Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus

Bibliographic Details
Main Author: Rodrigues,Diogenes
Publication Date: 2013
Other Authors: de-Paris,Fernanda, Paiva,Rodrigo Minuto
Format: Article
Language: eng
Source: Revista da Sociedade Brasileira de Medicina Tropical
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625
Summary: Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.
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spelling Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virusHerpes simplex virusVaricella zoster virusPolymerase chain reaction Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. Sociedade Brasileira de Medicina Tropical - SBMT2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625Revista da Sociedade Brasileira de Medicina Tropical v.46 n.5 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-1520-2013info:eu-repo/semantics/openAccessRodrigues,Diogenesde-Paris,FernandaPaiva,Rodrigo Minutoeng2013-11-22T00:00:00Zoai:scielo:S0037-86822013000500625Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-11-22T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
spellingShingle Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Rodrigues,Diogenes
Herpes simplex virus
Varicella zoster virus
Polymerase chain reaction
title_short Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_full Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_fullStr Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_full_unstemmed Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_sort Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
author Rodrigues,Diogenes
author_facet Rodrigues,Diogenes
de-Paris,Fernanda
Paiva,Rodrigo Minuto
author_role author
author2 de-Paris,Fernanda
Paiva,Rodrigo Minuto
author2_role author
author
dc.contributor.author.fl_str_mv Rodrigues,Diogenes
de-Paris,Fernanda
Paiva,Rodrigo Minuto
dc.subject.por.fl_str_mv Herpes simplex virus
Varicella zoster virus
Polymerase chain reaction
topic Herpes simplex virus
Varicella zoster virus
Polymerase chain reaction
description Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-1520-2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.46 n.5 2013
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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