Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots

Detalhes bibliográficos
Autor(a) principal: Almeida, Ema
Data de Publicação: 2022
Outros Autores: Soares, Sofia, Gonçalves, Joana, Rosado, Tiago, Fernández, Nicolás, Rodilla, Jesus M., Passarinha, Luís A., Barroso, Mário, Gallardo, Eugénia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/143800
Resumo: Funding: LA/P/0140/2020 (i4HB) being carried out by National Funds by Foundation for Science and Technology (FCT) and co-financed by community funds. T. Rosado thanks the Centre for Competences in Cloud Computing for the scholarship with reference C4_WP2.6_M1—Bioinformatics; UBIMEDICAL— CENTER-01-0145-FEDER-000019-C4—Centre for Competences in Cloud Computing, European Regional Development Fund (FEDER) and Centro Regional Operational Program (Centro 2020). S. Soares and J. Gonçalves acknowledge the FCT for the PhD fellowships with references SFRH BD/148753/2019. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Stability of Cocaine, Opiates, and Metabolites in Dried Saliva SpotsCocaineDried saliva spotsGC–MS/MSOpiatesOral fluidStabilityAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistrySDG 3 - Good Health and Well-beingFunding: LA/P/0140/2020 (i4HB) being carried out by National Funds by Foundation for Science and Technology (FCT) and co-financed by community funds. T. Rosado thanks the Centre for Competences in Cloud Computing for the scholarship with reference C4_WP2.6_M1—Bioinformatics; UBIMEDICAL— CENTER-01-0145-FEDER-000019-C4—Centre for Competences in Cloud Computing, European Regional Development Fund (FEDER) and Centro Regional Operational Program (Centro 2020). S. Soares and J. Gonçalves acknowledge the FCT for the PhD fellowships with references SFRH BD/148753/2019. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Drug abuse still represents a global problem, and it is associated with an increased risk of diseases, injuries, and deaths. Cocaine (COC) and opiates are the most abused drugs and account for a significant number of fatalities. Therefore, it is important to develop methods capable of effectively identifying and quantifying these substances. The present study aims to evaluate the longterm stability of COC, ecgonine methylester (EME), benzoylecgonine (BEG), cocaethylene (COET), norcocaine (NCOC), morphine (MOR), codeine (COD) and 6-monoacetylmorphine (6-MAM) in oral fluid samples. The analytes of interest were isolated from the matrix (50 µL) using the dried saliva spots (DSS) sampling approach and were subsequently analyzed by gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The parameters that could influence the stability of the target compounds were studied, and these were storage temperature, light, use of preservatives (and respective concentrations), and time. The effects of each parameter were evaluated using the design of experiments (DOE) approach. The stability of the target analytes was improved when the DSS were stored at room temperature, in the presence of light and using 1% sodium fluoride. The best conditions were then adopted for the DSS storage and long-term stability was assessed. COD was only stable for 1 day, EME was stable for 3 days, COC, COET, NCOC and 6-MAM were stable for 7 days, MOR for 14 days and BEG remained stable throughout the study (136 days). This is the first study that evaluates the stability of these compounds in oral fluid samples after application in DSS cards, and optimizes the conditions in order to improve their stability.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNAlmeida, EmaSoares, SofiaGonçalves, JoanaRosado, TiagoFernández, NicolásRodilla, Jesus M.Passarinha, Luís A.Barroso, MárioGallardo, Eugénia2022-09-16T22:28:18Z2022-01-192022-01-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/143800eng1420-3049PURE: 46582123https://doi.org/10.3390/molecules27030641info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:05:08Zoai:run.unl.pt:10362/143800Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:35:45.368863Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
title Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
spellingShingle Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
Almeida, Ema
Cocaine
Dried saliva spots
GC–MS/MS
Opiates
Oral fluid
Stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
title_short Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
title_full Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
title_fullStr Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
title_full_unstemmed Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
title_sort Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots
author Almeida, Ema
author_facet Almeida, Ema
Soares, Sofia
Gonçalves, Joana
Rosado, Tiago
Fernández, Nicolás
Rodilla, Jesus M.
Passarinha, Luís A.
Barroso, Mário
Gallardo, Eugénia
author_role author
author2 Soares, Sofia
Gonçalves, Joana
Rosado, Tiago
Fernández, Nicolás
Rodilla, Jesus M.
Passarinha, Luís A.
Barroso, Mário
Gallardo, Eugénia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Almeida, Ema
Soares, Sofia
Gonçalves, Joana
Rosado, Tiago
Fernández, Nicolás
Rodilla, Jesus M.
Passarinha, Luís A.
Barroso, Mário
Gallardo, Eugénia
dc.subject.por.fl_str_mv Cocaine
Dried saliva spots
GC–MS/MS
Opiates
Oral fluid
Stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
topic Cocaine
Dried saliva spots
GC–MS/MS
Opiates
Oral fluid
Stability
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
description Funding: LA/P/0140/2020 (i4HB) being carried out by National Funds by Foundation for Science and Technology (FCT) and co-financed by community funds. T. Rosado thanks the Centre for Competences in Cloud Computing for the scholarship with reference C4_WP2.6_M1—Bioinformatics; UBIMEDICAL— CENTER-01-0145-FEDER-000019-C4—Centre for Competences in Cloud Computing, European Regional Development Fund (FEDER) and Centro Regional Operational Program (Centro 2020). S. Soares and J. Gonçalves acknowledge the FCT for the PhD fellowships with references SFRH BD/148753/2019. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-16T22:28:18Z
2022-01-19
2022-01-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143800
url http://hdl.handle.net/10362/143800
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
PURE: 46582123
https://doi.org/10.3390/molecules27030641
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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