Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics

Detalhes bibliográficos
Autor(a) principal: Gaspar, Diana
Data de Publicação: 2015
Outros Autores: Freire, João M., Pacheco, Teresa R., Barata, João T., Castanho, Miguel A. R. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10451/23085
Resumo: © 2014 Elsevier B.V. All rights reserved.
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spelling Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanicsAnticancer peptideSolid tumorMembrane chargeApoptosisNanomechanical propertiesAtomic force microscopy© 2014 Elsevier B.V. All rights reserved.Supplementary data to this article can be found online at http://dx. doi.org/10.1016/j.bbamcr.2014.11.006Cancer remains a major cause of morbidity and mortality worldwide. Although progress has been made regarding chemotherapeutic agents, new therapies that combine increased selectivity and efficacy with low resistance are still needed. In the search for new anticancer agents, therapies based on biologically active peptides, in particular, antimicrobial peptides (AMPs), have attracted attention for their decreased resistance development and low cytotoxicity. Many AMPs have proved to be tumoricidal agents against human cancer cells, but their mode of action is still controversial. The existence of common properties shared by the membranes of bacteria and tumor cells points to similar lipid-targeting mechanisms in both cases. On the other hand, anticancer peptides (ACPs) also induce apoptosis and inhibit angiogenesis. Human neutrophil peptide-1 (HNP-1) is an endogenous AMP that has been implicated in different cellular phenomena such as tumor proliferation. The presence of HNP-1 in the serum/plasma of oncologic patients turns this peptide into a potential tumor biomarker. The present work reveals the different effects of HNP-1 on the biophysical and nanomechanical properties of solid and hematological tumor cells. Studies on cellular morphology, cellular stiffness, and membrane ultrastructure and charge using atomic force microscopy (AFM) and zeta potential measurements show a preferential binding of HNP-1 to solid tumor cells from human prostate adenocarcinoma when compared to human leukemia cells. AFM also reveals induction of apoptosis with cellular membrane defects at very low peptide concentrations. Understanding ACPsmode(s) of action will certainly open innovative pathways for drug development in cancer treatment.This work was supported by a grant from Laço (Portugal). Diana Gaspar and João Freire acknowledge FCT-MEC for fellowships SFRH/BPD/73500/2010 and SFRH/BD/70423/2010, respectivelyElsevierRepositório da Universidade de LisboaGaspar, DianaFreire, João M.Pacheco, Teresa R.Barata, João T.Castanho, Miguel A. R. B.2016-03-18T15:23:01Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23085engBiochimica et Biophysica Acta 1853 (2015) 308–3160006-300210.1016/j.bbamcr.2014.11.006metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:27:36Zoai:repositorio.ulisboa.pt:10451/23085Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:45:04.594016Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
title Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
spellingShingle Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
Gaspar, Diana
Anticancer peptide
Solid tumor
Membrane charge
Apoptosis
Nanomechanical properties
Atomic force microscopy
title_short Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
title_full Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
title_fullStr Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
title_full_unstemmed Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
title_sort Apoptotic human neutrophil peptide-1 anti-tumor activity revealed by cellular biomechanics
author Gaspar, Diana
author_facet Gaspar, Diana
Freire, João M.
Pacheco, Teresa R.
Barata, João T.
Castanho, Miguel A. R. B.
author_role author
author2 Freire, João M.
Pacheco, Teresa R.
Barata, João T.
Castanho, Miguel A. R. B.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gaspar, Diana
Freire, João M.
Pacheco, Teresa R.
Barata, João T.
Castanho, Miguel A. R. B.
dc.subject.por.fl_str_mv Anticancer peptide
Solid tumor
Membrane charge
Apoptosis
Nanomechanical properties
Atomic force microscopy
topic Anticancer peptide
Solid tumor
Membrane charge
Apoptosis
Nanomechanical properties
Atomic force microscopy
description © 2014 Elsevier B.V. All rights reserved.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-03-18T15:23:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23085
url http://hdl.handle.net/10451/23085
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta 1853 (2015) 308–316
0006-3002
10.1016/j.bbamcr.2014.11.006
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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