Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?

Bibliographic Details
Main Author: Ramalho, Patrícia Alexandra Costa Ferreira
Publication Date: 2004
Other Authors: Paiva, Sandra, Paulo, Artur Cavaco, Casal, Margarida, Ramalho, Maria Teresa, Cardoso, M. Helena
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/2253
Summary: Poster apresentado na International Conference on Plasma Membrane Redox Systems and their Role in Biological Stress and Disease, 7, Asilomar, Califórnia, Estados Unidos da América, 14-18 Abril 2004.
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spelling Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?AzoreductaseFerric reductasePoster apresentado na International Conference on Plasma Membrane Redox Systems and their Role in Biological Stress and Disease, 7, Asilomar, Califórnia, Estados Unidos da América, 14-18 Abril 2004.Unspecific bacterial reduction of azo dyes is a widely studied process in correlation with the biological treatment of coloured waste waters but the enzyme system associated to this bacterial capability has never been positively identified. Several ascomycete yeast strains, isolated by our group from contaminated soils, display similar decolourising activities. A study of the yeast-mediated process in batch culture demonstrated that colour loss was due to a reductase activity, expressed constitutively, which could transform azo dyes into colourless amines. In order to get a better understanding of the azoreductase activity of yeast cells we selected a Saccharomyces cerevisiae strain (BLC0276) with a high decolourising capacity. A likely candidate might be the plasma membrane ferric reductase system. Both azoreductase and ferric reductase have peak activities in the late exponential growth phase, and their substrates -ferricyanide and soluble azo dyes - are impermeant to the cell plasma membrane. This hypothesis was confirmed through two different lines of evidence: the use of different inhibitors and the construction of mutants defective in the iron reductase system. Inhibitors like carbonylcyanide m-chlorophenylhydrazone (CCCP), diphenylene iodonium (DPI), p-chloromercuribenzoate (pCMB) and chloroquine (CQ), were tested. In most cases the percentage inhibition of both activities was similar. The genes Fre1 and Fre2 were deleted and the effect on the decolourising activity was analysed in the mutant strains. The effect of Fre2 deletion was negligible, but fre1 and fre1fre2 strains showed a much reduced decolourising capability. These results demonstrate the involvement of the ferric reductase system in the azo decolourising capacity of S. cerevisiae. However the capacity was not completely removed indicating that cells have an alternative reducing system.BIOEFTEX Project.Universidade do MinhoRamalho, Patrícia Alexandra Costa FerreiraPaiva, SandraPaulo, Artur CavacoCasal, MargaridaRamalho, Maria TeresaCardoso, M. Helena2004-042004-04-01T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/2253enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-15T01:17:04Zoai:repositorium.sdum.uminho.pt:1822/2253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:41:50.124764Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
title Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
spellingShingle Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
Ramalho, Patrícia Alexandra Costa Ferreira
Azoreductase
Ferric reductase
title_short Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
title_full Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
title_fullStr Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
title_full_unstemmed Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
title_sort Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?
author Ramalho, Patrícia Alexandra Costa Ferreira
author_facet Ramalho, Patrícia Alexandra Costa Ferreira
Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Ramalho, Maria Teresa
Cardoso, M. Helena
author_role author
author2 Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Ramalho, Maria Teresa
Cardoso, M. Helena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ramalho, Patrícia Alexandra Costa Ferreira
Paiva, Sandra
Paulo, Artur Cavaco
Casal, Margarida
Ramalho, Maria Teresa
Cardoso, M. Helena
dc.subject.por.fl_str_mv Azoreductase
Ferric reductase
topic Azoreductase
Ferric reductase
description Poster apresentado na International Conference on Plasma Membrane Redox Systems and their Role in Biological Stress and Disease, 7, Asilomar, Califórnia, Estados Unidos da América, 14-18 Abril 2004.
publishDate 2004
dc.date.none.fl_str_mv 2004-04
2004-04-01T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2253
url https://hdl.handle.net/1822/2253
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595479764500480

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