Structure of a dioxygen reduction enzyme from Desulfovibrio gigas
| Main Author: | |
|---|---|
| Publication Date: | 2000 |
| Other Authors: | , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10174/2099 |
Summary: | Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 Angstrom resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta -lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance. |
| id |
RCAP_c58d424f7666d529d3ae5d081c6bdb22 |
|---|---|
| oai_identifier_str |
oai:dspace.uevora.pt:10174/2099 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| repository_id_str |
https://opendoar.ac.uk/repository/7160 |
| spelling |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigasROODesulfovibrio gigasDesulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 Angstrom resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta -lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.2010-09-27T14:40:55Z2010-09-272000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29892 bytesapplication/pdfhttp://hdl.handle.net/10174/2099http://hdl.handle.net/10174/2099eng1041-10457NATURE STRUCTURAL BIOLOGYlivrendndndndndndndndndndndndndnd552Frazão, CarlosSilva, GabrielaGomes, ClaudioMatias, PedroCoelho, RSieker, LMacedo, SLiu, MYOliveira, SolangeTeixeira, MiguelXavier, António V.Rodrigues-Pousada, ClaudinaCarrondo, M. ArméniaLeGall, Jeaninfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-01-03T18:38:17Zoai:dspace.uevora.pt:10174/2099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T11:50:58.989684Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| title |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| spellingShingle |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas Frazão, Carlos ROO Desulfovibrio gigas |
| title_short |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| title_full |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| title_fullStr |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| title_full_unstemmed |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| title_sort |
Structure of a dioxygen reduction enzyme from Desulfovibrio gigas |
| author |
Frazão, Carlos |
| author_facet |
Frazão, Carlos Silva, Gabriela Gomes, Claudio Matias, Pedro Coelho, R Sieker, L Macedo, S Liu, MY Oliveira, Solange Teixeira, Miguel Xavier, António V. Rodrigues-Pousada, Claudina Carrondo, M. Arménia LeGall, Jean |
| author_role |
author |
| author2 |
Silva, Gabriela Gomes, Claudio Matias, Pedro Coelho, R Sieker, L Macedo, S Liu, MY Oliveira, Solange Teixeira, Miguel Xavier, António V. Rodrigues-Pousada, Claudina Carrondo, M. Arménia LeGall, Jean |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Frazão, Carlos Silva, Gabriela Gomes, Claudio Matias, Pedro Coelho, R Sieker, L Macedo, S Liu, MY Oliveira, Solange Teixeira, Miguel Xavier, António V. Rodrigues-Pousada, Claudina Carrondo, M. Arménia LeGall, Jean |
| dc.subject.por.fl_str_mv |
ROO Desulfovibrio gigas |
| topic |
ROO Desulfovibrio gigas |
| description |
Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 Angstrom resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta -lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance. |
| publishDate |
2000 |
| dc.date.none.fl_str_mv |
2000-01-01T00:00:00Z 2010-09-27T14:40:55Z 2010-09-27 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/2099 http://hdl.handle.net/10174/2099 |
| url |
http://hdl.handle.net/10174/2099 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1041-1045 7 NATURE STRUCTURAL BIOLOGY livre nd nd nd nd nd nd nd nd nd nd nd nd nd nd 552 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
29892 bytes application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia instacron:RCAAP |
| instname_str |
FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| collection |
Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| repository.name.fl_str_mv |
Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
| repository.mail.fl_str_mv |
info@rcaap.pt |
| _version_ |
1833592288454901760 |