Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression

Detalhes bibliográficos
Autor(a) principal: Silva, Walmir da
Data de Publicação: 2016
Outros Autores: Souza dos Santos, Robson Augusto, Moraes, Karen C. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11010-015-2598-5
http://hdl.handle.net/11449/161199
Resumo: The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.
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spelling Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expressionAngiotensin IICell culturemiRNAPost-transcriptional regulationPTENThe activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT-Nano-BiofarmaceuticaUniv Fed Ouro Preto, Nucleo Pesquisa Biol, Inst Ciencias Exatas & Biol, Lab Bioquim & Biol Mol, Ouro Preto, MG, BrazilUniv Fed Minas Gerais, Dept Fisiol & Biofis, Lab Fisiol, Belo Horizonte, MG, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Mol Biol Lab, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Mol Biol Lab, BR-13506900 Rio Claro, SP, BrazilFAPESP: FAPESP-2009/07671-2CNPq: 475586/2009-3SpringerUniv Fed Ouro PretoUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Silva, Walmir daSouza dos Santos, Robson AugustoMoraes, Karen C. M. [UNESP]2018-11-26T16:19:31Z2018-11-26T16:19:31Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article363-371application/pdfhttp://dx.doi.org/10.1007/s11010-015-2598-5Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016.0300-8177http://hdl.handle.net/11449/16119910.1007/s11010-015-2598-5WOS:000369808600036WOS000369808600036.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Biochemistry1,003info:eu-repo/semantics/openAccess2024-10-17T18:19:57Zoai:repositorio.unesp.br:11449/161199Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-17T18:19:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
title Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
spellingShingle Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
Silva, Walmir da
Angiotensin II
Cell culture
miRNA
Post-transcriptional regulation
PTEN
title_short Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
title_full Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
title_fullStr Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
title_full_unstemmed Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
title_sort Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
author Silva, Walmir da
author_facet Silva, Walmir da
Souza dos Santos, Robson Augusto
Moraes, Karen C. M. [UNESP]
author_role author
author2 Souza dos Santos, Robson Augusto
Moraes, Karen C. M. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Univ Fed Ouro Preto
Universidade Federal de Minas Gerais (UFMG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Walmir da
Souza dos Santos, Robson Augusto
Moraes, Karen C. M. [UNESP]
dc.subject.por.fl_str_mv Angiotensin II
Cell culture
miRNA
Post-transcriptional regulation
PTEN
topic Angiotensin II
Cell culture
miRNA
Post-transcriptional regulation
PTEN
description The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2018-11-26T16:19:31Z
2018-11-26T16:19:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11010-015-2598-5
Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016.
0300-8177
http://hdl.handle.net/11449/161199
10.1007/s11010-015-2598-5
WOS:000369808600036
WOS000369808600036.pdf
url http://dx.doi.org/10.1007/s11010-015-2598-5
http://hdl.handle.net/11449/161199
identifier_str_mv Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016.
0300-8177
10.1007/s11010-015-2598-5
WOS:000369808600036
WOS000369808600036.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular And Cellular Biochemistry
1,003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 363-371
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1834483690559766528

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