Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats

Detalhes bibliográficos
Autor(a) principal: Chagas,Carlos Eduardo Andrade
Data de Publicação: 2009
Outros Autores: Vieira,Alessandra, Ong,Thomas Prates, Moreno,Fernando Salvador
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500007
Resumo: PURPOSE: To study farnesol (FOH) effects on liver regeneration after 70% partial hepatectomy (PH) in rats. METHODS: Animals received FOH (25 mg/100 g body weight/day) or corn oil (CO, 0.25 mL/100 g body weight/day, controls). After a 2 week-treatment, all animals were subjected to PH and euthanized at different time points (0 h, 0.5 h, 4 h, 8 h, 18 h and 24 h) after surgery. Hepatic cell proliferation (PCNA positive nuclei) and apoptosis (fluorescence microscopy) were evaluated. RESULTS: Compared to CO treatment, FOH treatment inhibited (p<0.05) cell proliferation at 24h (S phase of the cell cycle) after PH. This was preceded by an induction of apoptosis 0.5 h (p<0.05; G0/G1 transition phase) after surgery. CONCLUSION: The results of the present study suggest that apoptosis induction could be associated with the reduced number of cells at the S phase observed in FOH group. These novel in vivo data reinforce FOH as a promising chemopreventive and therapeutic agent against cancer.
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spelling Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in ratsFarnesolHepatectomyCell ProliferationApoptosisRatsPURPOSE: To study farnesol (FOH) effects on liver regeneration after 70% partial hepatectomy (PH) in rats. METHODS: Animals received FOH (25 mg/100 g body weight/day) or corn oil (CO, 0.25 mL/100 g body weight/day, controls). After a 2 week-treatment, all animals were subjected to PH and euthanized at different time points (0 h, 0.5 h, 4 h, 8 h, 18 h and 24 h) after surgery. Hepatic cell proliferation (PCNA positive nuclei) and apoptosis (fluorescence microscopy) were evaluated. RESULTS: Compared to CO treatment, FOH treatment inhibited (p<0.05) cell proliferation at 24h (S phase of the cell cycle) after PH. This was preceded by an induction of apoptosis 0.5 h (p<0.05; G0/G1 transition phase) after surgery. CONCLUSION: The results of the present study suggest that apoptosis induction could be associated with the reduced number of cells at the S phase observed in FOH group. These novel in vivo data reinforce FOH as a promising chemopreventive and therapeutic agent against cancer.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500007Acta Cirúrgica Brasileira v.24 n.5 2009reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502009000500007info:eu-repo/semantics/openAccessChagas,Carlos Eduardo AndradeVieira,AlessandraOng,Thomas PratesMoreno,Fernando Salvadoreng2009-10-15T00:00:00Zoai:scielo:S0102-86502009000500007Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2009-10-15T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
title Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
spellingShingle Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
Chagas,Carlos Eduardo Andrade
Farnesol
Hepatectomy
Cell Proliferation
Apoptosis
Rats
title_short Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
title_full Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
title_fullStr Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
title_full_unstemmed Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
title_sort Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats
author Chagas,Carlos Eduardo Andrade
author_facet Chagas,Carlos Eduardo Andrade
Vieira,Alessandra
Ong,Thomas Prates
Moreno,Fernando Salvador
author_role author
author2 Vieira,Alessandra
Ong,Thomas Prates
Moreno,Fernando Salvador
author2_role author
author
author
dc.contributor.author.fl_str_mv Chagas,Carlos Eduardo Andrade
Vieira,Alessandra
Ong,Thomas Prates
Moreno,Fernando Salvador
dc.subject.por.fl_str_mv Farnesol
Hepatectomy
Cell Proliferation
Apoptosis
Rats
topic Farnesol
Hepatectomy
Cell Proliferation
Apoptosis
Rats
description PURPOSE: To study farnesol (FOH) effects on liver regeneration after 70% partial hepatectomy (PH) in rats. METHODS: Animals received FOH (25 mg/100 g body weight/day) or corn oil (CO, 0.25 mL/100 g body weight/day, controls). After a 2 week-treatment, all animals were subjected to PH and euthanized at different time points (0 h, 0.5 h, 4 h, 8 h, 18 h and 24 h) after surgery. Hepatic cell proliferation (PCNA positive nuclei) and apoptosis (fluorescence microscopy) were evaluated. RESULTS: Compared to CO treatment, FOH treatment inhibited (p<0.05) cell proliferation at 24h (S phase of the cell cycle) after PH. This was preceded by an induction of apoptosis 0.5 h (p<0.05; G0/G1 transition phase) after surgery. CONCLUSION: The results of the present study suggest that apoptosis induction could be associated with the reduced number of cells at the S phase observed in FOH group. These novel in vivo data reinforce FOH as a promising chemopreventive and therapeutic agent against cancer.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502009000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.24 n.5 2009
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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