Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme

• Main Author: da Silva, Martin K.L. [UNESP]
• Publication Date: 2018
• Other Authors: Vanzela, Heloísa C. [UNESP], Defavari, Lucas M. [UNESP], Cesarino, Ivana [UNESP]
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNESP
• Download full: http://dx.doi.org/10.1016/j.snb.2018.09.051; http://hdl.handle.net/11449/171462
• topic: Acetylcholinesterase enzyme; Biosensor; Carbaryl; Food safety; Reduced graphene oxide

Food safety is a major concern for human health and wellbeing all over the world. A novel and sensitive biosensor based on reduced graphene oxide (rGO) and the enzyme acetylcholinesterase (AChE) was developed and applied for the detection of carbaryl in food samples. The glassy carbon/rGO/AChE biosensor was characterized morphologically and electrochemically using scanning electron microscopy and cyclic voltammetry/electrochemical impedance spectroscopy, respectively. Optimum differential pulse voltammetry conditions led to a nanomolar detection limit, and determination of carbaryl in tomato was achieved.