Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling

Bibliographic Details
Main Author: Casalou, Cristina
Publication Date: 2019
Other Authors: Faustino, Alexandra, Silva, Fernanda, Ferreira, Inês C., Vaqueirinho, Daniela, Ferreira, Andreia, Castanheira, Pedro, Barona, Teresa, Ramalho, José S., Serpa, Jacinta, Félix, Ana, Barral, Duarte C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.3390/cancers11101461
Summary: This work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.
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spelling Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signalingActin cytoskeletonArl proteinsCancer progressionCell-extracellular matrix adhesionIntegrinsOncologyCancer ResearchSDG 3 - Good Health and Well-beingThis work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.Breast cancer is the first cause of cancer-related mortality among women worldwide, according to the most recent estimates. This mortality is mainly caused by the tumors’ ability to form metastases. Cancer cell migration and invasion are essential for metastasis and rely on the interplay between actin cytoskeleton remodeling and cell adhesion. Therefore, understanding the mechanisms by which cancer cell invasion is controlled may provide new strategies to impair cancer progression. We investigated the role of the ADP-ribosylation factor (Arf)-like (Arl) protein Arl13b in breast cancer cell migration and invasion in vitro, using breast cancer cell lines and in vivo, using mouse orthotopic models. We show that Arl13b silencing inhibits breast cancer cell migration and invasion in vitro, as well as cancer progression in vivo. We also observed that Arl13b is upregulated in breast cancer cell lines and patient tissue samples. Moreover, we found that Arl13b localizes to focal adhesions (FAs) and interacts with β3-integrin. Upon Arl13b silencing, β3-integrin cell surface levels and FA size are increased and integrin-mediated signaling is inhibited. Therefore, we uncover a role for Arl13b in breast cancer cell migration and invasion and provide a new mechanism for how ARL13B can function as an oncogene, through the modulation of integrin-mediated signaling.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCasalou, CristinaFaustino, AlexandraSilva, FernandaFerreira, Inês C.Vaqueirinho, DanielaFerreira, AndreiaCastanheira, PedroBarona, TeresaRamalho, José S.Serpa, JacintaFélix, AnaBarral, Duarte C.2019-11-15T05:27:27Z2019-10-012019-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/cancers11101461eng2072-6694PURE: 15224183http://www.scopus.com/inward/record.url?scp=85073476079&partnerID=8YFLogxKhttps://doi.org/10.3390/cancers11101461info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-10-21T01:35:59Zoai:run.unl.pt:10362/87326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:13:28.916932Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
title Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
spellingShingle Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
Casalou, Cristina
Actin cytoskeleton
Arl proteins
Cancer progression
Cell-extracellular matrix adhesion
Integrins
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
title_short Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
title_full Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
title_fullStr Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
title_full_unstemmed Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
title_sort Arl13b regulates breast cancer cell migration and invasion by controlling integrin-mediated signaling
author Casalou, Cristina
author_facet Casalou, Cristina
Faustino, Alexandra
Silva, Fernanda
Ferreira, Inês C.
Vaqueirinho, Daniela
Ferreira, Andreia
Castanheira, Pedro
Barona, Teresa
Ramalho, José S.
Serpa, Jacinta
Félix, Ana
Barral, Duarte C.
author_role author
author2 Faustino, Alexandra
Silva, Fernanda
Ferreira, Inês C.
Vaqueirinho, Daniela
Ferreira, Andreia
Castanheira, Pedro
Barona, Teresa
Ramalho, José S.
Serpa, Jacinta
Félix, Ana
Barral, Duarte C.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Casalou, Cristina
Faustino, Alexandra
Silva, Fernanda
Ferreira, Inês C.
Vaqueirinho, Daniela
Ferreira, Andreia
Castanheira, Pedro
Barona, Teresa
Ramalho, José S.
Serpa, Jacinta
Félix, Ana
Barral, Duarte C.
dc.subject.por.fl_str_mv Actin cytoskeleton
Arl proteins
Cancer progression
Cell-extracellular matrix adhesion
Integrins
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
topic Actin cytoskeleton
Arl proteins
Cancer progression
Cell-extracellular matrix adhesion
Integrins
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
description This work was supported by PhD fellowships from Fundação para a Ciência e a Tecnologia(FCT) to A. Faustino, A. Ferreira and P.C. (PD/BD/105898/2014, PD/BD/135506/2018 and PD/BD/128339/2017, respectively), a post-doctoral fellowship from FCT to C.C. (SFRH/BPD/78561/2011), the FCT Investigator Program to D.C.B. (IF/00501/2014/CP1252/CT0001), and grants from FCT (PTDC/BIM-MEC/4905/2014) and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-15T05:27:27Z
2019-10-01
2019-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.3390/cancers11101461
url https://doi.org/10.3390/cancers11101461
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-6694
PURE: 15224183
http://www.scopus.com/inward/record.url?scp=85073476079&partnerID=8YFLogxK
https://doi.org/10.3390/cancers11101461
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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