Development of a chromatographic method for simultaneous analysis of glutathione forms

Bibliographic Details
Main Author: Teixeira, Dulce
Publication Date: 2019
Other Authors: Coelho, Pedro, Prudêncio, Cristina, Azenha, Manuel, Vieira, Mónica
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.22/19011
Summary: Reduced glutathione (GSH)is the most abundant low molecular weight thiol-containing tripeptide (glycine, cysteine, and glutamate) which is synthesized in the cells. GSHplays critical roles in protecting cells from oxidative damage and the toxicity of xenobiotics. Besides, it is also involved in the regulation of intracellular redox homeostasis, which leads to its oxidation into oxidized glutathione (GSSG). Determining the ratio of GSH/GSSG in different biological samples is a major procedure for the evaluation of an individual’s oxidative status and can be a potential biomarker of oxidative stress.
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spelling Development of a chromatographic method for simultaneous analysis of glutathione formsReduced glutathione-GSHOxidized glutathione-GSSGHPLC-DADOxidative stressReduced glutathione (GSH)is the most abundant low molecular weight thiol-containing tripeptide (glycine, cysteine, and glutamate) which is synthesized in the cells. GSHplays critical roles in protecting cells from oxidative damage and the toxicity of xenobiotics. Besides, it is also involved in the regulation of intracellular redox homeostasis, which leads to its oxidation into oxidized glutathione (GSSG). Determining the ratio of GSH/GSSG in different biological samples is a major procedure for the evaluation of an individual’s oxidative status and can be a potential biomarker of oxidative stress.Escola Superior Saúde Instituto Politécnico PortoREPOSITÓRIO P.PORTOTeixeira, DulceCoelho, PedroPrudêncio, CristinaAzenha, ManuelVieira, Mónica2021-12-07T11:36:11Z2019-05-172019-05-17T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10400.22/19011enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-07T10:17:58Zoai:recipp.ipp.pt:10400.22/19011Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T00:47:17.775117Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of a chromatographic method for simultaneous analysis of glutathione forms
title Development of a chromatographic method for simultaneous analysis of glutathione forms
spellingShingle Development of a chromatographic method for simultaneous analysis of glutathione forms
Teixeira, Dulce
Reduced glutathione-GSH
Oxidized glutathione-GSSG
HPLC-DAD
Oxidative stress
title_short Development of a chromatographic method for simultaneous analysis of glutathione forms
title_full Development of a chromatographic method for simultaneous analysis of glutathione forms
title_fullStr Development of a chromatographic method for simultaneous analysis of glutathione forms
title_full_unstemmed Development of a chromatographic method for simultaneous analysis of glutathione forms
title_sort Development of a chromatographic method for simultaneous analysis of glutathione forms
author Teixeira, Dulce
author_facet Teixeira, Dulce
Coelho, Pedro
Prudêncio, Cristina
Azenha, Manuel
Vieira, Mónica
author_role author
author2 Coelho, Pedro
Prudêncio, Cristina
Azenha, Manuel
Vieira, Mónica
author2_role author
author
author
author
dc.contributor.none.fl_str_mv REPOSITÓRIO P.PORTO
dc.contributor.author.fl_str_mv Teixeira, Dulce
Coelho, Pedro
Prudêncio, Cristina
Azenha, Manuel
Vieira, Mónica
dc.subject.por.fl_str_mv Reduced glutathione-GSH
Oxidized glutathione-GSSG
HPLC-DAD
Oxidative stress
topic Reduced glutathione-GSH
Oxidized glutathione-GSSG
HPLC-DAD
Oxidative stress
description Reduced glutathione (GSH)is the most abundant low molecular weight thiol-containing tripeptide (glycine, cysteine, and glutamate) which is synthesized in the cells. GSHplays critical roles in protecting cells from oxidative damage and the toxicity of xenobiotics. Besides, it is also involved in the regulation of intracellular redox homeostasis, which leads to its oxidation into oxidized glutathione (GSSG). Determining the ratio of GSH/GSSG in different biological samples is a major procedure for the evaluation of an individual’s oxidative status and can be a potential biomarker of oxidative stress.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-17
2019-05-17T00:00:00Z
2021-12-07T11:36:11Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/19011
url http://hdl.handle.net/10400.22/19011
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola Superior Saúde Instituto Politécnico Porto
publisher.none.fl_str_mv Escola Superior Saúde Instituto Politécnico Porto
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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