Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding

Bibliographic Details
Main Author: Pinho, C.J.
Publication Date: 2022
Other Authors: Lopes, E.P., Paupério, J., Gomes, I., Romeiras, Maria M., Vasconcelos, R.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/24094
Summary: Research Article
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spelling Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcodingCabo Verde Islandsdietgastrointestinal tracthouse mouseinvasive speciesinvertebratesnext-generation sequencingplantsResearch Article1. DNA metabarcoding is widely used to characterize the diet of species, and it becomes very relevant for biodiversity conservation, allowing the understanding of trophic chains and the impact of invasive species. The need for cost-effective biodiversity monitoring methods fostered advances in this technique. One question that arises is which sample type provides a better diet representation. 2. Therefore, with this study, we intended to evaluate if there were differences in diet estimates according to the section of the gastrointestinal tract analysed and which section(s) provided the best diet representation. Additionally, we intended to infer the ecological/economic impacts of an invader as a model of the potential effects in an originally mammal-free ecosystem. 3. We examined the gut contents of the house mouse Mus musculus introduced to Cabo Verde, considering three sections: stomach, small intestine, and large intestine. We applied a DNA-metabarcoding approach using two genetic markers, one specific for plants and another for invertebrates. 4. We showed that this invader consumed 131 taxa (73 plants and 58 invertebrates). We obtained significant differences in the composition of two of the three sections, with a higher incidence of invertebrates in the stomach and plants in the intestines. This may be due to stomach inhibitors acting on plants and/or to faster absorption of soft-body invertebrates compared to the plant fibers in the intestines. We verified that the impact of this invader in the ecosystem is predominantly negative, as at least 50% of the ingested items were native, endemic, or economically important taxa, and only 19% of the diet items were exotics 5. Overall, results showed the need to analyse only two gastrointestinal tract sections to obtain robust diet data, increasing the cost-effectiveness of the method. Furthermore, by uncovering the native taxa most frequently preyed on by mice, this DNA-metabarcoding approach allowed us to evaluate efficiently which are at the highest riskWileyRepositório da Universidade de LisboaPinho, C.J.Lopes, E.P.Paupério, J.Gomes, I.Romeiras, Maria M.Vasconcelos, R.2022-04-18T10:59:05Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24094engEcology and Evolution. 2022;12:e8638https://doi.org/10.1002/ece3.8638info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T16:12:53Zoai:repositorio.ulisboa.pt:10400.5/24094Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:06:07.842488Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
title Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
spellingShingle Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
Pinho, C.J.
Cabo Verde Islands
diet
gastrointestinal tract
house mouse
invasive species
invertebrates
next-generation sequencing
plants
title_short Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
title_full Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
title_fullStr Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
title_full_unstemmed Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
title_sort Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
author Pinho, C.J.
author_facet Pinho, C.J.
Lopes, E.P.
Paupério, J.
Gomes, I.
Romeiras, Maria M.
Vasconcelos, R.
author_role author
author2 Lopes, E.P.
Paupério, J.
Gomes, I.
Romeiras, Maria M.
Vasconcelos, R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pinho, C.J.
Lopes, E.P.
Paupério, J.
Gomes, I.
Romeiras, Maria M.
Vasconcelos, R.
dc.subject.por.fl_str_mv Cabo Verde Islands
diet
gastrointestinal tract
house mouse
invasive species
invertebrates
next-generation sequencing
plants
topic Cabo Verde Islands
diet
gastrointestinal tract
house mouse
invasive species
invertebrates
next-generation sequencing
plants
description Research Article
publishDate 2022
dc.date.none.fl_str_mv 2022-04-18T10:59:05Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24094
url http://hdl.handle.net/10400.5/24094
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ecology and Evolution. 2022;12:e8638
https://doi.org/10.1002/ece3.8638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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