Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments

Bibliographic Details
Main Author: Duarte, Ricardo Franco
Publication Date: 2011
Other Authors: Carreto, Laura, Cambon, Brigitte, Dequin, Sylvie, Santos, Manuel A. S., Casal, Margarida, Schuller, Dorit Elisabeth
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/13512
Summary: Apresentação efetuada nas 36.ª Jornadas Portuguesas de Genética, Coimbra, 2011
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spelling Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environmentsComparative genomicsSaccharomyces cerevisiaeCommercial yeastApresentação efetuada nas 36.ª Jornadas Portuguesas de Genética, Coimbra, 2011The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every year at the harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery and can be recovered from locations in close proximity (10-200m) (Valero et al., 2005). Their permanence in natural environments induced genetic changes such as loss of heterozygosity, microsatellite expansion and size variations of chromosomes III and/or VI. These changes were not found among a group of isolates that were used as a control and that derived from the commercial “mother” strain (Schuller et al., 2007). The objective of the present study was to evaluate genome variations among four isolates of the commercial strain S. cerevisiae Zymaflore VL1 that were re-isolated from vineyards surrounding the wineries where this strain was applied, in comparison to the commercial “mother” strain by the use of comparative genome hybridization on array (aCGH). These approaches were carried out as previously described (Carreto, et al., 2008). Hierarchical cluster analysis showed some genome variability between isolates recovered from the environment and the commercial VL1 reference strain. Using one-way ANOVA we identified more than 50 genes with significant differences between the environmental isolates and the original VL1 strain. Multi-class significance analysis (SAM) for two-class unpaired comparisons revealed a group of 130 genes with significantly reduced fold changes in the recovered strains. The most relevant genes (PAU2, PAU3 and PAU5) belonged to the seripauperin family. They are located in subtelomeric chromosomal regions, where gene families are maintained that serve for adaptive purposes. These genes are involved in the adaptation of yeast cells to stress during alcoholic fermentations and are highly upregulated during early stages of wine fermentation. Our studies showed that genetic changes occur among isogenic strains of the commercial yeast S. cerevisiae Zymaflore VL1 that were re-isolated from environments close to vineyards where this strain is used as starter yeast. The depletion of several genes may reflect adaptive mechanisms that strains undergo as response to the ecological conditions of vineyards that are very distinct from must fermentation environments.Fundação para a Ciência e a Tecnologia (FCT)Universidade do MinhoDuarte, Ricardo FrancoCarreto, LauraCambon, BrigitteDequin, SylvieSantos, Manuel A. S.Casal, MargaridaSchuller, Dorit Elisabeth20112011-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/13512enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T07:34:32Zoai:repositorium.sdum.uminho.pt:1822/13512Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:31:51.873470Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
title Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
spellingShingle Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
Duarte, Ricardo Franco
Comparative genomics
Saccharomyces cerevisiae
Commercial yeast
title_short Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
title_full Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
title_fullStr Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
title_full_unstemmed Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
title_sort Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments
author Duarte, Ricardo Franco
author_facet Duarte, Ricardo Franco
Carreto, Laura
Cambon, Brigitte
Dequin, Sylvie
Santos, Manuel A. S.
Casal, Margarida
Schuller, Dorit Elisabeth
author_role author
author2 Carreto, Laura
Cambon, Brigitte
Dequin, Sylvie
Santos, Manuel A. S.
Casal, Margarida
Schuller, Dorit Elisabeth
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Duarte, Ricardo Franco
Carreto, Laura
Cambon, Brigitte
Dequin, Sylvie
Santos, Manuel A. S.
Casal, Margarida
Schuller, Dorit Elisabeth
dc.subject.por.fl_str_mv Comparative genomics
Saccharomyces cerevisiae
Commercial yeast
topic Comparative genomics
Saccharomyces cerevisiae
Commercial yeast
description Apresentação efetuada nas 36.ª Jornadas Portuguesas de Genética, Coimbra, 2011
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/13512
url http://hdl.handle.net/1822/13512
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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repository.mail.fl_str_mv info@rcaap.pt
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