Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine

Bibliographic Details
Main Author: Saldanha, C.
Publication Date: 2012
Other Authors: Freitas, T., Almeida, J. P.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/10682
Summary: © 2012 Elsevier Inc. All rights reserved.
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spelling Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholineNitric oxideS-nitrosogluthationeErythrocyte deformability© 2012 Elsevier Inc. All rights reserved.Aims: The objectives of this study were to evaluate the effects of high fibrinogen concentration on erythrocyte deformability on mobilization of nitric oxide (NO) and of its metabolites in the presence of acetylcholine (ACh) in healthy human blood samples. Main methods: Levels of NO were evaluated by amperometric method. Nitrite, nitrate and S-nitrosoglutathione (GSNO) were measured using the spectrophotometric Griess reaction. Erythrocyte deformability was determined using the Rheodyn SSD laser diffractometer. Key findings: In the presence of high concentrations of fibrinogen and ACh (10 μM) in the blood samples from healthy humans the erythrocyte nitrites, nitrates and GSNO concentrations increased without significant changes in NO efflux.Mobilization of NO in erythrocytes' presence was enhanced in the presence of ACh and high fibrinogen levels. Significance: These results suggest that during inflammation when both ACh and high levels of fibrinogen are present, NO delivery by erythrocytes might be compromised by their NO scavenging ability that acts as a compensatory mechanism against the overproduced NO by endothelial inducible nitric oxide synthase.This study was supported by grants from the FCT — Fundação para a Ciência e a Tecnologia (project reference PTDC/SAU-OSM/73449/2006)ElsevierRepositório da Universidade de LisboaSaldanha, C.Freitas, T.Almeida, J. P.2014-03-05T15:08:40Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10682engLife Sciences 91 (2012) 1017–10220024-3205http://dx.doi.org/10.1016/j.lfs.2012.06.035metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:06:36Zoai:repositorio.ulisboa.pt:10451/10682Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:35:07.698048Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
title Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
spellingShingle Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
Saldanha, C.
Nitric oxide
S-nitrosogluthatione
Erythrocyte deformability
title_short Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
title_full Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
title_fullStr Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
title_full_unstemmed Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
title_sort Fibrinogen effects on erythrocyte nitric oxide mobilization in presence of acetylcholine
author Saldanha, C.
author_facet Saldanha, C.
Freitas, T.
Almeida, J. P.
author_role author
author2 Freitas, T.
Almeida, J. P.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Saldanha, C.
Freitas, T.
Almeida, J. P.
dc.subject.por.fl_str_mv Nitric oxide
S-nitrosogluthatione
Erythrocyte deformability
topic Nitric oxide
S-nitrosogluthatione
Erythrocyte deformability
description © 2012 Elsevier Inc. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-05T15:08:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10682
url http://hdl.handle.net/10451/10682
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences 91 (2012) 1017–1022
0024-3205
http://dx.doi.org/10.1016/j.lfs.2012.06.035
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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