An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation

Detalhes bibliográficos
Autor(a) principal: Ermida, Catarina
Data de Publicação: 2023
Outros Autores: Rosa, Joana, Cunha, Eugénia, Ferreira, Maria Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10400.26/49138
Resumo: Postmortem interval (PMI) estimation constitutes a challenge for forensic anthropologists.The application of the luminol technique as a preliminary test for PMI estimation is considered easy to use and inexpensive. The objective of our study is to validate luminol chemiluminescence testing through the assessment of intra- and interobserver error. Our sample included 266 human clavicles, with known PMIs ranging between 2655 days and 450 years. After sample preparation, luminol was applied, and the results were observed by two different observers. The intensity of the reaction was measured using a binary scale and a 5-level scale, according to the increasing degree of chemiluminescence. The Kappa statistic was used for the assessment of the intra- and interobserver agreement. The obtained results showed total interobserver error agreement regarding the binary scale and a K = 0.98 (95% CI: 0.97–0.99) regarding the 5-level scale. Additionally, Observer 1 and Observer 2 obtained a 100% agreement concerning the binary scale and a K = 1 (95%CI 0.99–1) and K = 0.99 (95% CI 0.99–1), respectively, concerning the 5-level scale. According to our research, it is possible to conclude that luminol chemiluminescence testing is suitable as a rapid diagnostic test, revealing this method as practically independent of observation error
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spelling An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval EstimationPostmortem intervalLuminolChemiluminescenceInterobserver errorPostmortem interval (PMI) estimation constitutes a challenge for forensic anthropologists.The application of the luminol technique as a preliminary test for PMI estimation is considered easy to use and inexpensive. The objective of our study is to validate luminol chemiluminescence testing through the assessment of intra- and interobserver error. Our sample included 266 human clavicles, with known PMIs ranging between 2655 days and 450 years. After sample preparation, luminol was applied, and the results were observed by two different observers. The intensity of the reaction was measured using a binary scale and a 5-level scale, according to the increasing degree of chemiluminescence. The Kappa statistic was used for the assessment of the intra- and interobserver agreement. The obtained results showed total interobserver error agreement regarding the binary scale and a K = 0.98 (95% CI: 0.97–0.99) regarding the 5-level scale. Additionally, Observer 1 and Observer 2 obtained a 100% agreement concerning the binary scale and a K = 1 (95%CI 0.99–1) and K = 0.99 (95% CI 0.99–1), respectively, concerning the 5-level scale. According to our research, it is possible to conclude that luminol chemiluminescence testing is suitable as a rapid diagnostic test, revealing this method as practically independent of observation errorMDPIRepositório ComumErmida, CatarinaRosa, JoanaCunha, EugéniaFerreira, Maria Teresa2024-01-19T14:00:31Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/49138eng2673-675610.3390/forensicsci3040044info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-05-10T04:23:46Zoai:comum.rcaap.pt:10400.26/49138Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T07:06:12.022330Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
title An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
spellingShingle An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
Ermida, Catarina
Postmortem interval
Luminol
Chemiluminescence
Interobserver error
title_short An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
title_full An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
title_fullStr An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
title_full_unstemmed An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
title_sort An Assessment of Intra- and Interobserver Error in Luminol Chemiluminescence as a Presumptive Test for Postmortem Interval Estimation
author Ermida, Catarina
author_facet Ermida, Catarina
Rosa, Joana
Cunha, Eugénia
Ferreira, Maria Teresa
author_role author
author2 Rosa, Joana
Cunha, Eugénia
Ferreira, Maria Teresa
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Ermida, Catarina
Rosa, Joana
Cunha, Eugénia
Ferreira, Maria Teresa
dc.subject.por.fl_str_mv Postmortem interval
Luminol
Chemiluminescence
Interobserver error
topic Postmortem interval
Luminol
Chemiluminescence
Interobserver error
description Postmortem interval (PMI) estimation constitutes a challenge for forensic anthropologists.The application of the luminol technique as a preliminary test for PMI estimation is considered easy to use and inexpensive. The objective of our study is to validate luminol chemiluminescence testing through the assessment of intra- and interobserver error. Our sample included 266 human clavicles, with known PMIs ranging between 2655 days and 450 years. After sample preparation, luminol was applied, and the results were observed by two different observers. The intensity of the reaction was measured using a binary scale and a 5-level scale, according to the increasing degree of chemiluminescence. The Kappa statistic was used for the assessment of the intra- and interobserver agreement. The obtained results showed total interobserver error agreement regarding the binary scale and a K = 0.98 (95% CI: 0.97–0.99) regarding the 5-level scale. Additionally, Observer 1 and Observer 2 obtained a 100% agreement concerning the binary scale and a K = 1 (95%CI 0.99–1) and K = 0.99 (95% CI 0.99–1), respectively, concerning the 5-level scale. According to our research, it is possible to conclude that luminol chemiluminescence testing is suitable as a rapid diagnostic test, revealing this method as practically independent of observation error
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
2024-01-19T14:00:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/49138
url http://hdl.handle.net/10400.26/49138
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2673-6756
10.3390/forensicsci3040044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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