Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages

Bibliographic Details
Main Author: Ventura, Célia
Publication Date: 2023
Other Authors: Pinto, Fátima, Lourenço, Ana Filipa, Pedrosa, Jorge F.S., Fernandes, Susete N., da Rosa, Rafaela R., Godinho, Maria Helena, Ferreira, Paulo J.T., Louro, Henriqueta, Silva, Maria João
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/157764
Summary: Funding Information: The work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The authors also acknowledge the support of FCT/MCTES to the following projects: LA/P/0037/2020, UIDP/50025/2020 and UIDB/50025/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N, CIEPQPF (UIDB/00102/2020) and ToxOmics, NMS-UNL (UIDP/00009/2020; UIDB/00009/2020). Thanks also to European Cooperation in Science & Technology (COST) Action: CA21159—Understanding interaction light—biological surfaces: possibility for new electronic materials and devices (PhoBioS); Inpactus Project—innovative products and technologies from eucalyptus (Project No. 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 No. 246/AXIS II/2017). Publisher Copyright: © 2023 by the authors.
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spelling Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophagesbiocompatibilitynanocrystalline cellulosenanofibrillated celluloserespiratory effectssafety assessmentBioengineeringSDG 3 - Good Health and Well-beingFunding Information: The work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The authors also acknowledge the support of FCT/MCTES to the following projects: LA/P/0037/2020, UIDP/50025/2020 and UIDB/50025/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N, CIEPQPF (UIDB/00102/2020) and ToxOmics, NMS-UNL (UIDP/00009/2020; UIDB/00009/2020). Thanks also to European Cooperation in Science & Technology (COST) Action: CA21159—Understanding interaction light—biological surfaces: possibility for new electronic materials and devices (PhoBioS); Inpactus Project—innovative products and technologies from eucalyptus (Project No. 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 No. 246/AXIS II/2017). Publisher Copyright: © 2023 by the authors.Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential.Centre for Toxicogenomics and Human Health (ToxOmics)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)Faculdade de Ciências e Tecnologia (FCT)RUNVentura, CéliaPinto, FátimaLourenço, Ana FilipaPedrosa, Jorge F.S.Fernandes, Susete N.da Rosa, Rafaela R.Godinho, Maria HelenaFerreira, Paulo J.T.Louro, HenriquetaSilva, Maria João2023-09-13T22:18:27Z2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/157764eng2306-5354PURE: 71088564https://doi.org/10.3390/bioengineering10080986info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-07-22T01:36:43Zoai:run.unl.pt:10362/157764Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:44:58.610088Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
spellingShingle Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
Ventura, Célia
biocompatibility
nanocrystalline cellulose
nanofibrillated cellulose
respiratory effects
safety assessment
Bioengineering
SDG 3 - Good Health and Well-being
title_short Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_full Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_fullStr Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_full_unstemmed Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_sort Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
author Ventura, Célia
author_facet Ventura, Célia
Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F.S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J.T.
Louro, Henriqueta
Silva, Maria João
author_role author
author2 Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F.S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J.T.
Louro, Henriqueta
Silva, Maria João
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centre for Toxicogenomics and Human Health (ToxOmics)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
DCM - Departamento de Ciência dos Materiais
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Ventura, Célia
Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F.S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J.T.
Louro, Henriqueta
Silva, Maria João
dc.subject.por.fl_str_mv biocompatibility
nanocrystalline cellulose
nanofibrillated cellulose
respiratory effects
safety assessment
Bioengineering
SDG 3 - Good Health and Well-being
topic biocompatibility
nanocrystalline cellulose
nanofibrillated cellulose
respiratory effects
safety assessment
Bioengineering
SDG 3 - Good Health and Well-being
description Funding Information: The work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The authors also acknowledge the support of FCT/MCTES to the following projects: LA/P/0037/2020, UIDP/50025/2020 and UIDB/50025/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N, CIEPQPF (UIDB/00102/2020) and ToxOmics, NMS-UNL (UIDP/00009/2020; UIDB/00009/2020). Thanks also to European Cooperation in Science & Technology (COST) Action: CA21159—Understanding interaction light—biological surfaces: possibility for new electronic materials and devices (PhoBioS); Inpactus Project—innovative products and technologies from eucalyptus (Project No. 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 No. 246/AXIS II/2017). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-13T22:18:27Z
2023-08
2023-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/157764
url http://hdl.handle.net/10362/157764
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2306-5354
PURE: 71088564
https://doi.org/10.3390/bioengineering10080986
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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