALIMENTOS
title_short Estabilidade e potencial antioxidante de microcápsulas de ficocianina
title_full Estabilidade e potencial antioxidante de microcápsulas de ficocianina
title_fullStr Estabilidade e potencial antioxidante de microcápsulas de ficocianina
title_full_unstemmed Estabilidade e potencial antioxidante de microcápsulas de ficocianina
title_sort Estabilidade e potencial antioxidante de microcápsulas de ficocianina
author Formigheri, Mariana da Silva
author_facet Formigheri, Mariana da Silva
author_role author
dc.contributor.none.fl_str_mv Bertolin, Telma Elita
http://lattes.cnpq.br/9808793425894633
http://lattes.cnpq.br/4804304036455640
Colla, Luciane Maria
dc.contributor.author.fl_str_mv Formigheri, Mariana da Silva
dc.subject.por.fl_str_mv Encapsulação
Funcionalidade
Spirulina platensis
CIENCIA E TECNOLOGIA DE ALIMENTOS::ENGENHARIA DE ALIMENTOS
topic Encapsulação
Funcionalidade
Spirulina platensis
CIENCIA E TECNOLOGIA DE ALIMENTOS::ENGENHARIA DE ALIMENTOS
description Interest in bioactive compounds research has increased as consumers look for natural products that result in health benefits. The stability of these compounds, in the great majority, however is of unknown behavior. The use of techniques to ensure the functionality of these assets for different purposes becomes very important. Encapsulation is a technology capable of protecting the active substance from a wide range of exposure conditions, promoting controlled release, masking unpleasant odor and taste, and promoting the homogenous dilution of the encapsulated material in food products or pharmaceutical formulations. Phycocyanin is a phycobiliprotein, present in the cyanobacterium Spirulina platensis, and has been reported to have different functional properties, among them, the antioxidant. The objective of this study was to study the microencapsulation by spray-drying of phycocyanin, using the encapsulant maltodextrin, in stability tests and antioxidant potential in vitro and in vivo, in Saccharomyces cerevisiae cells. The results show that the encapsulation process produced spherical microcapsules of average size of 10 µm, with water solubility around 91.71%, water and moisture activity of 0.209 and 3.975%, respectively. Maltodextrin protected the active's physical agent temperature and promoted gradual release of the antioxidant potential and maintained the same after drying. In the in vivo tests the microcapsules statistically increased the enzymatic activity of glutathione and decreased levels of lipid peroxidation. It was concluded thatthe technique of encapsulating phycocyanin by spray dryer with the encapsulant maltodextrin was effective in maintaining the antioxidant activity of bioactive.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2025-05-07T00:34:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv FORMIGHERI, Mariana da Silva. Estabilidade e potencial antioxidante de microcápsulas de ficocianina. 2017. 115 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade de Passo Fundo, Passo Fundo, RS, 2017.
https://repositorio.upf.br/handle/123456789/1692
identifier_str_mv FORMIGHERI, Mariana da Silva. Estabilidade e potencial antioxidante de microcápsulas de ficocianina. 2017. 115 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade de Passo Fundo, Passo Fundo, RS, 2017.
url https://repositorio.upf.br/handle/123456789/1692
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade de Passo Fundo
Faculdade de Agronomia e Medicina Veterinária – FAMV
Brasil
UPF
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Universidade de Passo Fundo
Faculdade de Agronomia e Medicina Veterinária – FAMV
Brasil
UPF
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv reponame:Repositório Institucional da UPF
instname:Universidade de Passo Fundo (UPF)
instacron:UPF
instname_str Universidade de Passo Fundo (UPF)
instacron_str UPF
institution UPF
reponame_str Repositório Institucional da UPF
collection Repositório Institucional da UPF
repository.name.fl_str_mv Repositório Institucional da UPF - Universidade de Passo Fundo (UPF)
repository.mail.fl_str_mv jucelei@upf.br||biblio@upf.br
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