Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles

Bibliographic Details
Main Author: Maia, J
Publication Date: 2020
Other Authors: Otake, AH, Poças, J, Carvalho, AS, Beck, HC, Magalhães, A, Matthiesen, R, Strano Moraes, MC, Costa-Silva, B
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/10216/145270
Summary: Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM cells and induce the expansion of this cell population. Transcriptomic characterization of these cells shows that PC-EVs upregulate IgG and IgA genes, which have been linked to the presence of monocytes/macrophages in tumor microenvironments. We also report here the transcriptional downregulation of genes linked to monocyte/macrophage activation, trafficking, and expression of inflammatory molecules. Together, these results show for the first time the existence of a PC–BM communication axis mediated by EVs with a potential role in PC tumor microenvironments.
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spelling Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular VesiclesCancerExosomesExtracellular vesiclesMacrophagesMetastasisMonocytesPancreatic cancerTumor microenvironmentPancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM cells and induce the expansion of this cell population. Transcriptomic characterization of these cells shows that PC-EVs upregulate IgG and IgA genes, which have been linked to the presence of monocytes/macrophages in tumor microenvironments. We also report here the transcriptional downregulation of genes linked to monocyte/macrophage activation, trafficking, and expression of inflammatory molecules. Together, these results show for the first time the existence of a PC–BM communication axis mediated by EVs with a potential role in PC tumor microenvironments.Frontiers Media20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/145270eng2296-634X10.3389/fcell.2020.592518Maia, JOtake, AHPoças, JCarvalho, ASBeck, HCMagalhães, AMatthiesen, RStrano Moraes, MCCosta-Silva, Binfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-27T16:59:05Zoai:repositorio-aberto.up.pt:10216/145270Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T21:59:24.118362Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
title Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
spellingShingle Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
Maia, J
Cancer
Exosomes
Extracellular vesicles
Macrophages
Metastasis
Monocytes
Pancreatic cancer
Tumor microenvironment
title_short Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
title_full Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
title_fullStr Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
title_full_unstemmed Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
title_sort Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
author Maia, J
author_facet Maia, J
Otake, AH
Poças, J
Carvalho, AS
Beck, HC
Magalhães, A
Matthiesen, R
Strano Moraes, MC
Costa-Silva, B
author_role author
author2 Otake, AH
Poças, J
Carvalho, AS
Beck, HC
Magalhães, A
Matthiesen, R
Strano Moraes, MC
Costa-Silva, B
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maia, J
Otake, AH
Poças, J
Carvalho, AS
Beck, HC
Magalhães, A
Matthiesen, R
Strano Moraes, MC
Costa-Silva, B
dc.subject.por.fl_str_mv Cancer
Exosomes
Extracellular vesicles
Macrophages
Metastasis
Monocytes
Pancreatic cancer
Tumor microenvironment
topic Cancer
Exosomes
Extracellular vesicles
Macrophages
Metastasis
Monocytes
Pancreatic cancer
Tumor microenvironment
description Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM cells and induce the expansion of this cell population. Transcriptomic characterization of these cells shows that PC-EVs upregulate IgG and IgA genes, which have been linked to the presence of monocytes/macrophages in tumor microenvironments. We also report here the transcriptional downregulation of genes linked to monocyte/macrophage activation, trafficking, and expression of inflammatory molecules. Together, these results show for the first time the existence of a PC–BM communication axis mediated by EVs with a potential role in PC tumor microenvironments.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/145270
url https://hdl.handle.net/10216/145270
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-634X
10.3389/fcell.2020.592518
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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