Translocating the blood-brain barrier using electrostatics

Bibliographic Details
Main Author: Ribeiro, Marta M. B.
Publication Date: 2012
Other Authors: Domingues, Marco M., Freire, João M., Santos, Nuno C., Castanho, Miguel A. R. B.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/7309
Summary: Copyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
id RCAP_4e0ba4d4d6b01b0156a7666c3bb5c114
oai_identifier_str oai:repositorio.ulisboa.pt:10451/7309
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Translocating the blood-brain barrier using electrostaticsBlood-brain barrierDrug targetingBlood cellsCell surface chargeZeta-potentialCopyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.Mammalian cell membranes regulate homeostasis, protein activity, and cell signaling. The charge at the membrane surface has been correlated with these key events. Although mammalian cells are known to be slightly anionic, quantitative information on the membrane charge and the importance of electrostatic interactions in pharmacokinetics and pharmacodynamics remain elusive. Recently, we reported for the first time that brain endothelial cells (EC) are more negatively charged than human umbilical cord cells, using zeta-potential measurements by dynamic light scattering. Here, we hypothesize that anionicity is a key feature of the blood-brain barrier (BBB) and contributes to select which compounds cross into the brain. For the sake of comparison, we also studied the membrane surface charge of blood components—red blood cells (RBC), platelets, and peripheral blood mononuclear cells (PBMC).To further quantitatively correlate the negative zeta-potential values with membrane charge density, model membranes with different percentages of anionic lipids were also evaluated. From all the cells tested, brain cell membranes are the most anionic and those having their lipids mostly exposed, which explains why lipophilic cationic compounds are more prone to cross the blood-brain barrier.Fundação para a Ciência e Tecnologia — Ministério da Educação e Ciência (FCT-MEC, Portugal) is acknowledged for funding (including fellowships SFRH/BD/42158/2007 to Marta M.B. Ribeiro, SFRH/BD/41750/2007 to Marco M. Domingues and SFRH/BD/70423/2010 to João M. Freire) and project PTDC/QUI-BIQ/119509/2010. Marie Curie Industry-Academia Partnerships and Pathways (European Commission) is also acknowledged for funding (FP7-PEOPLE-2007-3-1-IAPP, Project 230654).Frontiers Research FoundationRepositório da Universidade de LisboaRibeiro, Marta M. B.Domingues, Marco M.Freire, João M.Santos, Nuno C.Castanho, Miguel A. R. B.2012-11-28T16:11:20Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/7309engFrontiers in Cellular Neuroscience October 2012 | Volume6 | Article44 | 1-71662-5102http://dx.doi.org/10.3389/fncel.2012.00044info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:55:42Zoai:repositorio.ulisboa.pt:10451/7309Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:31:14.603201Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Translocating the blood-brain barrier using electrostatics
title Translocating the blood-brain barrier using electrostatics
spellingShingle Translocating the blood-brain barrier using electrostatics
Ribeiro, Marta M. B.
Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
title_short Translocating the blood-brain barrier using electrostatics
title_full Translocating the blood-brain barrier using electrostatics
title_fullStr Translocating the blood-brain barrier using electrostatics
title_full_unstemmed Translocating the blood-brain barrier using electrostatics
title_sort Translocating the blood-brain barrier using electrostatics
author Ribeiro, Marta M. B.
author_facet Ribeiro, Marta M. B.
Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
author_role author
author2 Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ribeiro, Marta M. B.
Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
dc.subject.por.fl_str_mv Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
topic Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
description Copyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-28T16:11:20Z
2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/7309
url http://hdl.handle.net/10451/7309
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Cellular Neuroscience October 2012 | Volume6 | Article44 | 1-7
1662-5102
http://dx.doi.org/10.3389/fncel.2012.00044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601391954755584

Similar Items