Influência de fatores ambientais e dos níveis de microrganismos potencialmente cariogênicos no desenvolvimento da cárie precoce da infância

• Main Author: Ribas, Lais Fernanda Fonseca [UNESP]
• Publication Date: 2015
• Format: Bachelor thesis
• Language: por
• Source: Repositório Institucional da UNESP
• Download full: http://hdl.handle.net/11449/149691; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-31/000867803.pdf
• topic: Cárie dentária; Streptococcus mutans; Streptococcus sobrinus; Lactobacillus; Dental caries

Early childhood caries (ECC) is a microbial infection that can severely affect the teeth of young children. The objectives of this study were to evaluate the influence of environmental factors (diet, hygiene, socioeconomic and cultural conditions) and the levels of potentially cariogenic microorganisms in the development of the ECC. 36 to 60 month-old children were selected to participate in this study. Children were grouped into: Caries Free group (CF, n=32), ECC group (n=25) and severe ECC group (S-ECC, n=33). Saliva and dental biofilm samples were collected from children to compare the levels of mutans streptococci and lactobacilli using culture method. Saliva was also evaluated to detect and quantify the following microorganisms: Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. by quantitative PCR (qPCR). Data about diet, oral hygiene, socioeconomic and cultural conditions were obtained by questionnaire and diet diary with the parents. Data were analyzed statistically considering p≤ 0.05. The results showed that S-ECC children had significantly lower income and lower educational level of their parents. Considering diet diary, children with S-ECC had longer breastfeeding and higher age at onset of artificial feeding. Microbiological data showed higher count of mutans streptococci in saliva of S-ECC children compared with the others groups. It was also noted by qPCR higher prevalence and quantity of S. mutans and S. sobrinus in saliva of S-ECC children. There was no difference in relation to lactobacilli levels for culture method and qPCR. It was concluded that there was influence of socio-economic and cultural risk factors, breastfeeding and levels of S. mutans and S. sobrinus in the severity of the ECC