A new method for extraction of exopolymers from activated sludges

Bibliographic Details
Main Author: Azeredo, Joana
Publication Date: 1998
Other Authors: Oliveira, Rosário, Lazarova, V.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/1401
Summary: The exopolymeric matrix that surrounds the biomass greatly contributes to the efficiency of activated sludge systems. To study the composition of this matrix a preliminary extraction method is required. In this work three extraction methods (vapor, sonication and combined treatment by sonication and Dowex resin) were used and compared with a new method which uses glutaraldehyde. The extraction effectiveness was estimated by measuring the total protein, the total organic carbon in the extracted solutions and by determining the monosaccharide constituents of the extracted polysaccharides. The proposed new method proved to be the most suitable because it extracted a great quantity of organic matter without disrupting the cells.
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spelling A new method for extraction of exopolymers from activated sludgesActivated sludgeBiopolymer extractionExopolymersGlutaraldehydeIon exchangePolysaccharidesProteinsSonicationVaporScience & TechnologyThe exopolymeric matrix that surrounds the biomass greatly contributes to the efficiency of activated sludge systems. To study the composition of this matrix a preliminary extraction method is required. In this work three extraction methods (vapor, sonication and combined treatment by sonication and Dowex resin) were used and compared with a new method which uses glutaraldehyde. The extraction effectiveness was estimated by measuring the total protein, the total organic carbon in the extracted solutions and by determining the monosaccharide constituents of the extracted polysaccharides. The proposed new method proved to be the most suitable because it extracted a great quantity of organic matter without disrupting the cells.ElsevierUniversidade do MinhoAzeredo, JoanaOliveira, RosárioLazarova, V.19981998-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/1401eng"Water science and technology". ISSN 0273-1223. 37:4/5 (1998) 367-370.0273-122310.1016/S0273-1223(98)00132-2info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:37:04Zoai:repositorium.sdum.uminho.pt:1822/1401Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:29:58.149263Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv A new method for extraction of exopolymers from activated sludges
title A new method for extraction of exopolymers from activated sludges
spellingShingle A new method for extraction of exopolymers from activated sludges
Azeredo, Joana
Activated sludge
Biopolymer extraction
Exopolymers
Glutaraldehyde
Ion exchange
Polysaccharides
Proteins
Sonication
Vapor
Science & Technology
title_short A new method for extraction of exopolymers from activated sludges
title_full A new method for extraction of exopolymers from activated sludges
title_fullStr A new method for extraction of exopolymers from activated sludges
title_full_unstemmed A new method for extraction of exopolymers from activated sludges
title_sort A new method for extraction of exopolymers from activated sludges
author Azeredo, Joana
author_facet Azeredo, Joana
Oliveira, Rosário
Lazarova, V.
author_role author
author2 Oliveira, Rosário
Lazarova, V.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Azeredo, Joana
Oliveira, Rosário
Lazarova, V.
dc.subject.por.fl_str_mv Activated sludge
Biopolymer extraction
Exopolymers
Glutaraldehyde
Ion exchange
Polysaccharides
Proteins
Sonication
Vapor
Science & Technology
topic Activated sludge
Biopolymer extraction
Exopolymers
Glutaraldehyde
Ion exchange
Polysaccharides
Proteins
Sonication
Vapor
Science & Technology
description The exopolymeric matrix that surrounds the biomass greatly contributes to the efficiency of activated sludge systems. To study the composition of this matrix a preliminary extraction method is required. In this work three extraction methods (vapor, sonication and combined treatment by sonication and Dowex resin) were used and compared with a new method which uses glutaraldehyde. The extraction effectiveness was estimated by measuring the total protein, the total organic carbon in the extracted solutions and by determining the monosaccharide constituents of the extracted polysaccharides. The proposed new method proved to be the most suitable because it extracted a great quantity of organic matter without disrupting the cells.
publishDate 1998
dc.date.none.fl_str_mv 1998
1998-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/1401
url https://hdl.handle.net/1822/1401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Water science and technology". ISSN 0273-1223. 37:4/5 (1998) 367-370.
0273-1223
10.1016/S0273-1223(98)00132-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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