New insights into functional regulation in MS-based drug profiling

Bibliographic Details
Main Author: Carvalho, A.S.
Publication Date: 2016
Other Authors: Molina, H., Matthiesen, Rune
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.18/3818
Summary: We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.
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spelling New insights into functional regulation in MS-based drug profilingLC-MSProteomicsBiochemistryCell BiologyCancerComputational biology and bioinformaticsChromatography–mass SpectrometryGenomica FuncionalGenómica Funcional e EstruturalWe present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.Nature Publishing GroupRepositório Científico do Instituto Nacional de SaúdeCarvalho, A.S.Molina, H.Matthiesen, Rune2016-05-24T16:33:03Z2016-01-082016-01-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3818eng2045-2322doi: 10.1038/srep18826.info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-26T14:18:47Zoai:repositorio.insa.pt:10400.18/3818Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T21:33:05.878617Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv New insights into functional regulation in MS-based drug profiling
title New insights into functional regulation in MS-based drug profiling
spellingShingle New insights into functional regulation in MS-based drug profiling
Carvalho, A.S.
LC-MS
Proteomics
Biochemistry
Cell Biology
Cancer
Computational biology and bioinformatics
Chromatography–mass Spectrometry
Genomica Funcional
Genómica Funcional e Estrutural
title_short New insights into functional regulation in MS-based drug profiling
title_full New insights into functional regulation in MS-based drug profiling
title_fullStr New insights into functional regulation in MS-based drug profiling
title_full_unstemmed New insights into functional regulation in MS-based drug profiling
title_sort New insights into functional regulation in MS-based drug profiling
author Carvalho, A.S.
author_facet Carvalho, A.S.
Molina, H.
Matthiesen, Rune
author_role author
author2 Molina, H.
Matthiesen, Rune
author2_role author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Carvalho, A.S.
Molina, H.
Matthiesen, Rune
dc.subject.por.fl_str_mv LC-MS
Proteomics
Biochemistry
Cell Biology
Cancer
Computational biology and bioinformatics
Chromatography–mass Spectrometry
Genomica Funcional
Genómica Funcional e Estrutural
topic LC-MS
Proteomics
Biochemistry
Cell Biology
Cancer
Computational biology and bioinformatics
Chromatography–mass Spectrometry
Genomica Funcional
Genómica Funcional e Estrutural
description We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-24T16:33:03Z
2016-01-08
2016-01-08T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3818
url http://hdl.handle.net/10400.18/3818
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
doi: 10.1038/srep18826.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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