Production of chitin-glucan complex by Pichia pastoris

Bibliographic Details
Main Author: Araújo, Diana Filipa Vieira
Publication Date: 2013
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/10897
Summary: Dissertation for the Degree of Master in Biotechnology
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spelling Production of chitin-glucan complex by Pichia pastorisChitinChitin-glucan complexPichia pastorisCarbon sourceResiduesXyloseDissertation for the Degree of Master in BiotechnologyThe yeast Pichia pastoris produces chitin-glucan complex (CGC), as a cell wall component. CGC is composed of two types of biopolymers, chitin and β-glucans, which confer it great potential for use in the food, cosmetic and pharmaceutical industries. CGC hydrolysis, allows obtaining chitin/chitosan and glucans individually. The chitin and chitosan obtained from CGC have the considerable advantage of being of non-animal origin, which further extends their applications. In last year’s, the production of CGC by Pichia pastoris was realized with glycerol as sole carbon source, achieving high cell density. In this study, different substrates were tested for cultivation of P. pastoris and CGC production. In the first part, mixtures of glucose and xylose, in varying proportions, were tested. Since glucose and xylose are two of the main sugar components of lignocellulosic wastes, the ability of P. pastoris to use them as carbon sources would allow their valorization into value-added products. In the second part, several wastes and byproducts generated by different industries were tested for their suitability as substrates for P. pastoris cultivation. In study of the glucose/xylose mixtures, the best performance was achieved in batch bioreactor experiments with 25% xylose in the medium (20 gL-1 of xylose and 60 gL-1 of glucose), where 35.25 gL-1 biomass was obtained in 64 hours of cultivation. CGC content in the cell wall reached 15% with a volumetric productivity of 0.085 gL-1.h-1. The molar ratio of chitin:β-glucan in the extracted biopolymer was 47:53, higher than obtained with crude glycerol (16:84). In the second part of study, several wastes and byproducts (used cooking oil, sugarcane molasses, cheese whey, waste paper and spent coffee grounds) were tested. The results show that P. pastoris presented low biomass concentration using any of these substrates. Nevertheless, in batch bioreactor experiments the best results were achieved with sugarcane molasses, where 17.78 gL-1 biomass were obtained with a CGC content of 17%. Among the tested substrates, the mixtures of glucose/xylose appear to be the most promising due the good CGC production obtained and the high glucosamine molar fraction in produced polymer. This study opens the hypothesis of utilization of lignocellulosic materials with xylose percentages up to 50%.Faculdade de Ciencias e TecnologiaFreitas, Maria FilomenaReis, Maria d'AscensãoRUNAraújo, Diana Filipa Vieira2013-12-19T10:29:24Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10897TID:201755475enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:14:43Zoai:run.unl.pt:10362/10897Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:45:46.383048Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Production of chitin-glucan complex by Pichia pastoris
title Production of chitin-glucan complex by Pichia pastoris
spellingShingle Production of chitin-glucan complex by Pichia pastoris
Araújo, Diana Filipa Vieira
Chitin
Chitin-glucan complex
Pichia pastoris
Carbon source
Residues
Xylose
title_short Production of chitin-glucan complex by Pichia pastoris
title_full Production of chitin-glucan complex by Pichia pastoris
title_fullStr Production of chitin-glucan complex by Pichia pastoris
title_full_unstemmed Production of chitin-glucan complex by Pichia pastoris
title_sort Production of chitin-glucan complex by Pichia pastoris
author Araújo, Diana Filipa Vieira
author_facet Araújo, Diana Filipa Vieira
author_role author
dc.contributor.none.fl_str_mv Freitas, Maria Filomena
Reis, Maria d'Ascensão
RUN
dc.contributor.author.fl_str_mv Araújo, Diana Filipa Vieira
dc.subject.por.fl_str_mv Chitin
Chitin-glucan complex
Pichia pastoris
Carbon source
Residues
Xylose
topic Chitin
Chitin-glucan complex
Pichia pastoris
Carbon source
Residues
Xylose
description Dissertation for the Degree of Master in Biotechnology
publishDate 2013
dc.date.none.fl_str_mv 2013-12-19T10:29:24Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10897
TID:201755475
url http://hdl.handle.net/10362/10897
identifier_str_mv TID:201755475
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciencias e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciencias e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596172363628544

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