Development of osteogenic cell sheets for bone tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Pirraco, Rogério
Data de Publicação: 2011
Outros Autores: Obokata, Haruko, Iwata, Takanori, Marques, A. P., Tsuneda, Satoshi, Yamato, Masayuki, Reis, R. L., Okano, T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/1822/12862
Resumo: The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of cell sheet (CS) engineering as a possible way to overcome some of these obstacles. Osteogenic CSs were fabricated by culturing rat bone marrow stromal cells in thermoresponsive culture dishes. The CSs were recovered from the dishes using a low-temperature treatment and then were implanted subcutaneously in nude mice. New bone formation was verified from day 7 post-transplantation using X-ray, microcomputed tomography, and histological analysis. The presence of a vascularized marrow was also verified in the newly formed bone after 6 weeks of transplantation. Further, osteocytes were found in this newly formed tissue, supporting the conclusion that mature bone was formed after ectopically transplanting osteogenic CSs. These results therefore confirm the great potentiality of CS engineering to be used in bone tissue engineering applications
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spelling Development of osteogenic cell sheets for bone tissue engineering applicationsCell sheetsBone tissue engineeringScience & TechnologyThe use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of cell sheet (CS) engineering as a possible way to overcome some of these obstacles. Osteogenic CSs were fabricated by culturing rat bone marrow stromal cells in thermoresponsive culture dishes. The CSs were recovered from the dishes using a low-temperature treatment and then were implanted subcutaneously in nude mice. New bone formation was verified from day 7 post-transplantation using X-ray, microcomputed tomography, and histological analysis. The presence of a vascularized marrow was also verified in the newly formed bone after 6 weeks of transplantation. Further, osteocytes were found in this newly formed tissue, supporting the conclusion that mature bone was formed after ectopically transplanting osteogenic CSs. These results therefore confirm the great potentiality of CS engineering to be used in bone tissue engineering applicationsThis study was partially supported by Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology "Cell Sheet Tissue Engineering Center (CSTEC)" and the Global COE program, Multidisciplinary Education and Research Center for Regenerative Medicine (MER-CREM), from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.Financial support to R. P. Pirraco by the Portuguese Foundation for Science and Technology (FCT) through the PhD grant SFRH/BD/44893/2008 is also acknowledged.Mary Ann LiebertUniversidade do MinhoPirraco, RogérioObokata, HarukoIwata, TakanoriMarques, A. P.Tsuneda, SatoshiYamato, MasayukiReis, R. L.Okano, T.2011-042011-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12862eng2152-494710.1089/ten.tea.2010.047021275832info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:38:57Zoai:repositorium.sdum.uminho.pt:1822/12862Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:25:14.357481Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of osteogenic cell sheets for bone tissue engineering applications
title Development of osteogenic cell sheets for bone tissue engineering applications
spellingShingle Development of osteogenic cell sheets for bone tissue engineering applications
Pirraco, Rogério
Cell sheets
Bone tissue engineering
Science & Technology
title_short Development of osteogenic cell sheets for bone tissue engineering applications
title_full Development of osteogenic cell sheets for bone tissue engineering applications
title_fullStr Development of osteogenic cell sheets for bone tissue engineering applications
title_full_unstemmed Development of osteogenic cell sheets for bone tissue engineering applications
title_sort Development of osteogenic cell sheets for bone tissue engineering applications
author Pirraco, Rogério
author_facet Pirraco, Rogério
Obokata, Haruko
Iwata, Takanori
Marques, A. P.
Tsuneda, Satoshi
Yamato, Masayuki
Reis, R. L.
Okano, T.
author_role author
author2 Obokata, Haruko
Iwata, Takanori
Marques, A. P.
Tsuneda, Satoshi
Yamato, Masayuki
Reis, R. L.
Okano, T.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pirraco, Rogério
Obokata, Haruko
Iwata, Takanori
Marques, A. P.
Tsuneda, Satoshi
Yamato, Masayuki
Reis, R. L.
Okano, T.
dc.subject.por.fl_str_mv Cell sheets
Bone tissue engineering
Science & Technology
topic Cell sheets
Bone tissue engineering
Science & Technology
description The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of cell sheet (CS) engineering as a possible way to overcome some of these obstacles. Osteogenic CSs were fabricated by culturing rat bone marrow stromal cells in thermoresponsive culture dishes. The CSs were recovered from the dishes using a low-temperature treatment and then were implanted subcutaneously in nude mice. New bone formation was verified from day 7 post-transplantation using X-ray, microcomputed tomography, and histological analysis. The presence of a vascularized marrow was also verified in the newly formed bone after 6 weeks of transplantation. Further, osteocytes were found in this newly formed tissue, supporting the conclusion that mature bone was formed after ectopically transplanting osteogenic CSs. These results therefore confirm the great potentiality of CS engineering to be used in bone tissue engineering applications
publishDate 2011
dc.date.none.fl_str_mv 2011-04
2011-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/12862
url http://hdl.handle.net/1822/12862
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2152-4947
10.1089/ten.tea.2010.0470
21275832
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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