Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids

Bibliographic Details
Main Author: Coelho, Sara Domingos
Publication Date: 2024
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/97015
Summary: Tese de mestrado, Biologia Molecular e Genética, 2024, Universidade de Lisboa, Faculdade de Ciências
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spelling Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipidsVitis viniferaPlasmopara viticolalípidos cloroplastidiaissinalização lipídicaloci de resistência (Rpvs)Teses de mestrado - 2024Departamento de Biologia VegetalTese de mestrado, Biologia Molecular e Genética, 2024, Universidade de Lisboa, Faculdade de CiênciasVitis vinifera is one of the most cultivated fruit crops in the world, being highly susceptible to Plasmopara viticola, the etiological agent of grapevine downy mildew, leading to heavy yield reductions and, consequently, to massive economic losses. To control this disease, preventive application of pesticides occurs throughout the season. Due to the environmental problems caused by pesticides and other chemicals, it is necessary to develop more sustainable control solutions. Over time, several studies have demonstrated the importance of lipids in the grapevine's response to pathogens. This group of molecules comprises components of cell membranes, surface lipids, which are the first barrier that pathogens encounter, and signaling molecules, involved in the cellular changes that occur following infection. The importance of chloroplast lipids and fatty acids in the outcome of the infection, being either associated to the jasmonic acid hormone signaling pathway or through the modification of membrane fluidity has also been highlighted. However, knowledge on the main modifications related to fatty acid and lipid composition at the chloroplastidial level is still scarce. In this work we characterized for the first time, the gene families that code enzymes involved in chloroplast lipid biosynthesis. We have also determined both fatty acid and lipid modulation events during the interaction with P. viticola. We also exploited gene expression modulation in an infection context, using grapevine genotypes that harbour different resistance to Plasmopara viticola loci (RPVs) and characterized lipid modulation in the Vitis vinifera cultivars Chardonnay (susceptible), Calardis blanc (Rpv3-1, Rpv3- 2), Regent (Rpv3-1), Sauvignac (Rpv3-3, Rpv10) and Solaris (Rpv3-1, Rpv12).Figueiredo, Andreia Cristina Silva Viegas Mata, 1980-Matos, Ana Rita Barreiro Alves de, 1975-Repositório da Universidade de LisboaCoelho, Sara Domingos202420242025-12-27T00:00:00Z2024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/97015enginfo:eu-repo/semantics/embargoedAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T16:31:30Zoai:repositorio.ulisboa.pt:10400.5/97015Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:18:08.000657Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
title Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
spellingShingle Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
Coelho, Sara Domingos
Vitis vinifera
Plasmopara viticola
lípidos cloroplastidiais
sinalização lipídica
loci de resistência (Rpvs)
Teses de mestrado - 2024
Departamento de Biologia Vegetal
title_short Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
title_full Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
title_fullStr Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
title_full_unstemmed Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
title_sort Developing molecular tools to improve grapevine resilience against pathogens: new insights on the role of chloroplasts lipids
author Coelho, Sara Domingos
author_facet Coelho, Sara Domingos
author_role author
dc.contributor.none.fl_str_mv Figueiredo, Andreia Cristina Silva Viegas Mata, 1980-
Matos, Ana Rita Barreiro Alves de, 1975-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Coelho, Sara Domingos
dc.subject.por.fl_str_mv Vitis vinifera
Plasmopara viticola
lípidos cloroplastidiais
sinalização lipídica
loci de resistência (Rpvs)
Teses de mestrado - 2024
Departamento de Biologia Vegetal
topic Vitis vinifera
Plasmopara viticola
lípidos cloroplastidiais
sinalização lipídica
loci de resistência (Rpvs)
Teses de mestrado - 2024
Departamento de Biologia Vegetal
description Tese de mestrado, Biologia Molecular e Genética, 2024, Universidade de Lisboa, Faculdade de Ciências
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024-01-01T00:00:00Z
2025-12-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/97015
url http://hdl.handle.net/10400.5/97015
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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