Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro
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| Publication Date: | 2019 |
| Format: | Master thesis |
| Language: | por |
| Source: | Biblioteca Digital de Teses e Dissertações da UFTM |
| Download full: | http://bdtd.uftm.edu.br/handle/tede/857 |
Summary: | Protocols for treatment of oral squamous cell carcinoma cause numerous adverse effects, which may interfere to important functions such as nutrition, phonation and respiration. Natural compounds has been studied for the treatment of cancer. Annona muricata has been used for medicinal purposes for years however its participation in the inhibition of tumorigenesis has not yet been fully elucidated. This study aimed to evaluate the effect of Annona muricata extracts on cytotoxicity and cell proliferation of SCC-4 cells of oral squamous cell carcinoma in vitro. Phytochemical screening was performed for the detection of tannins, flavonol/flavones and alkaloids at 1 mg/mL. The IC50 of extracts: chloroform (EC), ethanolic (EE) e methanolic (EM) was determinate by the Neutral Red incorporation assay for 24 h. The percentage of proliferative cells was quantified by BrdU incorporation assay at concentrations below the IC50 for 6 h. A significance of p<0.05 was considered. The quantity of tannins in EC was lower than in EE and EM (p<0.0001) and the flavonol and flavones content in EC was higher than EE and EM (p<0.001). However, the amount of tannins. However, the amout of tannins, flavonol and flavones was similar in EE and EM. No alkaloids were detected in the extracts studied by using this method. EC, EE and EM extracts were cytotoxic to SCC-4 cells, with IC50 of 6.48, 46.52 and 20.38 μg/mL, respectively. The percentage of proliferative cells was significantly lower in EC, EE and EM than the vehicle control, corresponding to an inhibition of the cell proliferation rate of 19%, 38% and, 38% respectively. These results suggest that chloroform, ethanolic and methanolic extracts of Annona muricata have a cytotoxic and antiproliferative effect on SCC-4 cells of oral squamous cell carcinoma. |
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CREMA, Virgínia Oliveirahttp://lattes.cnpq.br/2599388555203811CARNEIRO, Anna Cecília Dias Macielhttp://lattes.cnpq.br/4647988258183696HISS, Lorraine Stephane2019-09-06T13:23:06Z2019-08-06HISS, Lorraine Stephane. Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro. 2019. 78f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2019.http://bdtd.uftm.edu.br/handle/tede/857Protocols for treatment of oral squamous cell carcinoma cause numerous adverse effects, which may interfere to important functions such as nutrition, phonation and respiration. Natural compounds has been studied for the treatment of cancer. Annona muricata has been used for medicinal purposes for years however its participation in the inhibition of tumorigenesis has not yet been fully elucidated. This study aimed to evaluate the effect of Annona muricata extracts on cytotoxicity and cell proliferation of SCC-4 cells of oral squamous cell carcinoma in vitro. Phytochemical screening was performed for the detection of tannins, flavonol/flavones and alkaloids at 1 mg/mL. The IC50 of extracts: chloroform (EC), ethanolic (EE) e methanolic (EM) was determinate by the Neutral Red incorporation assay for 24 h. The percentage of proliferative cells was quantified by BrdU incorporation assay at concentrations below the IC50 for 6 h. A significance of p<0.05 was considered. The quantity of tannins in EC was lower than in EE and EM (p<0.0001) and the flavonol and flavones content in EC was higher than EE and EM (p<0.001). However, the amount of tannins. However, the amout of tannins, flavonol and flavones was similar in EE and EM. No alkaloids were detected in the extracts studied by using this method. EC, EE and EM extracts were cytotoxic to SCC-4 cells, with IC50 of 6.48, 46.52 and 20.38 μg/mL, respectively. The percentage of proliferative cells was significantly lower in EC, EE and EM than the vehicle control, corresponding to an inhibition of the cell proliferation rate of 19%, 38% and, 38% respectively. These results suggest that chloroform, ethanolic and methanolic extracts of Annona muricata have a cytotoxic and antiproliferative effect on SCC-4 cells of oral squamous cell carcinoma.Os protocolos de tratamento de carcinoma epidermoide oral causam numerosos efeitos adversos, podendo dificultar funções importantes como: nutrição, fonação e respiração. Os compostos naturais têm sido estudados para o tratamento do câncer. A Annona muricata é usada com finalidade medicinal por anos, no entanto, sua participação na inibição da tumorigênese ainda não está completamente elucidada. Este estudo visou avaliar o efeito de extratos de Annona muricata sobre citotoxicidade e a proliferação de células SCC-4 de carcinoma epidermoide oral in vitro. Foi realizado screening fitoquímico para detecção de taninos, flavonois/flavonas e alcaloides a 1 mg/mL. O IC50 dos extratos: clorofórmico (EC), etanólico (EE) e metanólico (EM) foi determinado por meio de ensaio de incorporação do Vermelho Neutro por 24 h. Foi quantificado o percentual de células proliferativas por meio de ensaio de incorporação do Brdu em concentrações abaixo do IC50 por 6 h. Foi considerada uma significância de p<0,05. A quantidade de taninos em EC foi menor que em EE e EM (p<0,0001) e o teor de flavonois e flavonas em EC foi maior que EE e EM (p<0,001). No entanto, a quantidade de taninos, flavonois e flavonas foi similar em EE e EM. Não foram detectados alcaloides nos extratos estudados. Os extratos EC, EE e EM foram citotóxicos para células SCC-4, apresentando IC50 de 6,48, 46,52 e 20,38 μg/mL, respectivamente. O percentual de células proliferativas foi significativamente menor em EC, EE e EM que o controle veículo, correspondendo a uma inibição da taxa proliferação celular de: 19%, 38% e 38%, respectivamente. Os resultados sugerem que os extratos clorofórmico, etanólico e metanólico de Annona muricata têm efeito citotóxico e antiproliferativo em células SCC-4 de carcinoma epidermoide oral.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal do Triêngulo Mineiroapplication/pdfhttp://bdtd.uftm.edu.br/retrieve/5687/Dissert%20Lorraine%20S%20Hiss.pdf.jpgporUniversidade Federal do Triângulo MineiroPrograma de Pós-Graduação em Ciências da SaúdeUFTMBrasilInstituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da SaúdeABDUL WAHAB, S. M. et al. 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| dc.title.por.fl_str_mv |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| title |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| spellingShingle |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro HISS, Lorraine Stephane Annona muricata. Carcinoma epidermoide oral. Citotoxicidade. Proliferação celular. Células SCC-4. Annona muricata. Cell proliferation. Cytotoxicity. Oral squamous cell carcinoma. SCC-4 cells. Citologia e Biologia Celular |
| title_short |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| title_full |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| title_fullStr |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| title_full_unstemmed |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| title_sort |
Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro |
| author |
HISS, Lorraine Stephane |
| author_facet |
HISS, Lorraine Stephane |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
CREMA, Virgínia Oliveira |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2599388555203811 |
| dc.contributor.advisor-co1.fl_str_mv |
CARNEIRO, Anna Cecília Dias Maciel |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4647988258183696 |
| dc.contributor.author.fl_str_mv |
HISS, Lorraine Stephane |
| contributor_str_mv |
CREMA, Virgínia Oliveira CARNEIRO, Anna Cecília Dias Maciel |
| dc.subject.por.fl_str_mv |
Annona muricata. Carcinoma epidermoide oral. Citotoxicidade. Proliferação celular. Células SCC-4. |
| topic |
Annona muricata. Carcinoma epidermoide oral. Citotoxicidade. Proliferação celular. Células SCC-4. Annona muricata. Cell proliferation. Cytotoxicity. Oral squamous cell carcinoma. SCC-4 cells. Citologia e Biologia Celular |
| dc.subject.eng.fl_str_mv |
Annona muricata. Cell proliferation. Cytotoxicity. Oral squamous cell carcinoma. SCC-4 cells. |
| dc.subject.cnpq.fl_str_mv |
Citologia e Biologia Celular |
| description |
Protocols for treatment of oral squamous cell carcinoma cause numerous adverse effects, which may interfere to important functions such as nutrition, phonation and respiration. Natural compounds has been studied for the treatment of cancer. Annona muricata has been used for medicinal purposes for years however its participation in the inhibition of tumorigenesis has not yet been fully elucidated. This study aimed to evaluate the effect of Annona muricata extracts on cytotoxicity and cell proliferation of SCC-4 cells of oral squamous cell carcinoma in vitro. Phytochemical screening was performed for the detection of tannins, flavonol/flavones and alkaloids at 1 mg/mL. The IC50 of extracts: chloroform (EC), ethanolic (EE) e methanolic (EM) was determinate by the Neutral Red incorporation assay for 24 h. The percentage of proliferative cells was quantified by BrdU incorporation assay at concentrations below the IC50 for 6 h. A significance of p<0.05 was considered. The quantity of tannins in EC was lower than in EE and EM (p<0.0001) and the flavonol and flavones content in EC was higher than EE and EM (p<0.001). However, the amount of tannins. However, the amout of tannins, flavonol and flavones was similar in EE and EM. No alkaloids were detected in the extracts studied by using this method. EC, EE and EM extracts were cytotoxic to SCC-4 cells, with IC50 of 6.48, 46.52 and 20.38 μg/mL, respectively. The percentage of proliferative cells was significantly lower in EC, EE and EM than the vehicle control, corresponding to an inhibition of the cell proliferation rate of 19%, 38% and, 38% respectively. These results suggest that chloroform, ethanolic and methanolic extracts of Annona muricata have a cytotoxic and antiproliferative effect on SCC-4 cells of oral squamous cell carcinoma. |
| publishDate |
2019 |
| dc.date.accessioned.fl_str_mv |
2019-09-06T13:23:06Z |
| dc.date.issued.fl_str_mv |
2019-08-06 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
HISS, Lorraine Stephane. Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro. 2019. 78f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2019. |
| dc.identifier.uri.fl_str_mv |
http://bdtd.uftm.edu.br/handle/tede/857 |
| identifier_str_mv |
HISS, Lorraine Stephane. Extratos de folhas de annona muricata são citotóxicos e reduzem a proliferação celular em carcinoma epidermóide oral in vitro. 2019. 78f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2019. |
| url |
http://bdtd.uftm.edu.br/handle/tede/857 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.references.por.fl_str_mv |
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The chemopotential effect of Annona muricata leaves against azoxymethane-induced colonic aberrant crypt foci in rats and the apoptotic effect of Acetogenin Annomuricin E in HT-29 cells: a bioassayguided approach. PLoS One, v. 10, p. e0122288, 2015. ZUANAZZI, J. A. S.; MONTANHA, J. A. Flavonóides. In Simões CMO (org.). Farmacognosia: da planta ao medicamento. Porto Alegre: UFRGS/Ed. UFSC. 2004.p. 577-614.7 |
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