Towards a scalable bioprocess for rAAV production using a HeLa stable cell line

• Main Author: Escandell, José
• Publication Date: 2023
• Other Authors: Moura, Filipa, Carvalho, Sofia B., Silva, Ricardo J.S., Correia, Ricardo, Roldão, Antonio, Gomes-Alves, Patrícia, Alves, Paula M.
• Format: Article
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: http://hdl.handle.net/10362/165687
• topic: bioprocess development; cell line development; downstream processing; gene therapy; HeLaS3; recombinant adeno-associated viruses (rAAV); stable cell line; Biotechnology; Bioengineering; Applied Microbiology and Biotechnology

Funding Information: We thank Dr. Maria João Sebastião, Dr. Mafalda Dias, Maria Gonçalves and Mariana Antunes for their helpful discussion and experimental support. This work was funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), the Associate Laboratory LS4FUTURE (LA/P/0087/2020) and project PTDC/BTM-ORG/1383/2020, and by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 813453 and TRANSVAC2 No. 730964; J.M.E. is funded by Stimulus of Scientific Employment, Individual Support program (2020.01216.CEECIND) from FCT; F.M is funded by PhD fellowship (2022.11494.BD) from FCT. Images were generated with Biorender.com. Funding Information: We thank Dr. Maria João Sebastião, Dr. Mafalda Dias, Maria Gonçalves and Mariana Antunes for their helpful discussion and experimental support. This work was funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), the Associate Laboratory LS4FUTURE (LA/P/0087/2020) and project PTDC/BTM‐ORG/1383/2020, and by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska‐Curie grant agreement No. 813453 and TRANSVAC2 No. 813453; J.M.E. is funded by Stimulus of Scientific Employment, Individual Support program (2020.01216.CEECIND) from FCT; F.M is funded by PhD fellowship (2022.11494.BD) from FCT. Images were generated with Biorender.com . Publisher Copyright: © 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.