Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis
| Main Author: | |
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| Publication Date: | 2021 |
| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/133558 |
Summary: | 50204076]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 Stringari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosisPublic Health, Environmental and Occupational HealthInfectious DiseasesSDG 3 - Good Health and Well-beingMicrobiologia Médica50204076]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 Stringari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background Regulatory T cells (Tregs) play a critical role during Mycobacterium tuberculosis (Mtb) infection, modulating host responses while neutralizing excessive inflammation. However, their impact on regulating host protective immunity is not completely understood. Here, we demonstrate that Treg cells abrogate the in vitro microbicidal activity against Mtb. Methods We evaluated the in vitro microbicidal activity of peripheral blood mononuclear cells (PBMCs) from patients with active tuberculosis (TB), individuals with latent tuberculosis infection (LTBI, TST+/IGRA+) and healthy control (HC, TST-/IGRA-) volunteers. PBMCs, depleted or not of CD4+CD25+ T-cells, were analyzed to determine frequency and influence on microbicidal activity during in vitro Mtb infection with four clinical isolates (S1, S5, R3, and R6) and one reference strain (H37Rv). Results The frequency of CD4+CD25highFoxP3+ cells were significantly higher in Mtb infected whole blood cultures from both TB patients and LTBI individuals when compared to HC. Data from CD4+CD25+ T-cells depletion demonstrate that increase of CD4+CD25highFoxP3+ is associated with an impairment of Th-1 responses and a diminished in vitro microbicidal activity of LTBI and TB groups. Conclusions Tregs restrict host anti-mycobacterial immunity during active disease and latent infection and thereby may contribute to both disease progression and pathogen persistence.Instituto de Higiene e Medicina Tropical (IHMT)Individual Health Care (IHC)Global Health and Tropical Medicine (GHTM)RUNStringari, Lorenzzo LyrioCovre, Luciana Polacoda Silva, Flávia Dias Coelhode Oliveira, Vivian LeiteCampana, Maria CarolinaHadad, David JamilPalaci, MoisésSalgame, PadminiDietze, ReynaldoGomes, Daniel Cláudio de OliveiraRibeiro-Rodrigues, Rodrigo2022-02-24T23:21:16Z2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/133558eng1935-2727PURE: 33794672https://doi.org/10.1371/journal.pntd.0009605info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:59:48Zoai:run.unl.pt:10362/133558Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:30:45.097576Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| title |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| spellingShingle |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis Stringari, Lorenzzo Lyrio Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being Microbiologia Médica |
| title_short |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| title_full |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| title_fullStr |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| title_full_unstemmed |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| title_sort |
Increase of cd4+cd25highfoxp3+ cells impairs in vitro human microbicidal activity against mycobacterium tuberculosis during latent and acute pulmonary tuberculosis |
| author |
Stringari, Lorenzzo Lyrio |
| author_facet |
Stringari, Lorenzzo Lyrio Covre, Luciana Polaco da Silva, Flávia Dias Coelho de Oliveira, Vivian Leite Campana, Maria Carolina Hadad, David Jamil Palaci, Moisés Salgame, Padmini Dietze, Reynaldo Gomes, Daniel Cláudio de Oliveira Ribeiro-Rodrigues, Rodrigo |
| author_role |
author |
| author2 |
Covre, Luciana Polaco da Silva, Flávia Dias Coelho de Oliveira, Vivian Leite Campana, Maria Carolina Hadad, David Jamil Palaci, Moisés Salgame, Padmini Dietze, Reynaldo Gomes, Daniel Cláudio de Oliveira Ribeiro-Rodrigues, Rodrigo |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Individual Health Care (IHC) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
Stringari, Lorenzzo Lyrio Covre, Luciana Polaco da Silva, Flávia Dias Coelho de Oliveira, Vivian Leite Campana, Maria Carolina Hadad, David Jamil Palaci, Moisés Salgame, Padmini Dietze, Reynaldo Gomes, Daniel Cláudio de Oliveira Ribeiro-Rodrigues, Rodrigo |
| dc.subject.por.fl_str_mv |
Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being Microbiologia Médica |
| topic |
Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being Microbiologia Médica |
| description |
50204076]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 Stringari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07 2021-07-01T00:00:00Z 2022-02-24T23:21:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/133558 |
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http://hdl.handle.net/10362/133558 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1935-2727 PURE: 33794672 https://doi.org/10.1371/journal.pntd.0009605 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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16 application/pdf |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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