Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen

Bibliographic Details
Main Author: Rebouças, Miriam Flores
Publication Date: 2013
Other Authors: Loureiro, Dan, Bastos, Bruno Lopes, Costa, Lilia F. Moura, Hanna, Samira A., Azevedo, Vasco Ariston de Carvalho, Nascimento, Roberto José Meyer, Portela, Ricardo Wagner Dias
Format: Article
Language: eng
Source: Repositório Institucional da UFBA
Download full: http://repositorio.ufba.br/ri/handle/ri/14889
Summary: p. 1296-1302
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spelling Rebouças, Miriam FloresLoureiro, DanBastos, Bruno LopesCosta, Lilia F. MouraHanna, Samira A.Azevedo, Vasco Ariston de CarvalhoNascimento, Roberto José MeyerPortela, Ricardo Wagner Dias2014-05-01T13:00:49Z2014-05-01T13:00:49Z20130100-736Xhttp://repositorio.ufba.br/ri/handle/ri/14889v. 33, n. 11p. 1296-1302Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CLA), a chronic disease that affects goats and sheep, characterized by granuloma formation in subcutaneous and internal lymph nodes. CLA causes significant economic losses to commercial goat herds. In this study, we aimed to test secreted antigens secreted from T1 strain bacteria grown in brain heart infusion (BHI) broth in an indirect ELISA system to determine the presence of specific immunoglobulins against C. pseudotuberculosis. We analyzed the BHI antigen electrophoretic profile and the recognition pattern by infected sheep sera samples. The ELISA results were compared with multiplex PCR assay and IFN-gamma production. The ELISA was able to discriminate between negative and positive animals, with a sensitivity of 89% and a specificity of 99%, using microbiological isolation as gold standard. When this assay was compared with multiplex PCR and specific IFN-gamma quantification, six discrepant results were found among thirty-two samples. We concluded that the ELISA using antigens secreted from C. pseudotuberculosis T1 strain growth in BHI broth culture can be used for the serodiagnosis of CLA in sheep.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-10T12:59:01Z No. of bitstreams: 1 0100-736X2013001100002.pdf: 574041 bytes, checksum: e063fcebe5f6745a1df8fa19ce6acac0 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-05-01T13:00:49Z (GMT) No. of bitstreams: 1 0100-736X2013001100002.pdf: 574041 bytes, checksum: e063fcebe5f6745a1df8fa19ce6acac0 (MD5)Made available in DSpace on 2014-05-01T13:00:49Z (GMT). No. of bitstreams: 1 0100-736X2013001100002.pdf: 574041 bytes, checksum: e063fcebe5f6745a1df8fa19ce6acac0 (MD5) Previous issue date: 2013http://dx.doi.org/10.1590/S0100-736X2013001100002reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACaseous lymphadenitisCorynebacterium pseudotuberculosisSheepELISADevelopment of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigenPesquisa Veterinária Brasileirainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINAL0100-736X2013001100002.pdf0100-736X2013001100002.pdfapplication/pdf574041https://repositorio.ufba.br/bitstream/ri/14889/1/0100-736X2013001100002.pdfe063fcebe5f6745a1df8fa19ce6acac0MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14889/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT0100-736X2013001100002.pdf.txt0100-736X2013001100002.pdf.txtExtracted texttext/plain39771https://repositorio.ufba.br/bitstream/ri/14889/3/0100-736X2013001100002.pdf.txt2c77cc7c37dacb1283556010c08da35dMD53ri/148892022-07-01 10:46:39.581oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestrepositorio@ufba.bropendoar:19322022-07-01T13:46:39Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
dc.title.alternative.pt_BR.fl_str_mv Pesquisa Veterinária Brasileira
title Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
spellingShingle Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
Rebouças, Miriam Flores
Caseous lymphadenitis
Corynebacterium pseudotuberculosis
Sheep
ELISA
title_short Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
title_full Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
title_fullStr Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
title_full_unstemmed Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
title_sort Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen
author Rebouças, Miriam Flores
author_facet Rebouças, Miriam Flores
Loureiro, Dan
Bastos, Bruno Lopes
Costa, Lilia F. Moura
Hanna, Samira A.
Azevedo, Vasco Ariston de Carvalho
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
author_role author
author2 Loureiro, Dan
Bastos, Bruno Lopes
Costa, Lilia F. Moura
Hanna, Samira A.
Azevedo, Vasco Ariston de Carvalho
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rebouças, Miriam Flores
Loureiro, Dan
Bastos, Bruno Lopes
Costa, Lilia F. Moura
Hanna, Samira A.
Azevedo, Vasco Ariston de Carvalho
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
dc.subject.por.fl_str_mv Caseous lymphadenitis
Corynebacterium pseudotuberculosis
Sheep
ELISA
topic Caseous lymphadenitis
Corynebacterium pseudotuberculosis
Sheep
ELISA
description p. 1296-1302
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-01T13:00:49Z
dc.date.available.fl_str_mv 2014-05-01T13:00:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14889
dc.identifier.issn.none.fl_str_mv 0100-736X
dc.identifier.number.pt_BR.fl_str_mv v. 33, n. 11
identifier_str_mv 0100-736X
v. 33, n. 11
url http://repositorio.ufba.br/ri/handle/ri/14889
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2013001100002
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/14889/1/0100-736X2013001100002.pdf
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