Toxicity evaluation of herbicides used in sugarcane crops to Zebrafish (Danio rerio): DOI: 10.15343/0104-7809.20143801086097

• Main Author: Sanches Tesolin, Gabrielli Aparecida
• Publication Date: 2014
• Other Authors: Marina Menezes Marson, Martín Jonsson, Claudio, Arsénia Nogueira, António José, Andrade de Siqueira Franco, Daniel, Batista de Almeida, Sydnei Dionísio, Barifouse Matallo, Marcus, Accaui Marcondes de Moura, Monica
• Format: Article
• Language: por
• Source: O Mundo da Saúde (Online)
• Download full: https://revistamundodasaude.emnuvens.com.br/mundodasaude/article/view/405
• topic: Herbicidas - toxicidade. Diurona. Toxicidade Aguda. Misturas Complexas.; Herbicides - toxicity. Diuron. Acute Toxicity. Complex Mixtures.

In nature, organisms are constantly exposed to more than one toxic agent, and although the phenomenon of chemical interactionsare known for some time, there are few previous studies that emphasize observation of the effects resulting fromexposure to two or more substances. The aim of this study was to evaluate the combined effect of the mixture of herbicideGesapax 500® (ametrina 500 g/L) and Velpar K® (diuron 468 g/kg + hexazinone 132 g/kg) on zebrafish (Danio rerio). The testwas based on the Fish Embryo Toxicity (FET) from OECD, lasting 96 hours. The concentrations tested were: 0, 21.22, 29.52,41.08, 57.17 and 79.56 mg/L Gesapax 500 vs. 0, 15.21, 21.17, 29.46, 40.99 and 57.04 mg/L Velpar K. Tests were conductedin triplicate and evaluated daily. LC50-96h was determined to 41.705 ± 8.373 mg/L to Gesapax 500 and 55.460 ± 20.826 mg/Lto Velpar K. The mixture model that best describes the relationship between the two components is independent action andtoxicity of the mixture is dose-dependent, occurring antagonism at low doses and synergism at high doses. The endpointsedema, delay in general development and in yolk sac absorption and decrease in frequency of heart-beat rate were observedfrom the lower concentrations of the mixture. From the data obtained, it is concluded that the mixture of Velpar K and Gesapax500 is moderately toxic to zebrafish and that the endpoints evaluated were useful in determining its toxicity.