Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Costa, Sofia Judite
Data de Publicação: 2010
Outros Autores: Castro, António, Domingues, Lucília
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/1822/35494
Resumo: Recombinant protein production is a useful technology for therapeutic and diagnostic applications. The bacterium Escherichia coli is widely used for the bioproduction of proteins, but still presents some drawbacks. Recombinant proteins with biomedical interest have proved difficult to express properly in this host system, resulting in insoluble protein aggregates. Fusion protein technology has been applied to overcome these drawbacks and to optimize protein expression in E. coli. This research aims at the evaluation of a novel fusion system (Fh8 and H tags) effects on protein production. Target proteins used in this work present biomedical interest and results obtained here showed that these novel fusion tags increased protein production yields. This novel fusion system is a promising tool for the production of recombinant proteins with biomedical interest.
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spelling Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coliRecombinant proteinsEscherichia coliFusion systemOverexpressionRecombinant protein production is a useful technology for therapeutic and diagnostic applications. The bacterium Escherichia coli is widely used for the bioproduction of proteins, but still presents some drawbacks. Recombinant proteins with biomedical interest have proved difficult to express properly in this host system, resulting in insoluble protein aggregates. Fusion protein technology has been applied to overcome these drawbacks and to optimize protein expression in E. coli. This research aims at the evaluation of a novel fusion system (Fh8 and H tags) effects on protein production. Target proteins used in this work present biomedical interest and results obtained here showed that these novel fusion tags increased protein production yields. This novel fusion system is a promising tool for the production of recombinant proteins with biomedical interest.Universidade do MinhoCosta, Sofia JuditeCastro, AntónioDomingues, Lucília2010-102010-10-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/35494engCosta, S. M.; Castro, A.; Domingues, Lucília, Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli. Semana da Escola de Engenharia 2010. Guimarães, Portugal, Outubro, 2010. ISBN: 978-972-8692-59-9978-972-8692-59-9info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:28:33Zoai:repositorium.sdum.uminho.pt:1822/35494Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T14:49:30.157467Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
title Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
spellingShingle Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
Costa, Sofia Judite
Recombinant proteins
Escherichia coli
Fusion system
Overexpression
title_short Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
title_full Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
title_fullStr Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
title_full_unstemmed Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
title_sort Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli
author Costa, Sofia Judite
author_facet Costa, Sofia Judite
Castro, António
Domingues, Lucília
author_role author
author2 Castro, António
Domingues, Lucília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, Sofia Judite
Castro, António
Domingues, Lucília
dc.subject.por.fl_str_mv Recombinant proteins
Escherichia coli
Fusion system
Overexpression
topic Recombinant proteins
Escherichia coli
Fusion system
Overexpression
description Recombinant protein production is a useful technology for therapeutic and diagnostic applications. The bacterium Escherichia coli is widely used for the bioproduction of proteins, but still presents some drawbacks. Recombinant proteins with biomedical interest have proved difficult to express properly in this host system, resulting in insoluble protein aggregates. Fusion protein technology has been applied to overcome these drawbacks and to optimize protein expression in E. coli. This research aims at the evaluation of a novel fusion system (Fh8 and H tags) effects on protein production. Target proteins used in this work present biomedical interest and results obtained here showed that these novel fusion tags increased protein production yields. This novel fusion system is a promising tool for the production of recombinant proteins with biomedical interest.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
2010-10-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/35494
url http://hdl.handle.net/1822/35494
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Costa, S. M.; Castro, A.; Domingues, Lucília, Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli. Semana da Escola de Engenharia 2010. Guimarães, Portugal, Outubro, 2010. ISBN: 978-972-8692-59-9
978-972-8692-59-9
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dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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