In vitro and in vivo activity of a new unsymmetrical dinuclear copper complex containing a derivative ligand of 1,4,7-triazacyclononane: Catalytic promiscuity of [Cu2(L)Cl3]

• Main Author: De Almeida V.R.
• Publication Date: 2013
• Other Authors: Osorio R.E.H.M.B., Xavier, Fernando Roberto, Bessa L.M., Schilling E.L., Costa T.G., Bortolotto T., Cavalett A., Castro F.A.V., Vilhena F., Alves O.C., Terenzi H., Eleutherio E.C.A., Pereira M.D., Haase W., Tomkowicz Z., Szpoganicz B., Bortoluzzi A.J., Neves A.
• Format: Article
• Language: eng
• Source: Repositório Institucional da Udesc
• dARK ID: ark:/33523/001300000j021
• Download full: https://repositorio.udesc.br/handle/UDESC/8899

Here we present the synthesis of the dinuclear complex [Cu II2(L)Cl3] (1), where L is the deprotonated form of the 3-[(4,7-diisopropyl-1,4,7-triazacyclononan-1-yl)methyl]-2-hydroxy-5- methylbenzaldehyde ligand. The complex was characterized by single crystal X-ray diffraction, potentiometric titration, mass spectrometry, electrochemical and magnetic measurements, EPR, UV-Vis and IR. Complex 1 is able to increase the hydrolysis rate of the diester bis-(2,4-dinitrophenyl)phosphate (2,4-BDNPP) by a factor of 2700, and also to promote the plasmidial DNA cleavage at pH 6 and to inhibit the formazan chromophore formation in redox processes at pH 7. Using Saccharomyces cerevisiae (BY4741) as a eukaryotic cellular model, we observed that 1 presents reduced cytotoxicity. In addition, treatment of wild-type and mutant cells lacking Cu/Zn-superoxide dismutase (Sod1) and cytoplasmic catalase (Ctt1) with 1 promotes increased survival after H2O2 or menadione (O2- generator) stress, indicating that 1 might act as a Sod1 and Ctt1 mimetic. Considered together, these results support considerations regarding the dynamic behaviour of an unsymmetrical dinuclear copper(ii) complex in solid state and in aqueous pH-dependent solution. © The Royal Society of Chemistry 2013.