Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)

Bibliographic Details
Main Author: Aline Gonzaga Cunha
Publication Date: 2015
Format: Master thesis
Language: por
Source: Biblioteca Digital de Teses e Dissertações da UFC
Download full: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14463
Summary: The dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisActivity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)Atividade de enzimas da via de sÃntese dos fenÃlicos durante o desenvolvimento de pedÃnculos de clones de cajueiro (Anacardium occidentale L.)2015-02-25Maria Raquel AlcÃntara de Miranda46142371349http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4796542P9Edy Sousa de Brito56506155434http://lattes.cnpq.br/5168806776816009JesÃs Fernando Ayala Zavalag02244079nÃo informado01145805370http://lattes.cnpq.br/6530301060914589Aline Gonzaga CunhaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em BioquÃmicaUFCBRCompostos fenÃlicos PAL UGT FLS LAR EstÃdios de maturaÃÃo Cromatografia lÃquidaPhenolic compounds Ripening stages Liquid chromatographyBIOQUIMICAThe dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health. O cajueiro (Anacardium occidentale L.) Ã uma planta tropical, originÃria do Brasil, que se destaca como espÃcie frutÃfera com elevada potencialidade para o consumo in natura e para o processamento industrial. Com a descoberta de compostos bioativos presentes nos pedÃnculos, se fazem necessÃrios estudos da atividade de enzimas da sÃntese dos compostos fenÃlicos, tais como a fenilalanina amÃnia liase (PAL), UDP-glicose: trans-cinamato β-D- glicosiltransferase (UGT), flavonol sintase (FLS) e leucoantocianidina redutase (LAR). Logo, esse estudo objetivou determinar a atividade dessas enzimas, em pseudofrutos de cajueiro-anÃo-precoce dos clones CCP 76 e BRS 189 em trÃs diferentes estÃdios de maturaÃÃo, alÃm de estabelecer o perfil de fenÃlicos nestes referidos estÃdios. Os produtos resultantes das reaÃÃes enzimÃticas foram identificados e quantificados por LC-DAD. A atividade da PAL decresce com o amadurecimento dos pseudofrutos. A enzima UGT no estÃdio 4 do clone CCP 76 apresentou a maior atividade enzimÃtica especÃfica que nos outros estÃdios de desenvolvimento para o referido clone. Enquanto no clone BRS 189, a maior atividade deteve-se no caju maduro. A maior sÃntese de quercetina no caju foi evidenciada no estÃdio 4 para os dois clones estudados. As enzimas envolvidas no metabolismo de fenÃlicos se comportam diferentemente, demonstrando a complexidade dessa rota biossintÃtica que varia entre os clones e durante o desenvolvimento dos pedÃnculos de cajueiro. Estudos posteriores, com CLAE acoplada a espectrÃmetro de massa, irÃo elucidar melhor o perfil dos compostos presentes em cajus liofilizados para caraterizaÃÃo quÃmica destes em diferentes estÃdios de maturaÃÃo. Portanto, o entendimento do metabolismo enzimÃtico entre os clones e durante o desenvolvimento do pedÃnculo representam um potencial real para o desenvolvimento de novos produtos com propriedades funcionais e promoÃÃo da saÃde humana. Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicohttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14463application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:27:48Zmail@mail.com -
dc.title.en.fl_str_mv Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
dc.title.alternative.pt.fl_str_mv Atividade de enzimas da via de sÃntese dos fenÃlicos durante o desenvolvimento de pedÃnculos de clones de cajueiro (Anacardium occidentale L.)
title Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
spellingShingle Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
Aline Gonzaga Cunha
Compostos fenÃlicos
PAL
UGT
FLS
LAR
EstÃdios de maturaÃÃo
Cromatografia lÃquida
Phenolic compounds
Ripening stages
Liquid chromatography
BIOQUIMICA
title_short Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
title_full Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
title_fullStr Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
title_full_unstemmed Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
title_sort Activity of phenolic synthesis pathway enzymes during the development of peduncles clones cashew (Anacardium occidentale L.)
author Aline Gonzaga Cunha
author_facet Aline Gonzaga Cunha
author_role author
dc.contributor.advisor1.fl_str_mv Maria Raquel AlcÃntara de Miranda
dc.contributor.advisor1ID.fl_str_mv 46142371349
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4796542P9
dc.contributor.referee1.fl_str_mv Edy Sousa de Brito
dc.contributor.referee1ID.fl_str_mv 56506155434
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5168806776816009
dc.contributor.referee2.fl_str_mv JesÃs Fernando Ayala Zavala
dc.contributor.referee2ID.fl_str_mv g02244079
dc.contributor.referee2Lattes.fl_str_mv nÃo informado
dc.contributor.authorID.fl_str_mv 01145805370
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6530301060914589
dc.contributor.author.fl_str_mv Aline Gonzaga Cunha
contributor_str_mv Maria Raquel AlcÃntara de Miranda
Edy Sousa de Brito
JesÃs Fernando Ayala Zavala
dc.subject.por.fl_str_mv Compostos fenÃlicos
PAL
UGT
FLS
LAR
EstÃdios de maturaÃÃo
Cromatografia lÃquida
topic Compostos fenÃlicos
PAL
UGT
FLS
LAR
EstÃdios de maturaÃÃo
Cromatografia lÃquida
Phenolic compounds
Ripening stages
Liquid chromatography
BIOQUIMICA
dc.subject.eng.fl_str_mv Phenolic compounds
Ripening stages
Liquid chromatography
dc.subject.cnpq.fl_str_mv BIOQUIMICA
dc.description.sponsorship.fl_txt_mv Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
dc.description.abstract.por.fl_txt_mv The dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health.
O cajueiro (Anacardium occidentale L.) Ã uma planta tropical, originÃria do Brasil, que se destaca como espÃcie frutÃfera com elevada potencialidade para o consumo in natura e para o processamento industrial. Com a descoberta de compostos bioativos presentes nos pedÃnculos, se fazem necessÃrios estudos da atividade de enzimas da sÃntese dos compostos fenÃlicos, tais como a fenilalanina amÃnia liase (PAL), UDP-glicose: trans-cinamato β-D- glicosiltransferase (UGT), flavonol sintase (FLS) e leucoantocianidina redutase (LAR). Logo, esse estudo objetivou determinar a atividade dessas enzimas, em pseudofrutos de cajueiro-anÃo-precoce dos clones CCP 76 e BRS 189 em trÃs diferentes estÃdios de maturaÃÃo, alÃm de estabelecer o perfil de fenÃlicos nestes referidos estÃdios. Os produtos resultantes das reaÃÃes enzimÃticas foram identificados e quantificados por LC-DAD. A atividade da PAL decresce com o amadurecimento dos pseudofrutos. A enzima UGT no estÃdio 4 do clone CCP 76 apresentou a maior atividade enzimÃtica especÃfica que nos outros estÃdios de desenvolvimento para o referido clone. Enquanto no clone BRS 189, a maior atividade deteve-se no caju maduro. A maior sÃntese de quercetina no caju foi evidenciada no estÃdio 4 para os dois clones estudados. As enzimas envolvidas no metabolismo de fenÃlicos se comportam diferentemente, demonstrando a complexidade dessa rota biossintÃtica que varia entre os clones e durante o desenvolvimento dos pedÃnculos de cajueiro. Estudos posteriores, com CLAE acoplada a espectrÃmetro de massa, irÃo elucidar melhor o perfil dos compostos presentes em cajus liofilizados para caraterizaÃÃo quÃmica destes em diferentes estÃdios de maturaÃÃo. Portanto, o entendimento do metabolismo enzimÃtico entre os clones e durante o desenvolvimento do pedÃnculo representam um potencial real para o desenvolvimento de novos produtos com propriedades funcionais e promoÃÃo da saÃde humana.
description The dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14463
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14463
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em BioquÃmica
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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