Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii

• Main Author: Castro, Verônica Souza Paiva
• Publication Date: 2011
• Format: Master thesis
• Language: por
• Source: Manancial - Repositório Digital da UFSM
• dARK ID: ark:/26339/0013000000qzs
• Download full: http://repositorio.ufsm.br/handle/1/10109
• topic: Esporotricose; Adenosina desaminase; Linfócitos; Soro; Sporotrichosis; Adenosine deaminase; Lymphocytes; Serum; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Sporotrichosis is a subcutaneous fungal infection of evolution subacute or chronic, inflammatory lesions characterized by pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a key enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions, however, no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and ADA-L were performed on days 15, 30 and 40 post-infection (PI) to assess disease progression. In experiment II, was observed in an acute decrease in activity of S-ADA and L-ADA (p <0.05), suggesting a compensatory mechanism in the body's attempt to protect the host from excessive tissue damage. Chronicity of the disease the rats in the experiment I and II at 30 days PI, showed an increased activity of L-ADA (p <0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis.