Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats

Bibliographic Details
Main Author: Costa, V. M. [UNESP]
Publication Date: 2010
Other Authors: Langoni, H. [UNESP]
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.1590/S1678-91992010000200016
http://hdl.handle.net/11449/219571
Summary: The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
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spelling Detection of Toxoplasma gondii in the milk of experimentally infected wistar female ratsBioassayFemale ratsOxoplasma gondiiParasite in milkPCRThe milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.Zoonosis Research Center NUPEZO Veterinary Medicine and Animal Husbandry School S̃o Paulo State University (UNESP-Univ Estadual Paulista), Botucatu, São Paulo StateZoonosis Research Center NUPEZO Veterinary Medicine and Animal Husbandry School S̃o Paulo State University (UNESP-Univ Estadual Paulista), Botucatu, São Paulo StateUniversidade Estadual Paulista (UNESP)Costa, V. M. [UNESP]Langoni, H. [UNESP]2022-04-28T18:56:17Z2022-04-28T18:56:17Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374http://dx.doi.org/10.1590/S1678-91992010000200016Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 2, p. 368-374, 2010.1678-9199http://hdl.handle.net/11449/21957110.1590/S1678-919920100002000162-s2.0-77954158937Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T18:56:17Zoai:repositorio.unesp.br:11449/219571Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-03-28T14:47:39.990868Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
title Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
spellingShingle Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
Costa, V. M. [UNESP]
Bioassay
Female rats
Oxoplasma gondii
Parasite in milk
PCR
title_short Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
title_full Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
title_fullStr Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
title_full_unstemmed Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
title_sort Detection of Toxoplasma gondii in the milk of experimentally infected wistar female rats
author Costa, V. M. [UNESP]
author_facet Costa, V. M. [UNESP]
Langoni, H. [UNESP]
author_role author
author2 Langoni, H. [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Costa, V. M. [UNESP]
Langoni, H. [UNESP]
dc.subject.por.fl_str_mv Bioassay
Female rats
Oxoplasma gondii
Parasite in milk
PCR
topic Bioassay
Female rats
Oxoplasma gondii
Parasite in milk
PCR
description The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2022-04-28T18:56:17Z
2022-04-28T18:56:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992010000200016
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 2, p. 368-374, 2010.
1678-9199
http://hdl.handle.net/11449/219571
10.1590/S1678-91992010000200016
2-s2.0-77954158937
url http://dx.doi.org/10.1590/S1678-91992010000200016
http://hdl.handle.net/11449/219571
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 2, p. 368-374, 2010.
1678-9199
10.1590/S1678-91992010000200016
2-s2.0-77954158937
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 368-374
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1834484025791610880

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